International Journal of Genetics and Genomics

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Prediction of the Structure and Mutations Instability of the Med12 Exon2 Gene in Uterine Fibroids in Senegalese Women

Received: 09 September 2019    Accepted: 20 September 2019    Published: 30 September 2019
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Abstract

Uterine fibroids are benign proliferations of slow evolution. They are associated with significant morbidity and constitute a real public health problem. Despite the large-scale medical and financial burden posed by uterine fibroids, the functional roles of the various factors and genes involved in their etiology and growth remain unclear. This shows a great need to undertake a study that would evaluate the molecular features of uterine fibroids. It is in this context that our study is based on 50 Senegalese women with uterine fibroids. Samples of tumour tissue and blood were taken from each patient. After sequencing, the raw data was submitted to the Mutation Surveyor software version 5.0.1. Pathogenicity of mutations was evaluated with Polyphen software. To better understand the functional impact of missense mutations at the three-dimensional level, we simulated the structure of the protein by the ab-initio method using the I-Tasser web server. After cleaning, correcting and aligning the sequences with the BioEdit software version 8.0.5, the amino acid frequencies for the blood and tumour tissue samples were retrieved with the MEGA7 software. To see if there is a difference in the distribution of each amino acid between blood and tumour tissue, the average comparison test was performed with the R software version 3.3.1. Our results showed the presence of mutations of the MED12 gene only in tumour tissues. All mutations are predicted to be deleterious. In comparison to the reference sequence, all the mutations show a conformational change in the 3D structure of the MED12 protein. In addition, the mutations p. Q43P, p. G44S, p. G44D, p. G44R, p. F45V, p. K60M give proteins of α-β structure different from the reference sequence which has an α structure. All mutations alter the predicted function of the MED12 protein, which further suggests their involvement in the pathobiology of uterine fibroids in Senegalese women. With regard to amino acid frequencies, the comparison of means between blood and tumour tissue samples shows different distributions for amino acids such as cysteine, aspartic acid, glycine, histidine, leucine, arginine and serine. The results obtained make it possible to better understand the molecular mechanisms involved in the etiology of uterine fibroids. They allow glimpsing applications for the screening of populations at risk, for a non-invasive diagnosis or even for preventive or curative treatment.

DOI 10.11648/j.ijgg.20190703.18
Published in International Journal of Genetics and Genomics (Volume 7, Issue 3, September 2019)
Page(s) 80-87
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Uterine Fibroids, MED12 Mutations, Amino Acids, Biomarker

References
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Author Information
  • Department of Animal Biology, Cheikh Anta Diop University, Dakar, Senegal; Genetics and Population Management Team, Dakar, Senegal

  • Maternity and Gynecology Obstetrics Service, Grand Yoff Hospital, Dakar, Senegal

  • Joliot Curie Institute, Le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal

  • Joliot Curie Institute, Le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal

  • Department of Animal Biology, Cheikh Anta Diop University, Dakar, Senegal; Genetics and Population Management Team, Dakar, Senegal

  • Urology Department, General Hospital Grand Yoff, Dakar, Senegal

Cite This Article
  • APA Style

    Keneme Bineta, Ciss Daouda, Ka Sidy, Dem Ahmadou, Sembene Pape Mbacke, et al. (2019). Prediction of the Structure and Mutations Instability of the Med12 Exon2 Gene in Uterine Fibroids in Senegalese Women. International Journal of Genetics and Genomics, 7(3), 80-87. https://doi.org/10.11648/j.ijgg.20190703.18

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    ACS Style

    Keneme Bineta; Ciss Daouda; Ka Sidy; Dem Ahmadou; Sembene Pape Mbacke, et al. Prediction of the Structure and Mutations Instability of the Med12 Exon2 Gene in Uterine Fibroids in Senegalese Women. Int. J. Genet. Genomics 2019, 7(3), 80-87. doi: 10.11648/j.ijgg.20190703.18

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    AMA Style

    Keneme Bineta, Ciss Daouda, Ka Sidy, Dem Ahmadou, Sembene Pape Mbacke, et al. Prediction of the Structure and Mutations Instability of the Med12 Exon2 Gene in Uterine Fibroids in Senegalese Women. Int J Genet Genomics. 2019;7(3):80-87. doi: 10.11648/j.ijgg.20190703.18

