Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes
International Journal of Genetics and Genomics
Volume 4, Issue 2, April 2016, Pages: 5-10
Received: Mar. 20, 2016; Accepted: Apr. 1, 2016; Published: Apr. 16, 2016
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Abdulrahman Lado, Department of Agronomy, Bayero University, Kano, Nigeria
Muhammad Auwal Hussaini, Department of Agronomy, Bayero University, Kano, Nigeria
Alpha Yaya Kamara, International Institute of Tropical Agriculture, Kano Station, Nigeria
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In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.
AHAS Enzyme, Imazaquin, Herbicide Tolerance and Cowpea Genotypes
To cite this article
Abdulrahman Lado, Muhammad Auwal Hussaini, Alpha Yaya Kamara, Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes, International Journal of Genetics and Genomics. Vol. 4, No. 2, 2016, pp. 5-10. doi: 10.11648/j.ijgg.20160402.11
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