Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam
American Journal of Life Sciences
Volume 1, Issue 3, June 2013, Pages: 88-92
Received: May 2, 2013;
Published: Jun. 10, 2013
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Cao Ngoc Diep, Dept. Microbiology Biotechnology, Depertment of Rural Development, Can Tho City, Vietnam;Agriculture and Rural Development Dept., Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam
Than Ngoc Hieu, Dept. Microbiology Biotechnology, Depertment of Rural Development, Can Tho City, Vietnam;Agriculture and Rural Development Dept., Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam
Twenty-five strains were isolated on Aleksandrov medium from sample soils/weathered rocks of Ha Tien Mountain, Kien Giang, Vietnam. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Seventeen of twenty-five bacterial strains were identified by PCR technique with specific primers fd1 and rP, they were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>10 mg l-1 P2O5 and >50 mg l-1 K2O) effectively. These seven strains were chosen to sequence, DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The results showed that DNV16 strain was similarity of 99% with EU977655.1 (Microbacterium hominis), TC1D strain was a 99% similarity with FN547417.1 (Flectobacillus sp.), CH9E strain was a 99% similarity of 99% with GQ861463.1 (Agrobacterium tumefasciens), TC1A strain was similarity of 99% with HM003210.1 (Bacillus cereus) and FJ976533.1 (Bacillus coagulans), CH7A strain was 99% similarity with EU048539.1 (Bacillus cereus) and GQ214131.1 (Bacillus subtilis) and TD6B strain was a 99% similarity wih AB301013.1 (Bacillus subtilis) and CH7D strain was similarity of 98% with FJ976616.1 (Bacillus megaterium). Seven strains related with Bacillus megaterium and Bacillus coagulans closely in phylogenetic tree.
Cao Ngoc Diep,
Than Ngoc Hieu,
Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam, American Journal of Life Sciences.
Vol. 1, No. 3,
2013, pp. 88-92.
A.H. Goldstein, Involvement of the quinoprotein glucose dehydrogenase in the solubilization of exogenous phosphate by gram-negative bacteria. In: Torriani-Gorini, A., E. Yagil, S. Silver. Phosphate in Microorganisms: Cellular and Molecular Biology. Washington, DC. ASM Press, 1994, pp.197 - 203.
C.S.Cockell, D. Pybus, K. Olsson-Francis, L. Kelly, D. Petley, N. Rosser, K. Howard, and F. Mosselmans, Molecular Characterization and Geological Microenvironment of a Microbial Community Inhabiting Weathering Receding Shale Cliffs, Microb. Ecol. vol (61), 2011, pp. 166-181.
H. Xiufang, C. Jishuang and G. Jiangfeng, Two phosphate- and potassium- solubilizing bacteria isolated from Tianmu Moutain, Zhejiang, China. World Journal of Microbiology and Biotechnology. vol (22), 2006, pp. 983 - 990.
N.V. Thanh, V.T.Xuan, L.Q.Tri and V.Q.Minh, Studies on main crop systems cultivated on sulfaquept soils in Mekong Delta. MSc. Thesis, Can Tho University, Vietnam. 1997
J.I. Sperberg, The incidence of apatite- solubilizing organisms in the rhizosphere and soil. Australian Journal of Agricultural and Resource Economics vol (9), 1958, p.778.
A.H. Goldstein, Bacterial solubilization of mineral phosphates: historical perspective and furture prospects. American Journal of Alternative Agriculture1, 1986, pp.51- 57.
V.G. Aleksandrov, R.N. Blagodyr and I.P. Ilev, Liberation of phosphoric aicd from apatite by silicate bacteria. Mikrobiolohichnyi Zhurnal (Kiev) vol(29), 1967, pp.111 - 114.
M. Manib, M.K. Zahra, A.L. Abdel, and A. Heggo, Role of solicate bacteria in releasing K and Si from biotite and orthoclase. In. Soil biology and Conservation of the Biosphere, ed. Szegi J. 1986, pp.733-743. Budapest, Akademiai Kiado, ISBN 9630537001.
B. Neumann, A. Pospiech and H.U. Schairrer, Rapid isolation of genomic DNA from Gram – negative bacteria. Trends Genet, vol (8), 1992, pp.332 - 333.
K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar, "MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods," Mol. Biol., vol. 28(10), 2011, pp. 2731–2739.
M. Datta, S. Banish, and R.K. Dupta, Studies on the efficiency of a phytohormone producing phosphate solubilizing Bacillus firmus in augmenting paddy yield in acid soils of Nagaland. Plant and Soil vol (69), 1982, pp.365-373.
C. N. Diep, T. T. Phong and T. T. Giang, Isolation and Selection of phosphate-solubilizing and IAA-biosynthesis Pseudomonas sp. Science and technology J. Agriculture and Rural Development. Ministry of Agriculture and Rural Development, Vietnam vol (9), 2009, pp.32-35.
Z.A. Burgstaller, H. Sreasser, H. Wobking and F. Shinner, Solubilization of zinc oxide from filterdust with Penicillium simplicassimum bioreactor, leaching and stoichiometry. Environmental Science and Technology vol (26), 1992, pp.340-346.
G.G. Nianikova, E.E. Kuprina, O.V. Pestova, and S.V. Vodolazhskaia, Immobilizing of Bacillus mucilaginosus, a producer of exopolysaccharides, on chitin. Priklalmaia Biokhilia I Mikrobiologiya vol (38), 2002, pp. 300-304.
E. Bird, Coastal geomorphology: an introduction, John Wiley and Sons, Incs, New York, 2000.
S.A. Welch, W.W. Barker, and J.F. Banfield, Microbial extracellular polysaccharides and plagioclase dissolution. Geochim Cosmochim. Acta. vol (63), 1999, pp.1405-1419.
P.C. Bennett, J.R. Rogers, W.J. Choi, and F.K. Hiebert, Silicates, silicate weathering, and microbial ecology. Geomicrobiol. J. vol (18), 2001, pp.3-19.
K.R. Glowa, J.M. Arocena, and H.B. Massicotte, Extraction of potassium and/or magnesium from selected soil minerals by Piloderma. Geomicrobiol. J., vol (20), 2003, pp. 99-111.
C.N. Diep, N.T. Don, T.T.H. Nhung, and L.T.D. Thanh, Isolation and identification of phosphate- and potassium-solubilizing bacteria in weathered materilas from calcacerous mountain, Kien Giang, Vietnam. Proceeding of the 2012 International Symposium on Biology Science and Environmental Engineering (ISBSEE 2012) organized at Chinam, Malaysia from 28-30th, 2012.