Amaranthus hybridus L. is an important vegetable in Nigeria, grown mainly for its highly nutritious leaves. In a bid to explore the possibility of increasing the infra-specific diversity of the crop, we induced three accessions of A. hybridus from Anambra State, Nigeria with x-ray particles using various concentration levels. The experimental layout composed a 5 x 3 x 3 Randomized Complete Block Design arrangement, with treatment levels: 1 MGY, 2 MGY, 4 MGY, and 6 MGY, selectively allotted to the different accession based on a preliminary dosimetry test. Morphological data were recorded and subsequently leaves samples subjected to Deoxyribonucleic Acid (DNA) extraction and Random Amplified Polymorphic DNA (RAPD) analysis. The results show that except for inflorescence weight, other morphological data were not statistically significant. However, RAPD techniques identify some variations induced by x-ray particles on the different accessions. Ogbunike accession irradiated with 4 MGY appears to show the optimum concentration of x-ray particles required to induce genetic variability in A. hybridus. These findings are of paramount importance to plant breeders, whose primary tool is to identify variations to produce better yielding varieties.
| Published in | International Journal of Genetics and Genomics (Volume 7, Issue 2) |
| DOI | 10.11648/j.ijgg.20190702.11 |
| Page(s) | 18-26 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
X-Ray, Amaranthus hybridus L., Genetic Variability, Mutation, Diversity, RAPD
| [1] | Templeton, A. R. (1995). Biodiversity at the molecular genetic level: experiences from disparate macroorganisms. In: Hawksworth D. L. (ed). Biodiversity. Chapman and Hall, London, pp. 59–64. |
| [2] | Frankham, R. (2005). Genetics and extinction. Biological Conservation. 126 (2): 131–140. |
| [3] | Bhandari, H. R., Bhanu, A. H., Srivastava, K., Singh, M. N; Hemantaranjan, S. A. (2017). Assessment of genetic diversity in crop plants- an overview. Advances in Plants & Agriculture Research, 7 (3): 279-286. |
| [4] | Garrett, R. H. and Grisham, C. M. (2013). Biochemistry. Brook Scole Publishers, United Kingdom, pp 122. |
| [5] | Sen, S. and Kar, D. K. (2009). Cytology and Genetics. Alpha Science International Ltd., UK, pp 404. |
| [6] | Schreiber, M. Stein, N and Masch, M. (2018). Genomic approaches for studying crop evolution. Genome Biology, 19: 140. |
| [7] | Snezana, D. M., Marija, K., Danijela, R., Milena, S. and Lidija, S. (2012). Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and Random Amplified Polymorphic DNA (RAPD) Markers. African Journal of Biotechnology, 11 (29): 7331-7337. |
| [8] | Park, Y. and Nishikawa, T. (2012). Rapid identification of Amaranthus caudatus and Amaranthus hypochondriacus by sequencing and PCR-RFLP analysis of two starch synthase genes. Genome, 55: 623-628. |
| [9] | Akin-Idowu, P. E., Odunola, O. A., Gbadegesin, M. A., Oke, A. and Orkpeh, U. (2013). Assessment of the protein quality of twenty nine grain amaranth (Amaranthus spp. L.) accession using amino acid analysis and one-dimensional electrophoresis. African Journal of Biotechnology, 12 (15): 1802-1810. |
| [10] | Muyonga, J. H., Nabakabya, D., Nakimbugwe, D. N., and Masinde, D. (2008). Efforts to promote amaranth production and consumption in Uganda to fight malnutrition. In: Using Food Science and Technology to Improve Nutrition and Promote National Development. Robertson, G. L. and Lupien, J. R. (eds.). International Union of Food Science and Technology, Vol. 1, pp. 40-42. |
| [11] | Brenner, D., Baltensperger, D., Kulakow, P., Lehmann, J., Myers, R., Slabbert, M., and Sleugh, B. (2000). Genetic resources and breeding of Amaranthus. Plant Breeding Revolution, 19: 227-285. |
| [12] | Yudina, R. S., Zheleznova, N. B., Zakharova, O. V., Zhelenov, A. V., and Shumny, V. K. (2005). Isosyme analysis in a genetic collection of amaranths (Amaranthus L.). Russian Journal of Genetics, 41 (12): 1395-1400. |
| [13] | Hricova, A., Fejer, J., Libiakova, G. and Gajdosova, A. (2012). Adding value to amaranth through the use of radiation mutagenesis. Neglected and Under-Utilized Species Research in 21st Century, pp. 15. |
| [14] | Schoelenchner, R. and Tomoskozi, S. (2012). Use of speciality and underutilized grain species and pseudocereals for gluten-free food production. Neglected and Under-Utilized Species Research in 21st Century, pp. 42-43. |
| [15] | Gudu, S., and Gupta, V. K. (1988). Electophoresis as an aid for identification of various species and cultivars of grains amaranths. Acta Horticult., 218: 230–238. |
