Increasing evidence demonstrates that shortest more than mean telomere length predicts telomere dysfunction and genomic instability in association with a number of conditions, including cell senescence, aging and tumorigenesis. We developed Universal Single Cell Single Telomere Length Analysis (USC-STELA), based on a PCR-amplification and southern blotting, to measure short telomeres in individual cells. The mean short telomere length measured in individual cells by USC-STELA correlates with that from bulk cells, measured by Universal STELA (U-STELA). The validation and reproducibility of USC-STELA was confirmed using different cell types with known telomere lengths, as well as by using paired sister-cells from human embryos and cultured cells. Interestingly, individual cells known to elongate telomeres via alternative lengthening of telomeres (ALT) have more short telomeres, yet longer mean telomere length than control cells. Moreover, individual senescent fibroblasts carry more short telomeres compared to human embryonic stem cells (hESCs), consistent with the notion that short telomeres contribute to cellular senescence. Additionally, we found a greater load of short telomeres in polar bodies than in matching oocytes, providing further insights into the accelerated polar body DNA degradation following extrusion from the oocyte. USC-STELA provides a new method to study telomere dysfunction in individual cells, with potential to improve our understanding of the role of telomere dynamics in cancer, developmental biology and reproductive medicine.
| Published in | International Journal of Genetics and Genomics (Volume 7, Issue 3) |
| DOI | 10.11648/j.ijgg.20190703.14 |
| Page(s) | 60-68 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Single Cell, Telomere Length, Telomere Dysfunction, Cell Senescence, Genomic Instability
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APA Style
Fang Wang, Leroy Robinson, Yael Kramer, Keri Kalmbach, Paula Andrea Navarro, et al. (2019). Measurement of Short Telomere Load in Individual Cells. International Journal of Genetics and Genomics, 7(3), 60-68. https://doi.org/10.11648/j.ijgg.20190703.14
ACS Style
Fang Wang; Leroy Robinson; Yael Kramer; Keri Kalmbach; Paula Andrea Navarro, et al. Measurement of Short Telomere Load in Individual Cells. Int. J. Genet. Genomics 2019, 7(3), 60-68. doi: 10.11648/j.ijgg.20190703.14
AMA Style
Fang Wang, Leroy Robinson, Yael Kramer, Keri Kalmbach, Paula Andrea Navarro, et al. Measurement of Short Telomere Load in Individual Cells. Int J Genet Genomics. 2019;7(3):60-68. doi: 10.11648/j.ijgg.20190703.14
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Download@article{10.11648/j.ijgg.20190703.14,
author = {Fang Wang and Leroy Robinson and Yael Kramer and Keri Kalmbach and Paula Andrea Navarro and Ricardo Pimentel and Xinghua Victor Pan and Sherman Weissman and Lin Liu and David Keefe},
title = {Measurement of Short Telomere Load in Individual Cells},
journal = {International Journal of Genetics and Genomics},
volume = {7},
number = {3},
pages = {60-68},
doi = {10.11648/j.ijgg.20190703.14},
url = {https://doi.org/10.11648/j.ijgg.20190703.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20190703.14},
abstract = {Increasing evidence demonstrates that shortest more than mean telomere length predicts telomere dysfunction and genomic instability in association with a number of conditions, including cell senescence, aging and tumorigenesis. We developed Universal Single Cell Single Telomere Length Analysis (USC-STELA), based on a PCR-amplification and southern blotting, to measure short telomeres in individual cells. The mean short telomere length measured in individual cells by USC-STELA correlates with that from bulk cells, measured by Universal STELA (U-STELA). The validation and reproducibility of USC-STELA was confirmed using different cell types with known telomere lengths, as well as by using paired sister-cells from human embryos and cultured cells. Interestingly, individual cells known to elongate telomeres via alternative lengthening of telomeres (ALT) have more short telomeres, yet longer mean telomere length than control cells. Moreover, individual senescent fibroblasts carry more short telomeres compared to human embryonic stem cells (hESCs), consistent with the notion that short telomeres contribute to cellular senescence. Additionally, we found a greater load of short telomeres in polar bodies than in matching oocytes, providing further insights into the accelerated polar body DNA degradation following extrusion from the oocyte. USC-STELA provides a new method to study telomere dysfunction in individual cells, with potential to improve our understanding of the role of telomere dynamics in cancer, developmental biology and reproductive medicine.},
year = {2019}
}
TY - JOUR T1 - Measurement of Short Telomere Load in Individual Cells AU - Fang Wang AU - Leroy Robinson AU - Yael Kramer AU - Keri Kalmbach AU - Paula Andrea Navarro AU - Ricardo Pimentel AU - Xinghua Victor Pan AU - Sherman Weissman AU - Lin Liu AU - David Keefe Y1 - 2019/08/23 PY - 2019 N1 - https://doi.org/10.11648/j.ijgg.20190703.14 DO - 10.11648/j.ijgg.20190703.14 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 60 EP - 68 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20190703.14 AB - Increasing evidence demonstrates that shortest more than mean telomere length predicts telomere dysfunction and genomic instability in association with a number of conditions, including cell senescence, aging and tumorigenesis. We developed Universal Single Cell Single Telomere Length Analysis (USC-STELA), based on a PCR-amplification and southern blotting, to measure short telomeres in individual cells. The mean short telomere length measured in individual cells by USC-STELA correlates with that from bulk cells, measured by Universal STELA (U-STELA). The validation and reproducibility of USC-STELA was confirmed using different cell types with known telomere lengths, as well as by using paired sister-cells from human embryos and cultured cells. Interestingly, individual cells known to elongate telomeres via alternative lengthening of telomeres (ALT) have more short telomeres, yet longer mean telomere length than control cells. Moreover, individual senescent fibroblasts carry more short telomeres compared to human embryonic stem cells (hESCs), consistent with the notion that short telomeres contribute to cellular senescence. Additionally, we found a greater load of short telomeres in polar bodies than in matching oocytes, providing further insights into the accelerated polar body DNA degradation following extrusion from the oocyte. USC-STELA provides a new method to study telomere dysfunction in individual cells, with potential to improve our understanding of the role of telomere dynamics in cancer, developmental biology and reproductive medicine. VL - 7 IS - 3 ER -
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