The Genes such as carbonic anhydrase IX (CAIX), hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) have been suggested as hypoxic biomarkers in cancer. Indeed, these endogenous biomarkers have been shown to have stronger prognostic value response after treatment by irradiation. However, inconsistent results suggest that factors other than oxygen influence their expression. This present study deciphers the level of expression of different radioresistance biomarkers in both normoxia and hypoxia conditions followed by irradiation of human ovarian tumor cell lines (uterine cervix squamous cell carcinoma (HeLa). HeLa cells were submitted to hypoxia (1% O2) conditions in a Thermo Scientific Heracell i CO2 incubator. The cells were subjected to two doses 4-10 Gy irradiation and re-incubate in their starting conditions for 4 hours, then fixed in 4% paraformaldehyde for 20 min. Protein expressions were assessed by immunocytochemistry staining and fluorescent images were captured by a Axio Imager Z1 fluorescence microscope with oil immersion lens at 63× magnification. In normoxia conditions there was no modification of the level of expression of the CAIX after irradiation. However, an increasing expression level of VEGF was noted. The level of expression of HIF-1 in normoxia was low compared to the other two proteins (CAIX and VEGF). Hypoxia conditions at 2% resulted in a low expression of CAIX and VEGF before and after irradiation at 10 Gy in HeLa cells. HIF-1 had a maximum expression level compared to CAIX and VEGF at 2% oxygen after irradiation in HeLa cells. As tumor hypoxia occurs in a deprived microenvironment, other environmental factors such as irradiation might interact with the effect of low oxygen concentration on gene expression. This study shows that irradiation of HeLa cells has a profound influence on the oxygen dependent induction of certain endogenous hypoxic markers as HIF-1, CAIX, and VEGF.
| Published in | European Journal of Biophysics (Volume 8, Issue 2) |
| DOI | 10.11648/j.ejb.20200802.18 |
| Page(s) | 76-81 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Radioresistance, Biomarkers, Hypoxia, Normoxia, DNA Repair HELA Cells
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APA Style
Mamadou Soumboundou, Marie Thérèse Aloy, Macoura Gadji, Celine Malesys, Gora Mbaye, et al. (2020). Determination of Endogenous Radioresistance Biomarkers on HeLa Cells. European Journal of Biophysics, 8(2), 76-81. https://doi.org/10.11648/j.ejb.20200802.18
ACS Style
Mamadou Soumboundou; Marie Thérèse Aloy; Macoura Gadji; Celine Malesys; Gora Mbaye, et al. Determination of Endogenous Radioresistance Biomarkers on HeLa Cells. Eur. J. Biophys. 2020, 8(2), 76-81. doi: 10.11648/j.ejb.20200802.18
AMA Style
Mamadou Soumboundou, Marie Thérèse Aloy, Macoura Gadji, Celine Malesys, Gora Mbaye, et al. Determination of Endogenous Radioresistance Biomarkers on HeLa Cells. Eur J Biophys. 2020;8(2):76-81. doi: 10.11648/j.ejb.20200802.18
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Download@article{10.11648/j.ejb.20200802.18,
author = {Mamadou Soumboundou and Marie Thérèse Aloy and Macoura Gadji and Celine Malesys and Gora Mbaye and Ahmadou Dem and Mounibe Diarra and Claire Rodriguez-Lafrasse},
title = {Determination of Endogenous Radioresistance Biomarkers on HeLa Cells},
journal = {European Journal of Biophysics},
volume = {8},
number = {2},
pages = {76-81},
doi = {10.11648/j.ejb.20200802.18},
url = {https://doi.org/10.11648/j.ejb.20200802.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20200802.18},