| [16] | Gorinstein, S., De Nue, I. A., and Aruda, P. (1991). Alcohol soluble and total proteins from Amaranthus seeds and their comparison with other cereals. Journal of Agriculture, Food and Chemistry, 39: 848–850. |
| [17] | Zheleznov, A. V., Solonenko, L. P., and Zheleznova, N. B. (1997). Seed protein of the wild and cultivated Amarathus species. Euphytica, 97: 177-182. |
| [18] | Drzewiecki, J. (2001). Similarities and differences between Amaranthus species and cultivars and estimation of out crossing rate on the basis of electrophoretic separations of urea– soluble seed proteins. Euphytica, 119 (3): 279–287. |
| [19] | Juan, R., Pastor, J., Alaiz, M. and Vioque, J. R. (2007). Electrophoretic characterization of Amaranthus L. seed proteins and its systematic implication. Botany Journal of Linnaeus Society, 155 (1): 57–63. |
| [20] | Keckesova, M., Mudry, P., Hricova, A., and Galova, Z. (2012). Enzyme polymorphic analysis in irradiated amaranth (Amaranthus spp.). Neglected and Under-Utilized Species Research in 21st Century, pp. 6-9. |
| [21] | De Clemente, S. (2012). X-ray radiation effects on germination and growth of Echinacea augustifolia. Http://www.google.com. [Accessed on January 6, 2016]. |
| [22] | Russell, M. A. (1937). Effect of x-ray on Zea mays. WWW.Plantphysiol.org. [Accessed on January 6, 2016]. |
| [23] | El-mokadem, H. E. and Mostafa, G. G. (2014). Induction of mutations in Browallia speciosa using sodium azide and identification of the genetic variation by peroxidase isozyme. African Journal of Biotechnology, 13 (1): 106–111. |
| [24] | El- Nasher, Y. I. A. 2006. Effect of chemical mutagens (sodium azide and diethyl sulphate) on growth, flowering and induced variability in Amaranthus caudatus L. and A. hypochondriacus L. Ph. D thesis, Alexandria University, p. 80. |
| [25] | Mostafa, G. G. (2011). Effect of sodium azide on the growth and variability induction in Helianthus annus L. International Journal of Plant Breeding and genetics, 5 (1): 76–85 |
| [26] | Guillermo C. A. Vidal-Russel R., Aizen M. A., Nickrent D. (2014). Genetic diversity and population structure of the mistotle. Plant Systematics Evolution, 300: 153-162. |
| [27] | Jimoh, M. A., Edeoga, H. O. and Omosun, G. (2013). DNA fingerprinting of six Senna Species and the taxonomic implication. International Journal of Advanced Research in Biotechnology, 1 (6): 022-026. |
| [28] | Hadrys, H., Balick, M., and Schierwater, B. (1992). Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Molecular Ecology, 1: 53-63. |
| [29] | Williams, J. G. K., Hanafey, M. K., Rafalski J. A., and Tingey, S. V. (1993). Genetic analysis using random amplified polymorphic DNA markers methods. Enzymology, 218: 704-740. |
| [30] | Das, M., Bhaitacharya, S., and Pal, A. (2005). Generation and characterization of SCARS by cloning and sequencing of RAPD products: a strategy for specific marker development in bamboo. Annals of Botany, 95: 835-841. |
| [31] | [31] Rahman, M. O. (2007). DNA fingerprinting in Utricularia L. section Ultricularia as revealed by PCR based assay. International Journal of Botany, 3 (1): 56-68. |
| [32] | Anonymous (2016). Procedure for mutation breeding. Http://www.google.com. Accessed on January 6, 2016. |
| [33] | Mba, C. (2013). Induced mutations unleash the potentials of plant genetic resources for Food and Agriculture. Agronomy, 3: 200-231. |
| [34] | Gomez-Pando, L. (2014). Development of improved varieties of native grains through radiation-induced mutagenesis. In: Mutagenesis: exploring novel genes and pathways. N. B. Tomlekova, M. I. Kozgar and M. R. Wani (eds.). Wageningen Academic Publishers, Wageningen, 105-123 Pages. |
| [35] | Doyle, J. J. and Doyle, J. L. (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15. |
| [36] | Schum, A. (2003). Mutation breeding in ornamental and efficient breeding method. Acta Horticulture, 612: 47-60. |
| [37] | Song, H. S. and Kang, S. Y. (2003). Application of natural variation and induced mutation in breeding and functional genomics: papers for international symposium: current status and future of plant mutation breeding. Korea Journal of Breeding Science, 35 (1): 24-34. |
| [38] | Yoon, K. E., Park, Y. H. and Im, B. G. (1990). Effect of gamma radiation on seed germination and androgenesis in Nicotiana tabacum L. Korean Journal of Breeding Science, 21: 256-262. |
| [39] | Shin, J., Kim, B., Seo, S., Jeon, S. B., Kim, J., Jun, B., Kang, S., Lee, J., Chung, M. and Kim, S. (2011). Mutation breeding of sweet potato by gamma-ray radiation. African Journal of Agricultural Research, 6 (6): 1447-1454. |
