International Journal of Genetics and Genomics

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In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20

Received: 21 July 2014    Accepted: 07 August 2014    Published: 20 August 2014
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Abstract

Background: Globally, respiratory diseases cause an estimated 1.9 million deaths per year. One of the most important aetiological organisms of both adult and childhood respiratory disease is Non-Typeable Haemophilus influenzae (NTHi). NTHi is frequently isolated from the respiratory tract during episodes of sinusitis, Otitis Media and pneumonia and is the most common cause of Chronic Obstructive Pulmonary Disease (COPD) and bronchiectasis exacerbations. Methods: The work has been effectively complemented with the compilation of the Database of Essential Genes (DEG) of H. influenzae Rd KW20. Each protein is subjected to BLASTP in NCBI server http://www.ncbi.nlm.nih.gov/blastp. The candidate drug targets are determined by KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES. Results: The given gram negative bacteria H. influenzae Rd KW20 has six distinguished domains i.e., cytoplasmic, cytoplasmic membrane, periplasmic, outer membrane, extracellular and unknown domains. Out of 642 essential genes, the predicted non-human Homologous are 412 in the different domain of given bacteria. With the help of KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES, we successfully identified 35 novel drug targets which have common metabolic pathways both in host and pathogen & pathogen specific metabolic pathways. Conclusion: The novel drug targets suggest those genes which are active in both the host and pathogen metabolic pathway and should be a pathogen specific metabolic pathway. The important drug target regions are vacJ, lepB, emrB, MurG & dgkA. vacJ is present in outer membrane while lepB, emrB, MurG&dgkA are present in cytoplasmic membrane. All these genes are fully sequenced and specifically annotated in KEGG PATHWAY.

DOI 10.11648/j.ijgg.20140204.13
Published in International Journal of Genetics and Genomics (Volume 2, Issue 4, August 2014)
Page(s) 62-67
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

NTHi (Non-Typeable H. Influenzae), NP (Non-Typeable) , DEG (Database of Essential Genes), Rd (Rough Derivative), ChoP (Phosphorylcholine), COPD (Chronic Obstructive Pulmonary Disease), SVM (Support Vector Machine), SCL (Subcellular Localization)

References
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[4] Van SMH, Van LA, Mooi FR, Van JP. Contribution of the major and minor subunits to fimbria-Mediated adherence of H. influenzae to human epithelial cells and erythrocytes. Infect Immun 1995; 63: 4883-9.
[5] Hendrixson DR, Geme III JWS. Haemophilus influenzae Hap serine protease promotes adherence and microcolony formation, potentiated by a soluble host protein. Mol Cell 1998; 2: 841-50.
[6] Murley YM, Edlind TD, Plett PA, LiPuma JJ. Cloning of the haemocin locus of H. influenzae type b and assessment of the role of Haemocin in virulence. Microbiology 1998; 144:2531-8.
[7] Duim B, Vogel L, Puijk W, Jansen HM, Meloen RH, Dankert J, et. al. Fine mapping of outer membrane protein P2 antigenic sites which vary 55 during persistent infection by Haemophilus influenzae. Infection and immunity 1996; 64:4673-79.
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[9] Prymula R, Peeters P, Chrobok V, Kriz P, Novakova E, Kaliskova E, et al. Pneumococcal capsular polysaccharides conjugated to protein D for prevention of acute Otitis Media caused by both Streptococcus pneumonia and non-typable H. influenzae: a randomised double-blind efficacy study. Lancet 2006; 367:740-8.annotation and pathway reconstruction server. Nucleic Acids Research 2007; 35:W182–5.
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Author Information
  • Department of Genomic Sciences, School of Biological Sciences, Central University of Kerala, P.O. Central University, Kasaragod- 671314, India

  • Department of Genomic Sciences, School of Biological Sciences, Central University of Kerala, P.O. Central University, Kasaragod- 671314, India

  • Department of Genomic Sciences, School of Biological Sciences, Central University of Kerala, P.O. Central University, Kasaragod- 671314, India; Genomics & Molecular Medicine Unit, Institute of Genomics and Integrative Biology Council of Scientific and Industrial Research, Mathura Road, New Delhi-110025, INDIA

  • Centre for Genomics and Applied Gene Technology, Institute of Integrative Omics and Applied Biotechnology (IIOAB), Nonakuri, PurbaMedinipur, West Bengal-721172, INDIA

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    Ranjith Kumavath, Swaraj Prasad, Pratap Devarapalli, Debmalya Barh. (2014). In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20. International Journal of Genetics and Genomics, 2(4), 62-67. https://doi.org/10.11648/j.ijgg.20140204.13

