A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.

Amita Jain; Rashmi Ranade; Prem Pritam; Neelu Joshi; Sirisha Lakshmi Vavilala; Ankita Jain

doi:doi:10.11648/j.ajls.20140205.17

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A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.

Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, Ankita Jain

Published in American Journal of Life Sciences (Volume 2, Issue 5)

Received: 16 September 2014 Accepted: 20 October 2014 Published: 30 October 2014

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Abstract

Helicteres isora L., commonly known as Indian Screw Tree is a highly valued medicinal plant in South-East Asia. The various phytochemicals like phenols, flavonoids and other antioxidants that impart the medicinal properties in this plant, vary in their composition and concentration in different plant parts. In the present research, the total phenolic content, total flavonoids content and free radical scavenging activity (FRAP and DPPH assay) in fresh and dry sample extracts of leaf, bark, fruit and root of H. isora L., prepared in four different solvents (distilled water, ethanol, methanol and acetone) were studied, and their results compared using Pearson’s Correlation. The plant extracts were also subjected to RP-HPLC for detection and quantitation of naturally occurring phenolic compounds using six phenolic standards (Gallic acid, Vanillin, Catechol, Ferrulic acid, p-coumaric acid and Caffeic acid). The highest total phenolic content (7.22 mg/g GAE) and FRAP value (64.98 mg/g TE) were observed in aqueous dry root extract. The acetone extract of fresh leaf (57.08 mg/g of RE) was found richest in total flavonoids, while the methanolic extract of fresh fruit uniquely exhibited strong free radical scavenging activity as evidenced by the low IC50 value (34.37 mg/ml) in DPPH assay. The RP-HPLC analysis revealed that Catechol and Gallic acid were most abundantly found phenolic compounds in extracts of H. isora L. The total phenolic content showed strong positive correlation with free radical scavenging activity (FRAP and DPPH assays) in both fresh and dry plant parts, suggesting that phenols are the main compounds responsible for the antioxidant activity. The root of H. isora L. was found rich in phenolics and antioxidant capacity indicating its strong potential for medicinal use, followed by fruit, leaf and bark.

Published in	American Journal of Life Sciences (Volume 2, Issue 5)
DOI	10.11648/j.ajls.20140205.17
Page(s)	292-302
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Helicteres Isora L., Phenols, Flavonoids, DPPH, FRAP, RP-HPLC