APA Style
Beluchukwu Joseph Nwankwo, Garuba Omosun, Hilary Odo Edeoga, Odoligie Imarhiagbe, Jeffrey Iyare Omoruyi, et al. (2019). X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data. International Journal of Genetics and Genomics, 7(2), 18-26. https://doi.org/10.11648/j.ijgg.20190702.11
ACS Style
Beluchukwu Joseph Nwankwo; Garuba Omosun; Hilary Odo Edeoga; Odoligie Imarhiagbe; Jeffrey Iyare Omoruyi, et al. X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data. Int. J. Genet. Genomics 2019, 7(2), 18-26. doi: 10.11648/j.ijgg.20190702.11
AMA Style
Beluchukwu Joseph Nwankwo, Garuba Omosun, Hilary Odo Edeoga, Odoligie Imarhiagbe, Jeffrey Iyare Omoruyi, et al. X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data. Int J Genet Genomics. 2019;7(2):18-26. doi: 10.11648/j.ijgg.20190702.11
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Download@article{10.11648/j.ijgg.20190702.11,
author = {Beluchukwu Joseph Nwankwo and Garuba Omosun and Hilary Odo Edeoga and Odoligie Imarhiagbe and Jeffrey Iyare Omoruyi and Edeji Franklin Uzodinma},
title = {X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data},
journal = {International Journal of Genetics and Genomics},
volume = {7},
number = {2},
pages = {18-26},
doi = {10.11648/j.ijgg.20190702.11},
url = {https://doi.org/10.11648/j.ijgg.20190702.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20190702.11},
abstract = {Amaranthus hybridus L. is an important vegetable in Nigeria, grown mainly for its highly nutritious leaves. In a bid to explore the possibility of increasing the infra-specific diversity of the crop, we induced three accessions of A. hybridus from Anambra State, Nigeria with x-ray particles using various concentration levels. The experimental layout composed a 5 x 3 x 3 Randomized Complete Block Design arrangement, with treatment levels: 1 MGY, 2 MGY, 4 MGY, and 6 MGY, selectively allotted to the different accession based on a preliminary dosimetry test. Morphological data were recorded and subsequently leaves samples subjected to Deoxyribonucleic Acid (DNA) extraction and Random Amplified Polymorphic DNA (RAPD) analysis. The results show that except for inflorescence weight, other morphological data were not statistically significant. However, RAPD techniques identify some variations induced by x-ray particles on the different accessions. Ogbunike accession irradiated with 4 MGY appears to show the optimum concentration of x-ray particles required to induce genetic variability in A. hybridus. These findings are of paramount importance to plant breeders, whose primary tool is to identify variations to produce better yielding varieties.},
year = {2019}
}
TY - JOUR T1 - X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data AU - Beluchukwu Joseph Nwankwo AU - Garuba Omosun AU - Hilary Odo Edeoga AU - Odoligie Imarhiagbe AU - Jeffrey Iyare Omoruyi AU - Edeji Franklin Uzodinma Y1 - 2019/06/26 PY - 2019 N1 - https://doi.org/10.11648/j.ijgg.20190702.11 DO - 10.11648/j.ijgg.20190702.11 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 18 EP - 26 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20190702.11 AB - Amaranthus hybridus L. is an important vegetable in Nigeria, grown mainly for its highly nutritious leaves. In a bid to explore the possibility of increasing the infra-specific diversity of the crop, we induced three accessions of A. hybridus from Anambra State, Nigeria with x-ray particles using various concentration levels. The experimental layout composed a 5 x 3 x 3 Randomized Complete Block Design arrangement, with treatment levels: 1 MGY, 2 MGY, 4 MGY, and 6 MGY, selectively allotted to the different accession based on a preliminary dosimetry test. Morphological data were recorded and subsequently leaves samples subjected to Deoxyribonucleic Acid (DNA) extraction and Random Amplified Polymorphic DNA (RAPD) analysis. The results show that except for inflorescence weight, other morphological data were not statistically significant. However, RAPD techniques identify some variations induced by x-ray particles on the different accessions. Ogbunike accession irradiated with 4 MGY appears to show the optimum concentration of x-ray particles required to induce genetic variability in A. hybridus. These findings are of paramount importance to plant breeders, whose primary tool is to identify variations to produce better yielding varieties. VL - 7 IS - 2 ER -
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