Genetic Assessment of the Shrub Syncepalum dulcificum (SCHUMACH & THONN.) Daniell in Nigeria Using the Randomly Amplified Polymorphic DNA (RAPD)
International Journal of Genetics and Genomics
Volume 4, Issue 6, December 2016, Pages: 45-50
Received: Dec. 27, 2016; Accepted: Jan. 7, 2017; Published: Jan. 24, 2017
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Authors
Iloh Andrew Chibuzor, Biodiversity and Climate Research Laboratory, Biotechnology Advanced Research Centre (BARC), Sheda Science and Technology Complex, Abuja, Nigeria
Orosun Bukola, Department of Forest Resources Management, University of Ibadan, Ibadan, Oyo State, Nigeria
Akinyele Olukemi Adejoke, Department of Forest Resources Management, University of Ibadan, Ibadan, Oyo State, Nigeria
Onyenekwe Paul Chidozie, Biodiversity and Climate Research Laboratory, Biotechnology Advanced Research Centre (BARC), Sheda Science and Technology Complex, Abuja, Nigeria
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Abstract
In order to advocate for informed management decisions with regards to endangered species, we assessed genetic diversity and genetic structure in 40 individuals of six natural populations of the shrub Syncepalum dulcificum (SCHUMACH & THONN.) DANIELL growing in South Western Nigeria. Twelve (12) Random Amplified Polymorphic DNA (RAPD) primers were tested on total genomic DNA extracted from silica gel dried leaves. Bands were then scored for reproducibility and scoring error calculated. Several genetic diversity parameters were then tested using the POPGENE v1.32 software. Five (5) primers produced 227 reproducible and clear RAPD bands of which 47 were polymorphic (20.7%). The percentage of polymorphic loci (PPI) within populations ranged from 36% to 68%. Nei’s gene diversity among population (Hs) was 0.03, while at species level (Ht) was 0.18. The coefficient of gene differentiation (Gst) among populations was estimated to be 0.83 with a gene flow rate (Nm) of 2.49 showing high genetic diversity within and among populations. The results however indicate a high similarity between the populations as well as close genetic relationship among them. We infer that S. dulcificum in Nigeria does not represent a genetically diverse population and this may be accounted for due the plant’s its breeding system which is mainly autogamous. This study further suggests an in-situ form of conservation be set up as immediate rescue conservation procedure for the plant.
Keywords
DNA Markers, Genetic Diversity, RAPD, Syncepalum dulcificum
To cite this article
Iloh Andrew Chibuzor, Orosun Bukola, Akinyele Olukemi Adejoke, Onyenekwe Paul Chidozie, Genetic Assessment of the Shrub Syncepalum dulcificum (SCHUMACH & THONN.) Daniell in Nigeria Using the Randomly Amplified Polymorphic DNA (RAPD), International Journal of Genetics and Genomics. Vol. 4, No. 6, 2016, pp. 45-50. doi: 10.11648/j.ijgg.20160406.11
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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