Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt

Hanaa Mahdy Abouzied; Walid Mohamed El-Orabey; Mohamed Abd El-Halim Abou-Zeid

doi:doi:10.11648/j.ijgg.20180604.11

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Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt

Hanaa Mahdy Abouzied, Walid Mohamed El-Orabey, Mohamed Abd El-Halim Abou-Zeid

Published in International Journal of Genetics and Genomics (Volume 6, Issue 4)

Received: 12 November 2018 Accepted: 4 December 2018 Published: 23 January 2019

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Abstract

Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. A mapping population of 204 RILs (F6:8) obtained from the cross between two parents of winter wheat ‘Harry’ x ‘Wesley’ through single seed decent method was used to identify QTL region associated with leaf rust resistance genes in wheat. Under the Green house condition in Egypt leaf rust pathotypes i.e NTTJT, PTTGS, PTTTT, TTTBT and TTTTT were used. High-density linkage map based on GBS derived SNPs were applied in this study for QTL mapping. The wheat genotype Harry was resistant to all tested pathotype while, the wheat genotype Wesley was susceptible to all tested pathotype. One major stable QTLs, for race NTTJT was identified on chromosome 6A flanked by markers XSNP3958 and XSNP3957 with a LOD 4.22. The identified SNP marker may be used to screen for resistance to specific races of leaf rust found in Egypt in further spring wheat breeding programs as marker assisted selection.

Published in	International Journal of Genetics and Genomics (Volume 6, Issue 4)
DOI	10.11648/j.ijgg.20180604.11
Page(s)	37-43
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Wheat, Leaf Rust, QTL Mapping, GBS by Sequencing

References

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APA Style

Hanaa Mahdy Abouzied, Walid Mohamed El-Orabey, Mohamed Abd El-Halim Abou-Zeid. (2019). Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt. International Journal of Genetics and Genomics, 6(4), 37-43. https://doi.org/10.11648/j.ijgg.20180604.11

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ACS Style

Hanaa Mahdy Abouzied; Walid Mohamed El-Orabey; Mohamed Abd El-Halim Abou-Zeid. Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt. Int. J. Genet. Genomics 2019, 6(4), 37-43. doi: 10.11648/j.ijgg.20180604.11

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AMA Style

Hanaa Mahdy Abouzied, Walid Mohamed El-Orabey, Mohamed Abd El-Halim Abou-Zeid. Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt. Int J Genet Genomics. 2019;6(4):37-43. doi: 10.11648/j.ijgg.20180604.11

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@article{10.11648/j.ijgg.20180604.11,
  author = {Hanaa Mahdy Abouzied and Walid Mohamed El-Orabey and Mohamed Abd El-Halim Abou-Zeid},
  title = {Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt},
  journal = {International Journal of Genetics and Genomics},
  volume = {6},
  number = {4},
  pages = {37-43},
  doi = {10.11648/j.ijgg.20180604.11},
  url = {https://doi.org/10.11648/j.ijgg.20180604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20180604.11},
  abstract = {Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. A mapping population of 204 RILs (F6:8) obtained from the cross between two parents of winter wheat ‘Harry’ x ‘Wesley’ through single seed decent method was used to identify QTL region associated with leaf rust resistance genes in wheat. Under the Green house condition in Egypt leaf rust pathotypes i.e NTTJT, PTTGS, PTTTT, TTTBT and TTTTT were used. High-density linkage map based on GBS derived SNPs were applied in this study for QTL mapping. The wheat genotype Harry was resistant to all tested pathotype while, the wheat genotype Wesley was susceptible to all tested pathotype. One major stable QTLs, for race NTTJT was identified on chromosome 6A flanked by markers XSNP3958 and XSNP3957 with a LOD 4.22. The identified SNP marker may be used to screen for resistance to specific races of leaf rust found in Egypt in further spring wheat breeding programs as marker assisted selection.},
 year = {2019}
}

