Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease
American Journal of Life Sciences
Volume 2, Issue 6, December 2014, Pages: 400-405
Received: Dec. 15, 2014; Accepted: Dec. 23, 2014; Published: Jan. 6, 2015
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Authors
Olfat Mohamed Gab Allah, Oral Biology Department, Faculty of Dentistry, Tanta University, Tanta, Egypt
Mona Assem Abd-Elmotelb, Oral Biology Department, Faculty of Dentistry, Tanta University, Tanta, Egypt
Reda Gaber Saleh, Oral Biology Department, Faculty of Dentistry, Tanta University, Tanta, Egypt
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Abstract
Recent breakthrough studies gave a considerable interest on the pathogenesis of periodontal diseases, particularly, the critical role of cytokines. Cytokines have dual roles; they could be mediators of host defense against the microbial challenge, or mediators for periodontal tissue alteration. Osteopontin (OPN) is a glycosylated phosphoprotein, it functions as a Th 1 (T – helper 1) cytokine, promotes cell-mediated immune responses and plays a role in chronic inflammatory diseases. To assess OPN expression in dentogingival junction (DGJ) in chronic periodontitis, ten 13–16 months old, beagle dogs were used. Animals were distributed randomly into two equal groups: Group I: animals had healthy periodontium. Group II: chronic periodontitis was induced in animals. Four weeks after induction, dogs were sacrificed and specimens were prepared for immunohistochemical staining using OPN monoclonal antibody. Intensity of OPN reactions was recorded by image J and t-test was used to compare between the two groups. Healthy periodontium showed a mild OPN reaction. While, in chronic periodontitis, moderate to intense extracellular reaction was seen in DGJ. Also, sporadic nuclear OPN reactions of inflammatory cells were noticed in chronic periodontitis. T-test revealed a significant difference between normal and chronic periodontitis groups. In conclusion, OPN may play a role in chronic inflammation and can be considered as a biomarker of periodontal disease progression.
Keywords
OPN, Chronic Periodontitis, DGJ, Periodontium, Dogs
To cite this article
Olfat Mohamed Gab Allah, Mona Assem Abd-Elmotelb, Reda Gaber Saleh, Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease, American Journal of Life Sciences. Vol. 2, No. 6, 2014, pp. 400-405. doi: 10.11648/j.ajls.20140206.21
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