Characterization of Phenotypic Variability of Sudanese Gum Arabic Tree Acacia Senegal var. senegal Using Multiprimer Random Amplificatıon of Polymorphic DNA (RAPD)
International Journal of Genetics and Genomics
Volume 4, Issue 3, June 2016, Pages: 20-23
Received: May 21, 2016;
Accepted: Jun. 8, 2016;
Published: Jun. 20, 2016
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Saad Samah Mahgoub Hassan Mohammed, Graduate School of Natural and Applied Sciences, University of Ankara, Ankara, Turkey
Mukhtar Moawia Mohammed, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan
Warag Essam Eelldin, Faculty of Forestry, University of Khartoum, Khartoum, Sudan
Elamin Hassan Basher, Ministry of Higher Education and Scientific Research, Khartoum, Sudan
Aycan Murat, Graduate School of Natural and Applied Sciences, University of Ankara, Ankara, Turkey
Yıldız Mustafa, Department of Field Crops, Faculty of Agriculture, University of Ankara, Ankara, Turkey
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Acacia Senegal var. senegal (Gum Arabic) as it is known world-wide, is one of the most important trees in Sudan with valuable contribution to the national income export commodity. Gum Arabic is an important ingridunt in several medicinal and food products. Little is known on the genetic markers and diversity of Acacia senegal. To our knowledge, this is the first study represents attempt to characterize genotypic variability of Acacia senegal var. senegal. The aim of this study was the molecular characterization of Acacia senegal var senegal using RAPD technique as molecular marker and to correlate the results obtained from genetic marker with the production and the geographical distribution of the trees. Acacia seeds were collected from different geographical areas of Sudan. Seeds of trees Acacia senegal var. senegal were cultivated in small pots. The seeds were germinated for 4 to 6 weeks then the new leaves were collected for DNA extraction using CTAB protocol. DNA was also extracted from the roots. Two octamer RAPD primers were evaluated for amplification of Acacia DNA. Phylogenetic tree was constructed based on RAPD-PCR which clustered the trees from each geographic area as separate group that were genetically related. The data showed a common ancestor of the three clusters suggesting.
Acacia Senegal var. senegal, Molecular Characterization, RAPD
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Saad Samah Mahgoub Hassan Mohammed,
Mukhtar Moawia Mohammed,
Warag Essam Eelldin,
Elamin Hassan Basher,
Characterization of Phenotypic Variability of Sudanese Gum Arabic Tree Acacia Senegal var. senegal Using Multiprimer Random Amplificatıon of Polymorphic DNA (RAPD), International Journal of Genetics and Genomics.
Vol. 4, No. 3,
2016, pp. 20-23.
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Odee D. W., Wilson J. and Cavers S. (2012) Prospects for genetic improvement of Acacia senegal. Can molecular markers approaches deliver better gum yield and quality? Gum arabic. Royal Society of Chemistry, Cambridge, pp 99–109.
Diallo A. M., Nielsen L. R., Hansen J. K., Ræbild A. and Kjær E. D. (2015). Study of quantitative genetics of gum arabic production complicated by variability in ploidy level of Acacia senegal (L.) Willd. Tree Genetics & Genomes, 11(4), 1-13.
Ræbild A., Larsen A. S., Jensen J. S, Ouedraogo M., De Groote S., Van Damme P., Bayala J., Diallo B. O., Sanou H., Kalinganire A. and Kjær E. D. (2011) Advances in domestication of indigenous fruit trees in the West African Sahel. New For 41: 297–315
2003 ICARDA Laboratory manual.
Sambrook I., Frilsch E. F. and Maniatis T. (1989). Molecular Cloning: A Laboratory Manual, 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
D'amato M. E. and Corach O. (1997). Population genetic structure in the fresh water anomuran aesla jujuyana by RAPO Analysis.
Armstrong J. S., Gibbs A. J., Peackail R. and Weiller G. (1994). The RAPOistance Package, Australian National University, Camberra, Australia, http://life.anu.au/molcular/software/rapd.html.
Rohlf F. J. (1994). NTSYS-pc Numerical taxonomy and multivariate analysis system. Exeter Software, New York.
Semagn K., Bjornstad A. and Ndjiondjop M. N. (2006). An overview of molecular marker methods for plants. African Journal of Biotechnology, ISSN 1684-5315 © 2006 Academic Journals, 5 (25): 2540-2568.
Williams I. G. K., Kubelik A. R., Livak K. L., Rafaiski I. A. and Tingey S. V. (1990). DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research, 18: 6531-6535.
Guyeri H. P. (2005). DNA Isolation and RAPD Analysis of Cladophora, Spirogyra, Bryopsis, with focus on Derbesia. [online]. LBS 145, Spring 2005, Michigan State University. US. https://www.msu. edu/-nguyen81Ilbs145.htm
Caetano-Arıolles G. (2004). DNA amplifieation fingerprinting. A forum for DNA marker methodologies: University ci Illinois at Urbana-Champaign. http://www.cropsei.uiue.edu/faeulty/gea/kaffe/tools1dafhtml.
Williams L. (1999). Biogeographic Fine-tuning Helps Weed Biocontrol. Bioeontrol News and Information: Volume 20 (2).
Gray A., Odee D., Cavers S., Wilson J., Telford A., Grant F., Diouf M., Ochieng J., Grant F. and Stott A. (2013). Does geographic origin dictate ecological strategies in Acacia senegal (L.) Willd.? Evidence from carbon and nitrogen stable isotopes. Plant Soil 369: 479–496.