International Journal of Genetics and Genomics
Volume 4, Issue 1, February 2016, Pages: 1-4
Received: Mar. 3, 2016;
Accepted: Mar. 9, 2016;
Published: Mar. 22, 2016
Views 4726 Downloads 227
Alireza Goodarzi, "Armbiotechnology" Scientific and Production Center NAS RA, Yerevan, Armenia
Induced mutagenesis is widely used for selection of microorganisms producing biologically active substances and further improving of their activities. However, it is rarely used toward lactic acid bacteria (LAB) due to their genetic specificity. Determination of LABs sensitivity to UV light and evaluate the effectiveness of UV-induced mutagenesis by positive selection of antibiotic resistant mutants. Bacterial cells grown in LAPTg and NB broth up to late log phase were harvested by centrifugation and resuspended in phosphate buffer (pH 6.8) and irradiated by UV (15 w paired lamps) at distance of 30 cm and plated on LAPTg and NB agars with and without antibiotics for survivors count and positive selection of Rif and Str colonies. It was revealed that lactic acid bacteria were 1 to 2 log more resistant to UV in compression with E. coli. At dose 40 sec the difference in survival between L. lactis and E. coli was not so significant. The yield of rifampicin and streptomycin resistant mutants of LABs vary depending of UV exposure. The maximum yield about 2-3 log of both types of resistant mutants in lactobacilli and lactococcus were observed at dose 20 sec (0.1% survival). LAB possesses intrinsic resistance to UV irradiation 1-2 log higher than E. coli. The maximum yield of UV induced Rif and Str mutants in all LABs occurs at survival about 0.1%. Thus UV rays as an effective mutagen can be used in selection of dairy starters with improved technological and probiotics characteristic.
UV - Induced Mutagenesis in Lactic Acid Bacteria, International Journal of Genetics and Genomics.
Vol. 4, No. 1,
2016, pp. 1-4.
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Klaenhammer T. R (1995) Genetics of intestinal lactobacilli. Int Dairy J 5: 1019-1058.
Makarova K, Slesarev A, Wolf Y, et al (2006) Comparative genomics of the lactic acid bacteria. Proc Natl Acad Sci USA 103: 15611-15616.
Schroeter J. D, Klaenhammer T.R (2009) Genomics of lactic acid bacteria. FEMS Microbiol Lett 292: 1-6.
Lebeer S, Vanderleyden J, Keersmaecker S. De (2008) Genes and Molecules of Lactobacilli Supporting Probiotic Action, Microbiol. Mol. Biol. Rev 72 (4): 728-764.
Derkx P., Janzen T, Sørensen K. I, Christensen J. E, Lauridsen B.S, Johansen E (2014) The art of strain improvement of industrial lactic acid bacteria without the use of recombinant DNA technology. Microbial Cell Factories, 13 (Suppl 1): 5-13
Pfeiler E. A, Klaenhammer T. R (2007) The genomics of lactic acid bacteria. Trends in Microbiol 15: 546-553.
Holander S. K (1992) Genetics improvement of microbial starter culture. Application of Biotechnology to Traditional Fermented Foods. 20-26.
Hovhannisyan H. G, Barseghyan A. A, Grigoryan N. G, Topchyan A.V (2010) Genetic improvement of technological characteristics of startars for fermented milk products. Applied Biochemistry and Microbiology 46(4): 395-399.
Arihara K, Itoh M (2000) UV-induced Lactobacillus gasseri mutants resisting sodium chloride and sodium nitrite for meat fermentation. International Journal of Food Microbiology 56(2–3): 227-230.
Henriksen C. M, Nilsson D (2001) Redirection of pyruvate catabolism in Lactococcus lactis by selection of mutants with additional growth requirements. Appl Microbiol Biotechnol 56: 767-775.
Kuila R. K, Ranganathan B (1977) Ultraviolet light-induced mutants of Streptococcus lactis subspecies diacetylactis with enhanced acid- or flavor producing abilities. J Dairy Sci 61: 379-383.
Boumerdassi H, Monnet C, Desmazeaud M, Corrieu G (1997) Isolation and Properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 Mutants Producing Diacetyl and Acetoin from Glucose. Applied and Environmental Microbiology 63(6): 2293-2299.
Monnet C, Aymes F, Corrieu G (2000) Diacetyl and a-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in a-acetolactate decarboxylase and have a low lactate dehydrogenase activity. Appl Environ Microbiol 66: 5518-5520.
Rodríguez-Quiñones F, Palomares A.J, Megías M, Ruiz-Berraquero F (1984) The influence of several variables for nitrosoguanidine mutagenesis in Lactobacillus plantarum. Curr Microbiol 10: 137-140.
Miller J.H (1992) Mutagenesis with UV. A Short Course in Bacterial Genetics. Cold Spring Harbour Laboratory Press, New York, 150-156.
Chandrasekhar D, Houten B. V (2000) In vivo formation and repair of cyclobutane pyrimidine dimers and. 6-4 photoproducts measured at the gene and nucleotide level in Escherichia coli. Mutation Research. 450: 19-40.
Witkin E. M (1969) Ultraviolet-induced mutation and DNA repair. Annual Review of Genetics 23: 487-514.
Witkin E. M (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bateriological Reviews 40(4): 869-907.
Hastings J. W, Holzapfel W. H, Niemand J. G (1986) Radiation Resistance of Lactobacilli Isolated from Radurized Meat Relative to Growth and Environment. Applied and Environmental Microbiology 52(4): 898-90.
Gregory S. T, Cate J. H. D, and Dahlberg A. E (2001) Streptomycin-resistant and streptomycin-dependent mutants of the extreme thermophile Thermus thermophilus. J Mol Biol 309: 333-338.
Kogoma T (1994) Escherichia coli RNA polymerase mutants that enhance or diminish the SOS response constitutively expressed in the absence of RNase HI activity. J Bacteriol 176(5): 1521–1523.
Ding J. J, Carol A. Gross J, Gross D. J (1989) Characterization of the pleiotropic phenotypes of rifampin-resistant rpoB mutants of Escherichia coli. J. Bacteriol 171(9): 5229-5231.
Gorini L (1974) Streptomycin and misreading of the genetic code. In M. Nomura, A. Tissières, and P. Lengyel (ed.), Ribosomes. Cold Spring Harbor Laboratory, Cold Spring Harbor N.Y, 791-803.