Curcumin Inhibits Compound 48/80 Induced Systemic Anaphylaxis
American Journal of Life Sciences
Volume 1, Issue 4, August 2013, Pages: 165-170
Received: Jun. 19, 2013; Published: Jul. 30, 2013
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Subhashini , Department of Zoology, MMV, Banaras Hindu University, Varanasi, India
Preeti Singh Chauhan, Department of Zoology, MMV, Banaras Hindu University, Varanasi, India
Sharda Kumari, Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India
Debabrata Dash, Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India
Rashmi Singh, Department of Zoology, MMV, Banaras Hindu University, Varanasi, India
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Curcumin, the active component of turmeric, is a polyphenolic phytochemical with anti-tumor, anti-inflammatory, anti-oxidative and anti-allergic properties. Mast cells participate in allergic inflammation by virtue of their ability of being activated to allergens and lead to the release of number of biologically active mediators including histamine, prostaglandins, leukotrienes, various cytokines etc. In this report, we have investigated effects of curcumin on non-immunological stimulations like Compound 48/80 induced systemic anaphylaxis. In vitro experiments have confirmed non-toxicity of curcumin (50µM) as assessed by MTT test but 100µM dose was found toxic. Curcumin (50µM) inhibited Compound 48/80 induced mouse peritoneal mast cell (MPMC) degranulation and histamine release in dose-dependent manner. Therefore, it is worth to study effect of curcumin on non-immunological stimulations as most often it occurs without IgE involvement. Whether it has mast cell membrane stabilizing activity or some other signaling mechanisms are involved underlying its potential could be explored further.
Compound 48/80, Curcumin, Histamine, Mast Cells, Systemic Anaphylaxis
To cite this article
Subhashini , Preeti Singh Chauhan, Sharda Kumari, Debabrata Dash, Rashmi Singh, Curcumin Inhibits Compound 48/80 Induced Systemic Anaphylaxis, American Journal of Life Sciences. Vol. 1, No. 4, 2013, pp. 165-170. doi: 10.11648/j.ajls.20130104.14
Sur R, Cavender D, Malviya R; Different approaches to study mast cell functions. Int immunopharmacol 7, 2007:555-67.
Hart PH Regulation of inflammatory response in asthma by mast cell products; Immunol Cell Biol 79, 2001: 49.
Kalesnikoff J, Galli SJ; New developments in mast cell biology; Nat Immunol 9, 2008,1215.
Galli SJ, Tsai M, Piliponsky AM; The development of allergic inflammation; Nature, 454, 2008, 445–454.
Ennis M, Pearce FL, Weston PM; Some studies on the release of histamine from mast cells stimulated with polylysine. Br J Pharmacol , 70,1980, 329.
Allansmith MR, Baird RS, Ross RN,Barney NP, Bloch KJ; Ocular anaphylaxis induced in rat by topical application of compound 48/80. Dose response and time course study, Acta Opthamol Suppl 192,1989, 145.
Marone G., Triggiani M, Genovese A, Paulis De; Role of human mast cells and basophils in bronchial asthma. Adv Immunol 88, 2005, 97.
Murray JJ, Tonnel AB, Brash AR, J. III, Roberts P, Gosset R Workman A. Capron and J.A. Oates; Release of Prostaglandin D2 into human airways during acute antigen challenge. N Engl J Med, 315,1986, 800.
Proud D, Sweet J, Stein P, Settipane RA, Kagey-Sobotka A, Friedlaender M.H. and Lichtenstein L.M; Inflammatory mediator release on conjunctival provocation of allergic subjects with allergen, J allergy Clin Immunol 85, 1990, 896.
Cui SX, Qu XJ, Xie YY, et al. Curcumin inhibits telomerase activity in human cancer cell lines. Int J Mol Med, 18,2006, 227–231.
Eybl V, Kotyzova D, Koutensky J; Comparative study of natural antioxidants - curcumin, resveratrol and melatonin - in cadmium-induced oxidative damage in mice. Toxicology 225,2006:150–156.
Kurup VP, Barrios CS; Immunomodulatory effects of curcumin in allergy. Mol Nutr Food Res, 52, 2008, 1031–1039.
Suzuki M, Nakamura T, Iyoki S, et al; Elucidation of anti-allergic activities of curcumin-related compounds with a special reference to their anti-oxidative activities. Biol Pharm Bull, 28, 2005,1438–1443.
Lee JH, Kim JW, Ko NY, et al; Curcumin, a constituent of curry, suppresses IgE-mediated allergic response and mast cell activation at the level of Syk. J Allergy Clin Immunol 121,2008,1225–1231.
Choi YH, Yan GH, Chai OH, et al; Inhibition of anaphylaxis like reaction and mast cell activation by water extract from the fruiting body of Phellinus linteus. Biol Phar Bull 29, 2006a, 1360.
Choi YH, Yan GH, Chai OH et al; Inhibitory effect of Agaricus blezei on mast cell mediated–anaphylaxis-like reactions. Biol Pharm Bull 29, 2006b, 1366.
Rashmi Singh, A.Nath, P.P.Gupta, M.Shukla, Sanjay K.Khare and B.Kundu. Antiallergic /antiasthmatic activity of oligopeptides related to IgE, Pharmacol. Res. 37, 1998, 353-356
Shore P., Purkhalter A and Cohn. Jr., V; A method for the fluorometric assay of histamine in tissues, J Pharmacol Exp Ther 127,1959: 182.
Yoshimura T, Hamaguchi E, Usami E, et al; Increased in vitro release of interferon-gamma from ampicillin-stimulated peripheral blood mononuclear cells in Stevens-Johnson syndrome. Biol Pharm Bull, 27, 2004, 929–931.
Sullivan TJ, Parker KL, Eisen SA, Parker CW Modulation of cyclic AMP in purified rat mast cells, II: studies on the relationship between intracellular cyclic AMP concentrations and histamine release. J Immunol, 114,1975,1480–1485.
Tasaka K, Mio M, Okamoto M. Intracellular calcium release induced by histamine releasers and its inhibition by some antiallergic drugs. Ann Allergy. 56,1986,464–469.
Allansmith MR, Baird RS, Ross RN, Barney NP, Bloch KJ. Ocular anaphylaxis induced in the rat by topical application of compound 48/80; Dose response and time course study, Acta Ophthalmol Suppl, 192,1989,145–153.
Galli SJ. New concepts about the mast cell; N Engl J Med, 328,1993, 257–265.
Palomäki VA, Laitinen JT; The basic secretagogue compound 48/80 activates G proteins indirectly via stimulation of phospholipase D-lysophosphatidic acid receptor axis and 5-HT1A receptors in rat brain sections; Br J Pharmacol 147, 2006,596–606.
Turner H, Kinet JP; Signalling through the high-affinity IgE receptor Fc epsilon RI. Nature 402s (6760 Suppl),1999,B24–B30.
Choi YH,Yan GH,Chang CH; Inhibitory effects of curcumin on passive cutaneous anaphylactoid response and compound 48/80-induced mast cell activation. Anat Cell Biol,Mar;43(1) 2010,36-43.
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