Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods
International Journal of Genetics and Genomics
Volume 4, Issue 6, December 2016, Pages: 79-84
Received: Jul. 18, 2016;
Accepted: Jul. 29, 2016;
Published: Mar. 22, 2017
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Biswarup Ghosh, Department of Neuroscience, Vickie & Jack Farber Institute for Neuroscience, Thomas Jefferson University, Philadelphia, USA
Angelo Lepore, Department of Neuroscience, Vickie & Jack Farber Institute for Neuroscience, Thomas Jefferson University, Philadelphia, USA
George Smith, Department of Neuronal Rehabilitation, Shriners Hospitals Pediatric Research Center, Temple University, Philadelphia, USA
Parkinson’s disease (PD) is characterized as a disease of the basal ganglia, with a progressive degeneration of dopaminergic neurons located in the substantia nigra (SN) and projecting to the striatum with subsequently loss of the nigrostriatal circuit. The potential for therapeutic use of cell transplantation for cell replacement has received a great deal of interest. Transplantation with embryonic ventral mesencephalon (VM) is a therapeutic approach for sporadic form of PD. We established unilaterally 6-OHDA lesioned rat model of Parkinson’s disease. Motor behavioral impairment was found compared with normal rat. Embryonic VM tissue was isolated from E 14 (embryonic 14 days) rat brain. We characterized the VM tissue and dissociated cultured dopaminergic cells with tyrosine hydroxylase (TH) immune staining before transplantation in lesioned brain. We observed that the axons of dopaminergic neurons from transplanted VM graft circles round at the site of transplantation in normal adult rat brain. In this paper, we discuss the detailed methodologies which are very useful in preclinical research of cell based therapies for Parkinson’s disease.
Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods, International Journal of Genetics and Genomics.
Vol. 4, No. 6,
2016, pp. 79-84.
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