References

[1]	Raja, C. Elanchezhiyan and S. Sethupathy, “Antihyperlipidemic activity of Helicteres isora fruit extract on streptozotocin induced diabetic male wistar rats,” European Review for Medical and Pharmacological Sciences, vol. 14, pp. 191-196, 2010.
[2]	L. Goldfrank, N. Lewin, N. Flomenbaum and M. Howland, “The Pernicious Panacea: Herbal Medicine,” Hospital physicians, vol. 10, pp. 64–86, 1982.
[3]	Vulto and P. Smet, “Drugs used in non-orthodox medicine,” In Meyler's side effects of drugs, vol.11, pp. 999-1005, 1988.
[4]	L. Mentz and E. Schenkel, “A coereˆ ncia e a confiabilidade das indicac¸o˜esterapeˆuticas,” Caderno de Farma´ cia, vol. 51(2), pp. 93-119, 1989.
[5]	F. Ripa, M. Haque and I. Bulbul, “In vitro antibacterial, cytotoxic and antioxidant activities of plant Nephelium longan,” Pakistan Journal of Biological Sciences, vol. 13(1), pp. 22-27, 2010.
[6]	N. Kumar and A. Singh, “Plant profile, phytochemistry and pharmacology of Avartani (Helicteres isora Linn.): A review,” Asian Pacific Journal of Tropical Biomedicine, vol. 4(1), pp. S22-S26, 2014.
[7]	G Kumar, G. Banu and A. Murugesan, “Effect of Helicteres isora bark extracts on heart antioxidant status and lipid peroxidation in streptozotocin diabetic rats,” Journal of applied biomedicine, vol. 6, pp. 89–95, 2008.
[8]	N. Pohocha and N. Grampurohit, “Antispasmodic activity of the fruits of Helicteres isora Linn,” Phytotherapy Research, vol. 15, pp. 49–52, 2001.
[9]	K. Singh, S. Saha and J. Maheswari, “Ethnobotany of Helicteres isora Linn. In Kheri district, Uttar Pradesh,” Journal of Economic & Taxonomic Botany, vol. 7(2), pp. 487-492, 1985.
[10]	G. Kumar, G. Banu, A. Murugesan, and M. Pandian, “Effect of Helicteres isora bark extracts on brain antioxidant status and lipid peroxidation in streptozotocin diabetic rats, Pharmaceutical Biology, vol. 45(10), pp. 753-759, 2007.
[11]	T. Kumar, A. Christy, R. Ramya, M. Malaisamy, C. Sivaraj and P. Arjun, “Antioxidant and anticancer activity of Helicteres isora dried fruit solvent extracts,” Journal of Academia and Industrial Research, vol. 1(3), pp. 148-152, 2012.
[12]	E. Varghese, S. Narayanan, R. Gopal, A. Nair, A. Chittethu and T. Anson, “Anticancer activity of chloroform extract of Helicteres isora,” International Journal of Pharmacy and Technology, vol. 3(2), pp. 2560-2564, 2011.
[13]	S. Venkatesh, K. Laxmi, B. Reddy and M. Ramesh, “Antinociceptive activity of Helicteres isora.” Fitoterapia, vol. 78(2), pp. 146-148, 2007.
[14]	M. Chitra and S. Prema, “Hepatoprotective activity of Helicteres isora Linn. against CCl4 induced hepatic damage in rats,” Hamdard Medicus, vol. 52(1), pp. 112-115, 2009.
[15]	P. Gayathri, D. Gayathri, S. Srinivasan and S. Saroja, “Screening and quantitation of phytochemicals and nutritional components of the fruit and bark of Helicteres isora,” Hygeia Journal for Drugs and Medicines, vol. 2(1), pp. 57-62, 2010.
[16]	N. Loganayaki, P. Siddhuraju and S. Manian, “Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L,” Journal of Food Science and Technology, DOI.10.1007/S 1397-011-0389-x, 2013.
[17]	T. Satake, K. Kamiya, Y. Saiki, T. Hama, Y. Fujimoto and S. Kitanaka, “Studies on the constituents of fruits of Helicteres isora L.,” Chemical and Pharmaceutical Bulletin, vol. 47(10), pp. 1444-1447, 1999.
[18]	P. Ramesh and C. Yuvarajan, “A new flavones methyl ether from H. isora,” Journal of Natural Products, vol. 58(8), pp. 1242-1243, 1995.
[19]	A. Jain, P. Sinha and N. Desai, “Estimation of flavonoid, phenol content and antioxidant potential of Indian screw tree (Helicteres isora L.),” International Journal of Pharmaceutical Sciences and Drug Research, vol. 5(4), pp. 1320-30, 2014.
[20]	S. Ugochukwu, A. Uche and O. Ifeanyi, “Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker,” Asian Journal of Plant Science and Research, vol. 3(3), pp. 10-13, 2013.
[21]	V. Pai, S. Sawant, A. Ghatak, P. Chaturvedi, A. Gupte and N. Desai, “Characterization of Indian beers: chemical composition and antioxidant potential,” Journal of Food Science and Technology, DOI.10.1007/S 13197-013-1152-2, 2013
[22]	S. Chanda and R. Dave, “In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview,” African Journal of Microbiology Research, vol. 3(13), pp. 981-996, 2009.
[23]	J. Domínguez-Avila, E. Alvarez-Parrilla, G. González-Aguilar, J.Villa-Rodríguez, G. Olivas-Orozco, J. Molina-Corral, M. Gómez-García and L. Rosa, “Influence of Growing Location on the Phytochemical Content of Pecan (Carya illinoinensis) Oil,” Journal of Food Research, vol. 2(5), pp. 143-151, 2013.
[24]	P. Tiwari, B. Kumar, M. Kaur, G. Kaur and H. Kaur, “Phytochemical screening and extraction: A review,” International Pharmaceutical sciencia, vol. 1, pp. 98-106, 2011.
[25]	M. Bhoyar, G. Mishra, P. Naik, and R. Srivastava, “Estimation of antioxidant activity and total phenolics among natural populations of Caper (Capparis spinosa) leaves collected from cold arid desert of trans-Himalayas,” Australian Journal of Crop Science, vol. 5(2), pp. 912-919, 2011.
[26]	M. Hossain, C. Barry-Ryan, A. Martin-Diana and N. Bunton. “Effect of drying method on the antioxidant capacity of six Larniaceae herbs,” Food Chemistry, vol. 123(1), pp. 85-91, 2010.
[27]	Bilal, C. Elanchezhiyan and S. Sethupathy, “In Vitro antioxidative role of Helicteres isora,” International journal of biomass & renewable, vol. 01(12), pp.177-183, 2012.
[28]	P. Sabale, N. Grampurohit, S. Banerjee, D. Gaikwad and M. Gadhave, “Recent advances on the phytochemical and pharmacological profile of plant Helicteres isora Linn,” International Research Journal of Pharmacy, vol. 3(4), pp. 14-16, 2012.
[29]	S. Vennila, S. Mohana, G. Bupesh, K. Mathiyazhagan, D. Dhanagaran, M. Baskar, S. Amutha and B. Leeba, “Qualitative Phytochemical screening and in vitro antioxidant activity of Helicteres isora L.” Herbal Tech Industry, pp. 14-18, 2012.
[30]	B. Polani, P. Krishnamoorthy, N. Deepthi and M. Nissi, “Evaluation of antioxidants and molecular docking studies of Helicteres isora fruit extracts,” Journal of Drug Delivery & Therapeutics, vol. 3(1), pp. 33-35, 2013.
[31]	Gawron-Gzella, M. Dudek-Makuch and I. Matlawska, “DPPH radical scavenging activity and phenolic compound content in different leaf extracts from selected blackberry species,” Acta Biologica Cracoviensia series Botanica, vol. 54(2), pp. 32–38, 2012.
[32]	M. Suthar, G. Rathod and M. Pareek, “Antioxidant and antidiabetic activity of Helicteres isora L. fruits,” Indian Journal of Pharmaceutical Sciences, vol. 71(6), pp. 695-699, 2009.
[33]	C. Henriquez, S. Almonacid, I. Cheiffelle, T. Valenzuela, M. Araya, L. Cabezas, R. Simpson and H. Speisky, “Determination of antioxidant capacity, total phenolic content and mineral composition of different fruit tissue of five apple cultivars grown in chile,” Chilean Journal of Agricultural Research, vol. 70(4), pp. 523-536, 2010
[34]	K. Thaipong, U. Boonprakob, K. Crosby, L. Cisneros-Zevallos and D. Byrne, “Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts,” Journal of Food Composition and Analysis, vol. 19, pp. 669–675, 2006.

