Lymphocyte Reproductive Activity Normalized to Numbers of Hematopoietic Stem Cells in Blood and Rate of Death in Fatal Diseases
International Journal of Genetics and Genomics
Volume 5, Issue 5, October 2017, Pages: 54-62
Received: Jan. 29, 2017; Accepted: Feb. 13, 2017; Published: Oct. 18, 2017
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Aleksei N. Shoutko, Laboratory for Improvement of the Treatment Methods, Federal Research Center for Radiology and Surgical Technologies, Saint-Petersburg, Russia
Olga A. Gerasimova, Transplantation Division, Federal Research Center for Radiology and Surgical Technologies, Saint-Petersburg, Russia
Ludmila P. Ekimova, Laboratory for Improvement of the Treatment Methods, Federal Research Center for Radiology and Surgical Technologies, Saint-Petersburg, Russia
Fiodor Zherebtsov, Transplantation Division, Federal Research Center for Radiology and Surgical Technologies, Saint-Petersburg, Russia
Viktor F. Mus, Group for Lung Cancer Treatment, Federal Research Center for Radiology and Surgical Technologies, Saint-Petersburg, Russia
Kirill S. Matyurin, Laboratory for Improvement of the Treatment Methods, Federal Research Center for Radiology and Surgical Technologies, Saint-Petersburg, Russia
Anatoly M. Granov, Transplantation Division, Federal Research Center for Radiology and Surgical Technologies, Saint-Petersburg, Russia
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The numbers of CD133+ and CD31+ lymphocytes and those in the G2-M phases in the total fraction of circulating lymphocytes from patients with fatal liver cirrhosis and advanced lung cancer were investigated by flow cytometry during a long period of conventional treatment with OLT or palliative surgery followed by myelosuppressive chemotherapy. The relationships of specific reproductive activity, sRA (G2-M/CD133+), and the number of committed liver α-fetoprotein-positive (AFP+) cells with the rate of patient deaths, characterized by exponential approximation survival curves for both diseases, were investigated. Subnormal sRA in patients after OLT and excessive sRA in LC patients above a healthy level were associated with higher death rates and lower survival, coinciding with strong immunosuppression caused by anti-rejection and anti-cancer therapies. These findings may be explained by morphogenesis (feeding) activity of circulating lymphocytes targeted toward both normal and malignant tissues rather than in terms of cellular immunity. The sRA changes may be a useful indicator for monitoring the potential for engraftment or tumor growth.
Hematopoietic Stem Cells, Lymphocytes, Specific Reproductive Activity, Resource of Lymphopoiesis, Lung Cancer Therapy, Liver Transplantation, Death Rate
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Aleksei N. Shoutko, Olga A. Gerasimova, Ludmila P. Ekimova, Fiodor Zherebtsov, Viktor F. Mus, Kirill S. Matyurin, Anatoly M. Granov, Lymphocyte Reproductive Activity Normalized to Numbers of Hematopoietic Stem Cells in Blood and Rate of Death in Fatal Diseases, International Journal of Genetics and Genomics. Vol. 5, No. 5, 2017, pp. 54-62. doi: 10.11648/j.ijgg.20170505.12
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