Identification, Validation and Classification of the Genus Phyllanthus in Nigeria Using ITS Genetic Marker and the Taxonomic Implication
International Journal of Genetics and Genomics
Volume 3, Issue 1, February 2015, Pages: 1-7
Received: Jan. 30, 2015; Accepted: Feb. 11, 2015; Published: Feb. 16, 2015
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Authors
Daniel Azubuike Awomukwu, Department of Biological Sciences, Federal University, Otuoke, Bayelsa State, Nigeria
Bio Louis Nyananyo, Department of Plant Science and Biotechnology, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria
Paula Spies, Department of Genetics, University of the Free State, Bloemfontein, Free State, South Africa
Bulelani Londoloza Sizani, Department of Genetics, University of the Free State, Bloemfontein, Free State, South Africa
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Abstract
An extraction, purification, PCR amplification and sequencing of DNA from five species of Phyllanthus in Nigeria namely P. amarus Schum and Thonn, P. urinaria Linn., P. odontadenius Mull-Arg., P. niruroides Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were carried out using nuclear ribosomal Internal Transcribed Spacer (ITS 4-5) genetic marker to identify unknown Phyllanthus species. The nuclear region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on GenBank and BoldSystems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and BoldSystems while P. amarus Schum and Thonn, P. muellerianus (O. Ktze) Excel and P. urinaria Linn. were clearly compatible with other works. The sequence data were analyzed and classified with tree-based analyses of Mr.Bayes 3.2.1. in order to reveal their phylogenetic relationship. Results of the nucleotide sequences and fragment analysis were published on BoldSystems for barcoding as non-coding marker translation matrix.
Keywords
Phyllanthus species, DNA Extraction, PCR Amplification, Nucleotide Sequences, Gen Bank, Bold Systems, ITS, Molecular Phylogenetics
To cite this article
Daniel Azubuike Awomukwu, Bio Louis Nyananyo, Paula Spies, Bulelani Londoloza Sizani, Identification, Validation and Classification of the Genus Phyllanthus in Nigeria Using ITS Genetic Marker and the Taxonomic Implication, International Journal of Genetics and Genomics. Vol. 3, No. 1, 2015, pp. 1-7. doi: 10.11648/j.ijgg.20150301.11
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