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Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, et al. (2014). A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. American Journal of Life Sciences, 2(5), 292-302. https://doi.org/10.11648/j.ajls.20140205.17

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Amita Jain; Rashmi Ranade; Prem Pritam; Neelu Joshi; Sirisha Lakshmi Vavilala, et al. A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. Am. J. Life Sci. 2014, 2(5), 292-302. doi: 10.11648/j.ajls.20140205.17

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AMA Style

Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, et al. A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. Am J Life Sci. 2014;2(5):292-302. doi: 10.11648/j.ajls.20140205.17

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@article{10.11648/j.ajls.20140205.17,
  author = {Amita Jain and Rashmi Ranade and Prem Pritam and Neelu Joshi and Sirisha Lakshmi Vavilala and Ankita Jain},
  title = {A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {5},
  pages = {292-302},
  doi = {10.11648/j.ajls.20140205.17},
  url = {https://doi.org/10.11648/j.ajls.20140205.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140205.17},
  abstract = {Helicteres isora L., commonly known as Indian Screw Tree is a highly valued medicinal plant in South-East Asia. The various phytochemicals like phenols, flavonoids and other antioxidants that impart the medicinal properties in this plant, vary in their composition and concentration in different plant parts. In the present research, the total phenolic content, total flavonoids content and free radical scavenging activity (FRAP and DPPH assay) in fresh and dry sample extracts of leaf, bark, fruit and root of H. isora L., prepared in four different solvents (distilled water, ethanol, methanol and acetone) were studied, and their results compared using Pearson’s Correlation. The plant extracts were also subjected to RP-HPLC for detection and quantitation of naturally occurring phenolic compounds using six phenolic standards (Gallic acid, Vanillin, Catechol, Ferrulic acid, p-coumaric acid and Caffeic acid). The highest total phenolic content (7.22 mg/g GAE) and FRAP value (64.98 mg/g TE) were observed in aqueous dry root extract. The acetone extract of fresh leaf (57.08 mg/g of RE) was found richest in total flavonoids, while the methanolic extract of fresh fruit uniquely exhibited strong free radical scavenging activity as evidenced by the low IC50 value (34.37 mg/ml) in DPPH assay. The RP-HPLC analysis revealed that Catechol and Gallic acid were most abundantly found phenolic compounds in extracts of H. isora L. The total phenolic content showed strong positive correlation with free radical scavenging activity (FRAP and DPPH assays) in both fresh and dry plant parts, suggesting that phenols are the main compounds responsible for the antioxidant activity. The root of H. isora L. was found rich in phenolics and antioxidant capacity indicating its strong potential for medicinal use, followed by fruit, leaf and bark.},
 year = {2014}
}

