Antioxidant Capacity of Essential Oils of Two Juniperus Species from Northwest of Algeria
European Journal of Biophysics
Volume 4, Issue 6, December 2016, Pages: 67-70
Received: Sep. 18, 2016; Accepted: Sep. 27, 2016; Published: Feb. 11, 2017
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Bachir Raho Ghalem, Department of Biology, University of Mascara, Mascara, Algeria
Otsmane Malika, Department of Biology, University of Mascara, Mascara, Algeria
Sebaa Fatiha, Department of Biology, University of Mascara, Mascara, Algeria
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This study was performed to evaluate the in vitro antioxidant activities of Juniperus phoenicea and Juniperus oxycedrus essential oils by three different methods, revealing an important antioxidant potential for both species. The results of the total antioxidant capacity of the EO's were 212.42 mg AE / g DM for J.phoenicea and 88.14 mg AE / g DM for J.oxycedrus. The effective concentration that reduces 50% of the DPPH solution is 1.279 g / ml for the J. phoenicea EO's and 6.56 µg/ml for J.oxycedrus EO's. With the method of FRAP, the two essential oils tested showed moderate reducing activity and significantly lower than ascorbic acid. The results indicated that Juniperus species have shown antioxidant activity which could be potential candidates for preparation of natural antioxidant drug or additive preparation.
Essential Oil, J. oxycedrus and J. phoenicea, Antioxidant Activities
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Bachir Raho Ghalem, Otsmane Malika, Sebaa Fatiha, Antioxidant Capacity of Essential Oils of Two Juniperus Species from Northwest of Algeria, European Journal of Biophysics. Vol. 4, No. 6, 2016, pp. 67-70. doi: 10.11648/j.ejb.20160406.12
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P. A. Cerutti, Oxidant stress and carcinogenesis, Eur J Clin Invest. 21 (1991) 1–11.
E. Shacter, Quantification and significance of protein oxidation in biological samples, Drug Metabolism Reviews. 32 (2000) 307–326.
I. Fridovich, Fundamental aspects of reactive oxygen species, or what's the matter with oxygen? Ann N Y Acad Sci. 893 (1999) 13-8.
Y. Z. Fang, S. Yang, G. Wu, Free radicals, antioxidants, and nutrition, Nutrition. 18 (2002) 872-9.
T. Kutlu, K. Takim, B. Çeken and M. Kizil, DNA damage protecting activity and in vitro antioxidant potential of the methanol extract of Cherry(Prunus avium L), J. Med. Plants Res. 8 (2014) 715-726.
Y. S. Çakmak, A. Aktumsek, A. Duran, Studies on antioxidant activity, volatile compound and fatty acid composition of different parts of Glycyrrhiza echinata L, EXCLI J. 11 (2012) 178–187.
S. Chanda, J. Parekh, Y. Baravalia, S. Parekh, Antimicrobial and antioxidant efficacy of various solvent extract of seeds and fruit rind of Caesalpinia pulcherrima Swartz, Archives of clinical Microbiology. 1 No. 4 (2010): 5 doi: 10:3823/218.
A. Farjon, World checklist and bibliography of conifers, Royal Botanic Gardens Kew. Chemistry Natural Compounds, 44 (1998) p. 2008.
R. P. Adams, T. Demeke, H. A. Abulfatih, RAPD DNA fingerprints and terpenoids: clues to past migrations of Juniperus in Arabia and east Africa, Theor. Appl. Genet. 87 (1993) 22-26.
T. N. Orhan, E. Akkol, F. Ergun, Evaluation of antiinflammatory and antinociceptive effects of some Juniperus species growing in Turkey, Turk J Biol. 36 (2012) 719-726.
E. Derwich, Z. Benziane, R. Taouil, O. Senhaji, M. Touzani, A comparative study of the chemical composition of the leaves volatile oil of Juniperus phoenicea and Juniperus oxycedrus, Middle -East journal of scientific Research. 5 (2010) 416-424.
P. Prieto, M. Pineda, M. Aguilar, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E, Anal. Biochem. 269 (1999) 337–341.
