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European Journal of Biophysics

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Comparative Study of the Reduction Process of Different Ring Structured Nitroxyl Spin Probes: An Electron Spin Resonance Study

V. Meenakumari, A. Jawahar, A. Milton Franklin Benial
Received: 23 March 2016    Accepted: 1 April 2016    Published: 26 April 2016
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Abstract

Electron spin resonance spectroscopy (ESR) studies on the reduction process of nitroxyl spin probes were carried out for 1mM concentration of 14N-labeled pyrrolidine nitroxyl spin probes, 3-carbamoyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (carbamoyl-PROXYL) and 3-carboxy-2,2,5,5-tetramethyl-pyrrolidine -1-oxyl (carboxy-PROXYL), 14N-labeled piperidine nitroxyl spin probes, 4-methoxy-2,2,6,6-tetramethyl-piperidine-1-oxyl (methoxy-TEMPO) and 4-acetamido-2,2,6,6-tetramethyl-piperidine-1-oxyl (acetamido-TEMPO) in 1 mM concentration of ascorbic acid as a function of time. The half-life time and decay rate were estimated. The piperidine nitroxyl spin probes show a short half-life time compared with that of pyrrolidine nitroxyl spin probes. This result indicates that the higher stability of pyrrolidine nitroxyl spin probes. The ESR was also recorded for 1mM concentration of pyrrolidine nitroxyl spin probes and piperidine nitroxyl spin probes in pure water using X-band ESR spectrometer. The ESR parameters such as line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were determined. These results indicate that the pyrrolidine nitroxyl spin probes have narrow line width and fast tumbling compared with the piperidine nitroxyl spin probes. Therefore, this study reveals that the pyrrolidine nitroxyl spin probes can act as good redox sensitive spin probes for free radical imaging.

DOI 10.11648/j.ejb.20160402.11
Published in European Journal of Biophysics (Volume 4, Issue 2, April 2016)
Page(s) 8-15
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

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Keywords

Electron Spin Resonances (ESR), Nitroxyl Spin Probe, Ascorbic Acid, Half Life Time, Decay Rate

References
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    V. Meenakumari, A. Jawahar, A. Milton Franklin Benial. (2016). Comparative Study of the Reduction Process of Different Ring Structured Nitroxyl Spin Probes: An Electron Spin Resonance Study. European Journal of Biophysics, 4(2), 8-15. https://doi.org/10.11648/j.ejb.20160402.11

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    V. Meenakumari; A. Jawahar; A. Milton Franklin Benial. Comparative Study of the Reduction Process of Different Ring Structured Nitroxyl Spin Probes: An Electron Spin Resonance Study. Eur. J. Biophys. 2016, 4(2), 8-15. doi: 10.11648/j.ejb.20160402.11

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    AMA Style

    V. Meenakumari, A. Jawahar, A. Milton Franklin Benial. Comparative Study of the Reduction Process of Different Ring Structured Nitroxyl Spin Probes: An Electron Spin Resonance Study. Eur J Biophys. 2016;4(2):8-15. doi: 10.11648/j.ejb.20160402.11