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TY - JOUR
T1 - A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.
AU - Amita Jain
AU - Rashmi Ranade
AU - Prem Pritam
AU - Neelu Joshi
AU - Sirisha Lakshmi Vavilala
AU - Ankita Jain
Y1 - 2014/10/30
PY - 2014
N1 - https://doi.org/10.11648/j.ajls.20140205.17
DO - 10.11648/j.ajls.20140205.17
T2 - American Journal of Life Sciences
JF - American Journal of Life Sciences
JO - American Journal of Life Sciences
SP - 292
EP - 302
PB - Science Publishing Group
SN - 2328-5737
UR - https://doi.org/10.11648/j.ajls.20140205.17
AB - Helicteres isora L., commonly known as Indian Screw Tree is a highly valued medicinal plant in South-East Asia. The various phytochemicals like phenols, flavonoids and other antioxidants that impart the medicinal properties in this plant, vary in their composition and concentration in different plant parts. In the present research, the total phenolic content, total flavonoids content and free radical scavenging activity (FRAP and DPPH assay) in fresh and dry sample extracts of leaf, bark, fruit and root of H. isora L., prepared in four different solvents (distilled water, ethanol, methanol and acetone) were studied, and their results compared using Pearson’s Correlation. The plant extracts were also subjected to RP-HPLC for detection and quantitation of naturally occurring phenolic compounds using six phenolic standards (Gallic acid, Vanillin, Catechol, Ferrulic acid, p-coumaric acid and Caffeic acid). The highest total phenolic content (7.22 mg/g GAE) and FRAP value (64.98 mg/g TE) were observed in aqueous dry root extract. The acetone extract of fresh leaf (57.08 mg/g of RE) was found richest in total flavonoids, while the methanolic extract of fresh fruit uniquely exhibited strong free radical scavenging activity as evidenced by the low IC50 value (34.37 mg/ml) in DPPH assay. The RP-HPLC analysis revealed that Catechol and Gallic acid were most abundantly found phenolic compounds in extracts of H. isora L. The total phenolic content showed strong positive correlation with free radical scavenging activity (FRAP and DPPH assays) in both fresh and dry plant parts, suggesting that phenols are the main compounds responsible for the antioxidant activity. The root of H. isora L. was found rich in phenolics and antioxidant capacity indicating its strong potential for medicinal use, followed by fruit, leaf and bark.
VL - 2
IS - 5
ER -

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Author Information

Amita Jain

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Rashmi Ranade

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Prem Pritam

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Neelu Joshi

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Sirisha Lakshmi Vavilala

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Ankita Jain

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India

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Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, et al. (2014). A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. American Journal of Life Sciences, 2(5), 292-302. https://doi.org/10.11648/j.ajls.20140205.17

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Amita Jain; Rashmi Ranade; Prem Pritam; Neelu Joshi; Sirisha Lakshmi Vavilala, et al. A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. Am. J. Life Sci. 2014, 2(5), 292-302. doi: 10.11648/j.ajls.20140205.17

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AMA Style

Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, et al. A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. Am J Life Sci. 2014;2(5):292-302. doi: 10.11648/j.ajls.20140205.17

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@article{10.11648/j.ajls.20140205.17,
  author = {Amita Jain and Rashmi Ranade and Prem Pritam and Neelu Joshi and Sirisha Lakshmi Vavilala and Ankita Jain},
  title = {A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {5},
  pages = {292-302},
  doi = {10.11648/j.ajls.20140205.17},
  url = {https://doi.org/10.11648/j.ajls.20140205.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140205.17},
  abstract = {Helicteres isora L., commonly known as Indian Screw Tree is a highly valued medicinal plant in South-East Asia. The various phytochemicals like phenols, flavonoids and other antioxidants that impart the medicinal properties in this plant, vary in their composition and concentration in different plant parts. In the present research, the total phenolic content, total flavonoids content and free radical scavenging activity (FRAP and DPPH assay) in fresh and dry sample extracts of leaf, bark, fruit and root of H. isora L., prepared in four different solvents (distilled water, ethanol, methanol and acetone) were studied, and their results compared using Pearson’s Correlation. The plant extracts were also subjected to RP-HPLC for detection and quantitation of naturally occurring phenolic compounds using six phenolic standards (Gallic acid, Vanillin, Catechol, Ferrulic acid, p-coumaric acid and Caffeic acid). The highest total phenolic content (7.22 mg/g GAE) and FRAP value (64.98 mg/g TE) were observed in aqueous dry root extract. The acetone extract of fresh leaf (57.08 mg/g of RE) was found richest in total flavonoids, while the methanolic extract of fresh fruit uniquely exhibited strong free radical scavenging activity as evidenced by the low IC50 value (34.37 mg/ml) in DPPH assay. The RP-HPLC analysis revealed that Catechol and Gallic acid were most abundantly found phenolic compounds in extracts of H. isora L. The total phenolic content showed strong positive correlation with free radical scavenging activity (FRAP and DPPH assays) in both fresh and dry plant parts, suggesting that phenols are the main compounds responsible for the antioxidant activity. The root of H. isora L. was found rich in phenolics and antioxidant capacity indicating its strong potential for medicinal use, followed by fruit, leaf and bark.},
 year = {2014}
}

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