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  • @article{10.11648/j.ijgg.20190703.18,
      author = {Keneme Bineta and Ciss Daouda and Ka Sidy and Dem Ahmadou and Sembene Pape Mbacke and Serigne Magueye Gueye},
      title = {Prediction of the Structure and Mutations Instability of the Med12 Exon2 Gene in Uterine Fibroids in Senegalese Women},
      journal = {International Journal of Genetics and Genomics},
      volume = {7},
      number = {3},
      pages = {80-87},
      doi = {10.11648/j.ijgg.20190703.18},
      url = {https://doi.org/10.11648/j.ijgg.20190703.18},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijgg.20190703.18},
      abstract = {Uterine fibroids are benign proliferations of slow evolution. They are associated with significant morbidity and constitute a real public health problem. Despite the large-scale medical and financial burden posed by uterine fibroids, the functional roles of the various factors and genes involved in their etiology and growth remain unclear. This shows a great need to undertake a study that would evaluate the molecular features of uterine fibroids. It is in this context that our study is based on 50 Senegalese women with uterine fibroids. Samples of tumour tissue and blood were taken from each patient. After sequencing, the raw data was submitted to the Mutation Surveyor software version 5.0.1. Pathogenicity of mutations was evaluated with Polyphen software. To better understand the functional impact of missense mutations at the three-dimensional level, we simulated the structure of the protein by the ab-initio method using the I-Tasser web server. After cleaning, correcting and aligning the sequences with the BioEdit software version 8.0.5, the amino acid frequencies for the blood and tumour tissue samples were retrieved with the MEGA7 software. To see if there is a difference in the distribution of each amino acid between blood and tumour tissue, the average comparison test was performed with the R software version 3.3.1. Our results showed the presence of mutations of the MED12 gene only in tumour tissues. All mutations are predicted to be deleterious. In comparison to the reference sequence, all the mutations show a conformational change in the 3D structure of the MED12 protein. In addition, the mutations p. Q43P, p. G44S, p. G44D, p. G44R, p. F45V, p. K60M give proteins of α-β structure different from the reference sequence which has an α structure. All mutations alter the predicted function of the MED12 protein, which further suggests their involvement in the pathobiology of uterine fibroids in Senegalese women. With regard to amino acid frequencies, the comparison of means between blood and tumour tissue samples shows different distributions for amino acids such as cysteine, aspartic acid, glycine, histidine, leucine, arginine and serine. The results obtained make it possible to better understand the molecular mechanisms involved in the etiology of uterine fibroids. They allow glimpsing applications for the screening of populations at risk, for a non-invasive diagnosis or even for preventive or curative treatment.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Prediction of the Structure and Mutations Instability of the Med12 Exon2 Gene in Uterine Fibroids in Senegalese Women
    AU  - Keneme Bineta
    AU  - Ciss Daouda
    AU  - Ka Sidy
    AU  - Dem Ahmadou
    AU  - Sembene Pape Mbacke
    AU  - Serigne Magueye Gueye
    Y1  - 2019/09/30
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijgg.20190703.18
    DO  - 10.11648/j.ijgg.20190703.18
    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
    SP  - 80
    EP  - 87
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20190703.18
    AB  - Uterine fibroids are benign proliferations of slow evolution. They are associated with significant morbidity and constitute a real public health problem. Despite the large-scale medical and financial burden posed by uterine fibroids, the functional roles of the various factors and genes involved in their etiology and growth remain unclear. This shows a great need to undertake a study that would evaluate the molecular features of uterine fibroids. It is in this context that our study is based on 50 Senegalese women with uterine fibroids. Samples of tumour tissue and blood were taken from each patient. After sequencing, the raw data was submitted to the Mutation Surveyor software version 5.0.1. Pathogenicity of mutations was evaluated with Polyphen software. To better understand the functional impact of missense mutations at the three-dimensional level, we simulated the structure of the protein by the ab-initio method using the I-Tasser web server. After cleaning, correcting and aligning the sequences with the BioEdit software version 8.0.5, the amino acid frequencies for the blood and tumour tissue samples were retrieved with the MEGA7 software. To see if there is a difference in the distribution of each amino acid between blood and tumour tissue, the average comparison test was performed with the R software version 3.3.1. Our results showed the presence of mutations of the MED12 gene only in tumour tissues. All mutations are predicted to be deleterious. In comparison to the reference sequence, all the mutations show a conformational change in the 3D structure of the MED12 protein. In addition, the mutations p. Q43P, p. G44S, p. G44D, p. G44R, p. F45V, p. K60M give proteins of α-β structure different from the reference sequence which has an α structure. All mutations alter the predicted function of the MED12 protein, which further suggests their involvement in the pathobiology of uterine fibroids in Senegalese women. With regard to amino acid frequencies, the comparison of means between blood and tumour tissue samples shows different distributions for amino acids such as cysteine, aspartic acid, glycine, histidine, leucine, arginine and serine. The results obtained make it possible to better understand the molecular mechanisms involved in the etiology of uterine fibroids. They allow glimpsing applications for the screening of populations at risk, for a non-invasive diagnosis or even for preventive or curative treatment.
    VL  - 7
    IS  - 3
    ER  - 

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