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  • @article{10.11648/j.ejb.20160402.11,
  author = {V. Meenakumari and A. Jawahar and A. Milton Franklin Benial},
  title = {Comparative Study of the Reduction Process of Different Ring Structured Nitroxyl Spin Probes: An Electron Spin Resonance Study},
  journal = {European Journal of Biophysics},
  volume = {4},
  number = {2},
  pages = {8-15},
  doi = {10.11648/j.ejb.20160402.11},
  url = {https://doi.org/10.11648/j.ejb.20160402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20160402.11},
  abstract = {Electron spin resonance spectroscopy (ESR) studies on the reduction process of nitroxyl spin probes were carried out for 1mM concentration of 14N-labeled pyrrolidine nitroxyl spin probes, 3-carbamoyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (carbamoyl-PROXYL) and 3-carboxy-2,2,5,5-tetramethyl-pyrrolidine -1-oxyl (carboxy-PROXYL), 14N-labeled piperidine nitroxyl spin probes, 4-methoxy-2,2,6,6-tetramethyl-piperidine-1-oxyl (methoxy-TEMPO) and 4-acetamido-2,2,6,6-tetramethyl-piperidine-1-oxyl (acetamido-TEMPO) in 1 mM concentration of ascorbic acid as a function of time. The half-life time and decay rate were estimated. The piperidine nitroxyl spin probes show a short half-life time compared with that of pyrrolidine nitroxyl spin probes. This result indicates that the higher stability of pyrrolidine nitroxyl spin probes. The ESR was also recorded for 1mM concentration of pyrrolidine nitroxyl spin probes and piperidine nitroxyl spin probes in pure water using X-band ESR spectrometer. The ESR parameters such as line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were determined. These results indicate that the pyrrolidine nitroxyl spin probes have narrow line width and fast tumbling compared with the piperidine nitroxyl spin probes. Therefore, this study reveals that the pyrrolidine nitroxyl spin probes can act as good redox sensitive spin probes for free radical imaging.},
 year = {2016}
}

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  • TY  - JOUR
T1  - Comparative Study of the Reduction Process of Different Ring Structured Nitroxyl Spin Probes: An Electron Spin Resonance Study
AU  - V. Meenakumari
AU  - A. Jawahar
AU  - A. Milton Franklin Benial
Y1  - 2016/04/26
PY  - 2016
N1  - https://doi.org/10.11648/j.ejb.20160402.11
DO  - 10.11648/j.ejb.20160402.11
T2  - European Journal of Biophysics
JF  - European Journal of Biophysics
JO  - European Journal of Biophysics
SP  - 8
EP  - 15
PB  - Science Publishing Group
SN  - 2329-1737
UR  - https://doi.org/10.11648/j.ejb.20160402.11
AB  - Electron spin resonance spectroscopy (ESR) studies on the reduction process of nitroxyl spin probes were carried out for 1mM concentration of 14N-labeled pyrrolidine nitroxyl spin probes, 3-carbamoyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (carbamoyl-PROXYL) and 3-carboxy-2,2,5,5-tetramethyl-pyrrolidine -1-oxyl (carboxy-PROXYL), 14N-labeled piperidine nitroxyl spin probes, 4-methoxy-2,2,6,6-tetramethyl-piperidine-1-oxyl (methoxy-TEMPO) and 4-acetamido-2,2,6,6-tetramethyl-piperidine-1-oxyl (acetamido-TEMPO) in 1 mM concentration of ascorbic acid as a function of time. The half-life time and decay rate were estimated. The piperidine nitroxyl spin probes show a short half-life time compared with that of pyrrolidine nitroxyl spin probes. This result indicates that the higher stability of pyrrolidine nitroxyl spin probes. The ESR was also recorded for 1mM concentration of pyrrolidine nitroxyl spin probes and piperidine nitroxyl spin probes in pure water using X-band ESR spectrometer. The ESR parameters such as line width, hyperfine coupling constant, g-factor, signal intensity ratio and rotational correlation time were determined. These results indicate that the pyrrolidine nitroxyl spin probes have narrow line width and fast tumbling compared with the piperidine nitroxyl spin probes. Therefore, this study reveals that the pyrrolidine nitroxyl spin probes can act as good redox sensitive spin probes for free radical imaging.
VL  - 4
IS  - 2
ER  -

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Author Information

  • V. Meenakumari

    Department of Physics, NMSSVN College, Nagamalai, Madurai, Tamilnadu, India

  • A. Jawahar

    Department of Chemistry, NMSSVN College, Nagamalai, Madurai, Tamilnadu, India

  • A. Milton Franklin Benial

    Department of Physics, NMSSVN College, Nagamalai, Madurai, Tamilnadu, India

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