Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract
European Journal of Biophysics
Volume 5, Issue 2, April 2017, Pages: 38-47
Received: Mar. 20, 2017; Accepted: Mar. 31, 2017; Published: May 8, 2017
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
William Dean Plikerd, Trivedi Global, Inc., Henderson, Nevada, USA
Peter L. Surguy, Trivedi Global, Inc., Henderson, Nevada, USA
Robert John Kock, Trivedi Global, Inc., Henderson, Nevada, USA
Rolando Baptista Piedad, Trivedi Global, Inc., Henderson, Nevada, USA
Russell Phillip Callas, Trivedi Global, Inc., Henderson, Nevada, USA
Sakina A. Ansari, Trivedi Global, Inc., Henderson, Nevada, USA
Sandra Lee Barrett, Trivedi Global, Inc., Henderson, Nevada, USA
Sara Friedman, Trivedi Global, Inc., Henderson, Nevada, USA
Steven Lee Christie, Trivedi Global, Inc., Henderson, Nevada, USA
Su-Mei Chen Liu, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Elizabeth Starling, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Jones, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Mardis Allen, Trivedi Global, Inc., Henderson, Nevada, USA
Susanne Kathrin Wasmus, Trivedi Global, Inc., Henderson, Nevada, USA
Terry Ann Benczik, Trivedi Global, Inc., Henderson, Nevada, USA
Thomas Charles Slade, Trivedi Global, Inc., Henderson, Nevada, USA
Thomas Orban, Trivedi Global, Inc., Henderson, Nevada, USA
Victoria L. Vannes, Trivedi Global, Inc., Henderson, Nevada, USA
Victoria Margot Schlosser, Trivedi Global, Inc., Henderson, Nevada, USA
Yusif Sarkis Yamin Albino, Trivedi Global, Inc., Henderson, Nevada, USA
Kalyan Kumar Sethi, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Parthasarathi Panda, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
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Abstract
Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.
Keywords
Withania somnifera, The Trivedi Effect®, Biofield Energy Healers, Energy of Consciousness Healing Treatment, Withanolides, LC-MS, GC-MS
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana, Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract, European Journal of Biophysics. Vol. 5, No. 2, 2017, pp. 38-47. doi: 10.11648/j.ejb.20170502.12
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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