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TY  - JOUR
T1  - Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt
AU  - Hanaa Mahdy Abouzied
AU  - Walid Mohamed El-Orabey
AU  - Mohamed Abd El-Halim Abou-Zeid
Y1  - 2019/01/23
PY  - 2019
N1  - https://doi.org/10.11648/j.ijgg.20180604.11
DO  - 10.11648/j.ijgg.20180604.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 37
EP  - 43
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20180604.11
AB  - Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. A mapping population of 204 RILs (F6:8) obtained from the cross between two parents of winter wheat ‘Harry’ x ‘Wesley’ through single seed decent method was used to identify QTL region associated with leaf rust resistance genes in wheat. Under the Green house condition in Egypt leaf rust pathotypes i.e NTTJT, PTTGS, PTTTT, TTTBT and TTTTT were used. High-density linkage map based on GBS derived SNPs were applied in this study for QTL mapping. The wheat genotype Harry was resistant to all tested pathotype while, the wheat genotype Wesley was susceptible to all tested pathotype. One major stable QTLs, for race NTTJT was identified on chromosome 6A flanked by markers XSNP3958 and XSNP3957 with a LOD 4.22. The identified SNP marker may be used to screen for resistance to specific races of leaf rust found in Egypt in further spring wheat breeding programs as marker assisted selection.
VL  - 6
IS  - 4
ER  -

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Author Information

Hanaa Mahdy Abouzied

Department of Crop Science, Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Walid Mohamed El-Orabey

Department of Wheat Diseases Research, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Mohamed Abd El-Halim Abou-Zeid

Department of Wheat Diseases Research, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

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APA Style

Hanaa Mahdy Abouzied, Walid Mohamed El-Orabey, Mohamed Abd El-Halim Abou-Zeid. (2019). Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt. International Journal of Genetics and Genomics, 6(4), 37-43. https://doi.org/10.11648/j.ijgg.20180604.11

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ACS Style

Hanaa Mahdy Abouzied; Walid Mohamed El-Orabey; Mohamed Abd El-Halim Abou-Zeid. Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt. Int. J. Genet. Genomics 2019, 6(4), 37-43. doi: 10.11648/j.ijgg.20180604.11

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AMA Style

Hanaa Mahdy Abouzied, Walid Mohamed El-Orabey, Mohamed Abd El-Halim Abou-Zeid. Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt. Int J Genet Genomics. 2019;6(4):37-43. doi: 10.11648/j.ijgg.20180604.11

Copy | Download

@article{10.11648/j.ijgg.20180604.11,
  author = {Hanaa Mahdy Abouzied and Walid Mohamed El-Orabey and Mohamed Abd El-Halim Abou-Zeid},
  title = {Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt},
  journal = {International Journal of Genetics and Genomics},
  volume = {6},
  number = {4},
  pages = {37-43},
  doi = {10.11648/j.ijgg.20180604.11},
  url = {https://doi.org/10.11648/j.ijgg.20180604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20180604.11},
  abstract = {Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. A mapping population of 204 RILs (F6:8) obtained from the cross between two parents of winter wheat ‘Harry’ x ‘Wesley’ through single seed decent method was used to identify QTL region associated with leaf rust resistance genes in wheat. Under the Green house condition in Egypt leaf rust pathotypes i.e NTTJT, PTTGS, PTTTT, TTTBT and TTTTT were used. High-density linkage map based on GBS derived SNPs were applied in this study for QTL mapping. The wheat genotype Harry was resistant to all tested pathotype while, the wheat genotype Wesley was susceptible to all tested pathotype. One major stable QTLs, for race NTTJT was identified on chromosome 6A flanked by markers XSNP3958 and XSNP3957 with a LOD 4.22. The identified SNP marker may be used to screen for resistance to specific races of leaf rust found in Egypt in further spring wheat breeding programs as marker assisted selection.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt
AU  - Hanaa Mahdy Abouzied
AU  - Walid Mohamed El-Orabey
AU  - Mohamed Abd El-Halim Abou-Zeid
Y1  - 2019/01/23
PY  - 2019
N1  - https://doi.org/10.11648/j.ijgg.20180604.11
DO  - 10.11648/j.ijgg.20180604.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 37
EP  - 43
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20180604.11
AB  - Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. A mapping population of 204 RILs (F6:8) obtained from the cross between two parents of winter wheat ‘Harry’ x ‘Wesley’ through single seed decent method was used to identify QTL region associated with leaf rust resistance genes in wheat. Under the Green house condition in Egypt leaf rust pathotypes i.e NTTJT, PTTGS, PTTTT, TTTBT and TTTTT were used. High-density linkage map based on GBS derived SNPs were applied in this study for QTL mapping. The wheat genotype Harry was resistant to all tested pathotype while, the wheat genotype Wesley was susceptible to all tested pathotype. One major stable QTLs, for race NTTJT was identified on chromosome 6A flanked by markers XSNP3958 and XSNP3957 with a LOD 4.22. The identified SNP marker may be used to screen for resistance to specific races of leaf rust found in Egypt in further spring wheat breeding programs as marker assisted selection.
VL  - 6
IS  - 4
ER  -

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