abstract = {The Genes such as carbonic anhydrase IX (CAIX), hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) have been suggested as hypoxic biomarkers in cancer. Indeed, these endogenous biomarkers have been shown to have stronger prognostic value response after treatment by irradiation. However, inconsistent results suggest that factors other than oxygen influence their expression. This present study deciphers the level of expression of different radioresistance biomarkers in both normoxia and hypoxia conditions followed by irradiation of human ovarian tumor cell lines (uterine cervix squamous cell carcinoma (HeLa). HeLa cells were submitted to hypoxia (1% O2) conditions in a Thermo Scientific Heracell i CO2 incubator. The cells were subjected to two doses 4-10 Gy irradiation and re-incubate in their starting conditions for 4 hours, then fixed in 4% paraformaldehyde for 20 min. Protein expressions were assessed by immunocytochemistry staining and fluorescent images were captured by a Axio Imager Z1 fluorescence microscope with oil immersion lens at 63× magnification. In normoxia conditions there was no modification of the level of expression of the CAIX after irradiation. However, an increasing expression level of VEGF was noted. The level of expression of HIF-1 in normoxia was low compared to the other two proteins (CAIX and VEGF). Hypoxia conditions at 2% resulted in a low expression of CAIX and VEGF before and after irradiation at 10 Gy in HeLa cells. HIF-1 had a maximum expression level compared to CAIX and VEGF at 2% oxygen after irradiation in HeLa cells. As tumor hypoxia occurs in a deprived microenvironment, other environmental factors such as irradiation might interact with the effect of low oxygen concentration on gene expression. This study shows that irradiation of HeLa cells has a profound influence on the oxygen dependent induction of certain endogenous hypoxic markers as HIF-1, CAIX, and VEGF.},
year = {2020}
}
TY - JOUR T1 - Determination of Endogenous Radioresistance Biomarkers on HeLa Cells AU - Mamadou Soumboundou AU - Marie Thérèse Aloy AU - Macoura Gadji AU - Celine Malesys AU - Gora Mbaye AU - Ahmadou Dem AU - Mounibe Diarra AU - Claire Rodriguez-Lafrasse Y1 - 2020/12/28 PY - 2020 N1 - https://doi.org/10.11648/j.ejb.20200802.18 DO - 10.11648/j.ejb.20200802.18 T2 - European Journal of Biophysics JF - European Journal of Biophysics JO - European Journal of Biophysics SP - 76 EP - 81 PB - Science Publishing Group SN - 2329-1737 UR - https://doi.org/10.11648/j.ejb.20200802.18 AB - The Genes such as carbonic anhydrase IX (CAIX), hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) have been suggested as hypoxic biomarkers in cancer. Indeed, these endogenous biomarkers have been shown to have stronger prognostic value response after treatment by irradiation. However, inconsistent results suggest that factors other than oxygen influence their expression. This present study deciphers the level of expression of different radioresistance biomarkers in both normoxia and hypoxia conditions followed by irradiation of human ovarian tumor cell lines (uterine cervix squamous cell carcinoma (HeLa). HeLa cells were submitted to hypoxia (1% O2) conditions in a Thermo Scientific Heracell i CO2 incubator. The cells were subjected to two doses 4-10 Gy irradiation and re-incubate in their starting conditions for 4 hours, then fixed in 4% paraformaldehyde for 20 min. Protein expressions were assessed by immunocytochemistry staining and fluorescent images were captured by a Axio Imager Z1 fluorescence microscope with oil immersion lens at 63× magnification. In normoxia conditions there was no modification of the level of expression of the CAIX after irradiation. However, an increasing expression level of VEGF was noted. The level of expression of HIF-1 in normoxia was low compared to the other two proteins (CAIX and VEGF). Hypoxia conditions at 2% resulted in a low expression of CAIX and VEGF before and after irradiation at 10 Gy in HeLa cells. HIF-1 had a maximum expression level compared to CAIX and VEGF at 2% oxygen after irradiation in HeLa cells. As tumor hypoxia occurs in a deprived microenvironment, other environmental factors such as irradiation might interact with the effect of low oxygen concentration on gene expression. This study shows that irradiation of HeLa cells has a profound influence on the oxygen dependent induction of certain endogenous hypoxic markers as HIF-1, CAIX, and VEGF. VL - 8 IS - 2 ER -
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