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    Ranjith Kumavath; Swaraj Prasad; Pratap Devarapalli; Debmalya Barh. In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20. Int. J. Genet. Genomics 2014, 2(4), 62-67. doi: 10.11648/j.ijgg.20140204.13

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    AMA Style

    Ranjith Kumavath, Swaraj Prasad, Pratap Devarapalli, Debmalya Barh. In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20. Int J Genet Genomics. 2014;2(4):62-67. doi: 10.11648/j.ijgg.20140204.13

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  • @article{10.11648/j.ijgg.20140204.13,
      author = {Ranjith Kumavath and Swaraj Prasad and Pratap Devarapalli and Debmalya Barh},
      title = {In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20},
      journal = {International Journal of Genetics and Genomics},
      volume = {2},
      number = {4},
      pages = {62-67},
      doi = {10.11648/j.ijgg.20140204.13},
      url = {https://doi.org/10.11648/j.ijgg.20140204.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijgg.20140204.13},
      abstract = {Background: Globally, respiratory diseases cause an estimated 1.9 million deaths per year. One of the most important aetiological organisms of both adult and childhood respiratory disease is Non-Typeable Haemophilus influenzae (NTHi). NTHi is frequently isolated from the respiratory tract during episodes of sinusitis, Otitis Media and pneumonia and is the most common cause of Chronic Obstructive Pulmonary Disease (COPD) and bronchiectasis exacerbations. Methods: The work has been effectively complemented with the compilation of the Database of Essential Genes (DEG) of H. influenzae Rd KW20. Each protein is subjected to BLASTP in NCBI server http://www.ncbi.nlm.nih.gov/blastp. The candidate drug targets are determined by KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES. Results: The given gram negative bacteria H. influenzae Rd KW20 has six distinguished domains i.e., cytoplasmic, cytoplasmic membrane, periplasmic, outer membrane, extracellular and unknown domains. Out of 642 essential genes, the predicted non-human Homologous are 412 in the different domain of given bacteria. With the help of KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES, we successfully identified 35 novel drug targets which have common metabolic pathways both in host and pathogen & pathogen specific metabolic pathways. Conclusion: The novel drug targets suggest those genes which are active in both the host and pathogen metabolic pathway and should be a pathogen specific metabolic pathway. The important drug target regions are vacJ, lepB, emrB, MurG & dgkA. vacJ is present in outer membrane while lepB, emrB, MurG&dgkA are present in cytoplasmic membrane. All these genes are fully sequenced and specifically annotated in KEGG PATHWAY.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20
    AU  - Ranjith Kumavath
    AU  - Swaraj Prasad
    AU  - Pratap Devarapalli
    AU  - Debmalya Barh
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    N1  - https://doi.org/10.11648/j.ijgg.20140204.13
    DO  - 10.11648/j.ijgg.20140204.13
    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
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    EP  - 67
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20140204.13
    AB  - Background: Globally, respiratory diseases cause an estimated 1.9 million deaths per year. One of the most important aetiological organisms of both adult and childhood respiratory disease is Non-Typeable Haemophilus influenzae (NTHi). NTHi is frequently isolated from the respiratory tract during episodes of sinusitis, Otitis Media and pneumonia and is the most common cause of Chronic Obstructive Pulmonary Disease (COPD) and bronchiectasis exacerbations. Methods: The work has been effectively complemented with the compilation of the Database of Essential Genes (DEG) of H. influenzae Rd KW20. Each protein is subjected to BLASTP in NCBI server http://www.ncbi.nlm.nih.gov/blastp. The candidate drug targets are determined by KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES. Results: The given gram negative bacteria H. influenzae Rd KW20 has six distinguished domains i.e., cytoplasmic, cytoplasmic membrane, periplasmic, outer membrane, extracellular and unknown domains. Out of 642 essential genes, the predicted non-human Homologous are 412 in the different domain of given bacteria. With the help of KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES, we successfully identified 35 novel drug targets which have common metabolic pathways both in host and pathogen & pathogen specific metabolic pathways. Conclusion: The novel drug targets suggest those genes which are active in both the host and pathogen metabolic pathway and should be a pathogen specific metabolic pathway. The important drug target regions are vacJ, lepB, emrB, MurG & dgkA. vacJ is present in outer membrane while lepB, emrB, MurG&dgkA are present in cytoplasmic membrane. All these genes are fully sequenced and specifically annotated in KEGG PATHWAY.
    VL  - 2
    IS  - 4
    ER  - 

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