CTLA-4 Gene Polymorphism in Women with Idiopathic Recurrent Pregnancy Loss
International Journal of Genetics and Genomics
Volume 4, Issue 4, August 2016, Pages: 31-35
Received: Aug. 8, 2016; Accepted: Aug. 17, 2016; Published: Sep. 5, 2016
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Eman N. Helles, Department of Medical Laboratory Sciences, Faculty of Health Sciences, Islamic University of Gaza, Gaza, Palestine
Mohammed J. Ashour, Department of Medical Laboratory Sciences, Faculty of Health Sciences, Islamic University of Gaza, Gaza, Palestine
Fadel A. Sharif, Department of Medical Laboratory Sciences, Faculty of Health Sciences, Islamic University of Gaza, Gaza, Palestine
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Cytotoxic T lymphocyte associated antigen-4 (CTLA-4) is considered as a negative regulator of T cell activation and its role in maintaining immune tolerance is well established. The present case-control study aimed to investigate the CTLA-4 +49 A/G, -1661 A/G, -318 C/T and -1722 T/C single nucleotide polymorphisms (SNPs) and predisposition to recurrent pregnancy loss (RPL) in Gaza Strip - Palestine. The study was performed on 200 women with a history of two or more pregnancy losses (case group) and 200 control women with at least two live births and without any previous history of abortion. PCR-based restriction fragment length polymorphism (RFLP-PCR) method was used for genotyping CTLA-4 polymorphisms. Study results revealed that there is no significant association between the allele/genotype frequencies of the four investigated CTLA-4 SNPs and RPL. This trend remained true under dominant, co-dominant and recessive models. The A\G genotype of -1661 A\G polymorphism was higher in patients (45%) as compared to controls (39.5%) but without statistical significance. The minor allele frequencies (MAFs) of the CTLA-4 gene polymorphisms in the patient/control group were as follows: +49A>G: 0.22/0.22, -318 C>T: 0.15/0.11, -1661 A>G: 0.30/0.26 and -1722 T>C: 0.08/0.08. The four investigated CTLA-4 polymorphisms do not contribute to the risk of RPL in the study population. Testing other CTLA-4 gene polymorphisms and the level of CTLA-4 expression in RPL patients is recommended.
Recurrent Pregnancy Loss, CTLA-4, Gene Polymorphism, PCR-RFLP
To cite this article
Eman N. Helles, Mohammed J. Ashour, Fadel A. Sharif, CTLA-4 Gene Polymorphism in Women with Idiopathic Recurrent Pregnancy Loss, International Journal of Genetics and Genomics. Vol. 4, No. 4, 2016, pp. 31-35. doi: 10.11648/j.ijgg.20160404.11
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