E. W. C. Chan, Y. Y. Lim, M. Omar, Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. J. Food Chem, 104 (2007) 1586-1593.
H. Zhao, W. Fan, J. Dong, J. Lu, J. Chen, L Shan, Y Lin, W. Kong. Evaluation of antioxidant activities and total phenolic contents of typical malting barley varieties, Food Chem. 107 (2008) 296–304.
H. Amiri, Antioxidant Activity of the Essential Oil and Methanolic Extract of Teucrium orientale (L.) subsp. taylori(Boiss.) Rech. f, Iran J Pharm Res. 9 (2010) 417–423.
N. Erkan, G. Ayranci, E. Ayranci, Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol, Food Chemistry. 110 (2008) 76–82.
I. F. Benzie, J. J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay, Anal. Biochem. 239(1996) 70–76.
F. Menaceur, A. Benchabane, M. H. A. Baaliouamer, Chemical Composition and Antioxidant Activity of Algerian Juniperus phoenicea L. Extracts, Journal of Biologically Active Products from Nature. 3 (2013) 87-96.
Satrani B, Ghanmi M, Mansouri N, Amusant N, Antioxidant properties of essential oils extracted from three species of Moroccan junipers, Environmental Science: An Indian Journal. 11 (2015) 239-247.
M. R. Loizzo, R. Tundis, F. Conforti, A. M. Saab, G. A. Statti, F. Menichini, Comparative chemical composition, Antioxidant and hypoglycaemic activities of Juniperus oxycedrus ssp, oxycedrus L. berry and wood oils from Lebanon, Food Chemistry Journal. 105 (2007) 572-578.
S. A. Emami, J. Asili, Z. Mohagheghi, M. K. Hassanzadeh, Antioxidant activity of leaves and fruits of Iranian conifers, eCAM. 11 (2007) 1-7.
N. Bouzouita, F. Kachouri, Ben M. Halima, M. M. Chaabouni, Composition chimique et activités antioxydante, antimicrobienne et insecticide de l'huile essentielle de Juniperus phoenicea, Journal de la Société Chimique de Tunisie. 10 (2008) 119-125.
M. F. Elmhdwi, I. H. Attitalla, B. A. Khan, Evaluation of Antibacterial Activity and Antioxidant Potential of Different Extracts from the Leaves of Juniperus Phoenicea, J Plant Pathol Microb. 6 (2015) 300.
M. El Jemli, R. Kamal, I. Marmouzi, A. Zerrouki, Y. Cherrah, K. Alaoui, Radical-Scavenging Activity and Ferric Reducing Ability of Juniperus thurifera (L.), J. oxycedrus (L.), J. phoenicea (L.) and Tetraclinis articulata (L.). Advances in Pharmacological Sciences. 2016; 2016: 6392656. doi:10.1155/2016/6392656.
S. Moein, M. Moein, Antioxidant Activities and Phenolic Content of Juniperus excelsa Extract. Iranian Journal of Pharmaceutical Sciences: 6 (2010) 133-140.
J. Yang, J. Guo, J. Yuan, In vitro antioxidant properties of rutin, LWT-Food Sci. Technol. 41 (2008) 1060–1066.
C. Bischin, F. Deac, R. Silaghi-Dumitrescu, J. A. Worrall, B. S. Rajagopal, G. Damian, C. E. Cooper, Ascorbate peroxidase activity of cytochrome c, Free Radic. Res. 45 (2011) 439–444.
T. E. Moussa-Ayoub, S. K. El-Samahy, L. W. Kroh, S. Rohn, Identification and quantification of flavonol aglycons in cactus pear (Opuntia ficus indica) fruit using a commercial pectinase and cellulose preparation, Food Chem. 124 (2011) 1177–1184.
H. Medini, A. Elaissi, M. L. Khouja, R. Chemli, Phytochemical screening and antioxidant activity of Juniperus phoenicea ssp. phoenicea L. extracts from two Tunisian locations, Journal of Experimental Biology and Agricultural Sciences. 1 (2013) 77-82.
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