European Journal of Biophysics

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Influence of Whole Body X-irradiation on the Liver and Possible Protective Effect by the Compatible Solute Ectoine in a Mouse Model

Received: 14 June 2016    Accepted: 27 June 2016    Published: 13 July 2016
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Abstract

Exposure of mammals to ionizing radiation leads to the development of a complex dose-dependent series of physiological and pathological changes. The present study was undertaken to investigate the effect of whole body x-irradiation of male mice with a single w dose of 6 Gy on the levels of some cytokines and parameters of oxidative stress, after one and seven days, in the liver. The possible protective effect of the compatible solute ectoine, which is known to reduce cell stress effects on the molecular level, was evaluated. The x-irradiated mice showed elevated interleukins in the liver. The levels of both the pro-inflammatory (IL-1β and IL-6) and the anti-inflammatory (IL-10) interleukins reached several times the control value with quantitative differences. The disturbance by low-dose x-irradiation also affected PGE2. Malondialdehyde, used as indication of oxidative stress, was elevated in the livers of x-irradiated mice. Although, there were no statistically significant differences in the results of total glutathione among the irradiated mice as compared to control values, there were differences among the values of the reduced and oxidized forms. The calculated redox potentials, showed some variations in the different groups, but were all within the values indicating that the cells are in the proliferative phase and far from the apoptotic phase. Treatment with ectoine modulated all the tested biochemical effects induced by x-irradiation in the liver. All the tested parameters had a tendency to go back to near control values.

DOI 10.11648/j.ejb.20160403.11
Published in European Journal of Biophysics (Volume 4, Issue 3, June 2016)
Page(s) 16-21
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ionizing Radiation, Cytokines, Ectoine, Interleukins, Malondialdehyde, Redox Potentials

References
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Author Information
  • Department of Pharmacology, Medical Research Institute, Alexandria University, Alexandria, Egypt

  • Department of Pharmacology, Medical Research Institute, Alexandria University, Alexandria, Egypt

  • Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt

  • Department of Medical Biophysics, Medical Research Institute, Alexandria University, Alexandria, Egypt

  • Department of Pharmacology, Medical Research Institute, Alexandria University, Alexandria, Egypt

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    Sarhan D. A., Shehata R. R., Kandil Y. I., Kotb M. A., El-Bassiouni E. A. (2016). Influence of Whole Body X-irradiation on the Liver and Possible Protective Effect by the Compatible Solute Ectoine in a Mouse Model. European Journal of Biophysics, 4(3), 16-21. https://doi.org/10.11648/j.ejb.20160403.11

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    ACS Style

    Sarhan D. A.; Shehata R. R.; Kandil Y. I.; Kotb M. A.; El-Bassiouni E. A. Influence of Whole Body X-irradiation on the Liver and Possible Protective Effect by the Compatible Solute Ectoine in a Mouse Model. Eur. J. Biophys. 2016, 4(3), 16-21. doi: 10.11648/j.ejb.20160403.11

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    AMA Style

    Sarhan D. A., Shehata R. R., Kandil Y. I., Kotb M. A., El-Bassiouni E. A. Influence of Whole Body X-irradiation on the Liver and Possible Protective Effect by the Compatible Solute Ectoine in a Mouse Model. Eur J Biophys. 2016;4(3):16-21. doi: 10.11648/j.ejb.20160403.11

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  • @article{10.11648/j.ejb.20160403.11,
      author = {Sarhan D. A. and Shehata R. R. and Kandil Y. I. and Kotb M. A. and El-Bassiouni E. A.},
      title = {Influence of Whole Body X-irradiation on the Liver and Possible Protective Effect by the Compatible Solute Ectoine in a Mouse Model},
      journal = {European Journal of Biophysics},
      volume = {4},
      number = {3},
      pages = {16-21},
      doi = {10.11648/j.ejb.20160403.11},
      url = {https://doi.org/10.11648/j.ejb.20160403.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ejb.20160403.11},
      abstract = {Exposure of mammals to ionizing radiation leads to the development of a complex dose-dependent series of physiological and pathological changes. The present study was undertaken to investigate the effect of whole body x-irradiation of male mice with a single w dose of 6 Gy on the levels of some cytokines and parameters of oxidative stress, after one and seven days, in the liver. The possible protective effect of the compatible solute ectoine, which is known to reduce cell stress effects on the molecular level, was evaluated. The x-irradiated mice showed elevated interleukins in the liver. The levels of both the pro-inflammatory (IL-1β and IL-6) and the anti-inflammatory (IL-10) interleukins reached several times the control value with quantitative differences. The disturbance by low-dose x-irradiation also affected PGE2. Malondialdehyde, used as indication of oxidative stress, was elevated in the livers of x-irradiated mice. Although, there were no statistically significant differences in the results of total glutathione among the irradiated mice as compared to control values, there were differences among the values of the reduced and oxidized forms. The calculated redox potentials, showed some variations in the different groups, but were all within the values indicating that the cells are in the proliferative phase and far from the apoptotic phase. Treatment with ectoine modulated all the tested biochemical effects induced by x-irradiation in the liver. All the tested parameters had a tendency to go back to near control values.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Influence of Whole Body X-irradiation on the Liver and Possible Protective Effect by the Compatible Solute Ectoine in a Mouse Model
    AU  - Sarhan D. A.
    AU  - Shehata R. R.
    AU  - Kandil Y. I.
    AU  - Kotb M. A.
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    JF  - European Journal of Biophysics
    JO  - European Journal of Biophysics
    SP  - 16
    EP  - 21
    PB  - Science Publishing Group
    SN  - 2329-1737
    UR  - https://doi.org/10.11648/j.ejb.20160403.11
    AB  - Exposure of mammals to ionizing radiation leads to the development of a complex dose-dependent series of physiological and pathological changes. The present study was undertaken to investigate the effect of whole body x-irradiation of male mice with a single w dose of 6 Gy on the levels of some cytokines and parameters of oxidative stress, after one and seven days, in the liver. The possible protective effect of the compatible solute ectoine, which is known to reduce cell stress effects on the molecular level, was evaluated. The x-irradiated mice showed elevated interleukins in the liver. The levels of both the pro-inflammatory (IL-1β and IL-6) and the anti-inflammatory (IL-10) interleukins reached several times the control value with quantitative differences. The disturbance by low-dose x-irradiation also affected PGE2. Malondialdehyde, used as indication of oxidative stress, was elevated in the livers of x-irradiated mice. Although, there were no statistically significant differences in the results of total glutathione among the irradiated mice as compared to control values, there were differences among the values of the reduced and oxidized forms. The calculated redox potentials, showed some variations in the different groups, but were all within the values indicating that the cells are in the proliferative phase and far from the apoptotic phase. Treatment with ectoine modulated all the tested biochemical effects induced by x-irradiation in the liver. All the tested parameters had a tendency to go back to near control values.
    VL  - 4
    IS  - 3
    ER  - 

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