Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants
American Journal of Life Sciences
Volume 3, Issue 1, February 2015, Pages: 47-55
Received: Dec. 31, 2014; Accepted: Jan. 18, 2015; Published: Feb. 13, 2015
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Authors
Mohamed Maha Helmy, Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Gado Emad, Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia
El-Deeb Samir, Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Mostafa Helmy Mostafa, Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
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Abstract
One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups. Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris (teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG of R. solani on faba bean is AG4-HG1.
Keywords
Anastmosis Group, Rhizoctonia Solani AG4-HGI, PCR, Grouping of isolates, Phylogenetic Analysis
To cite this article
Mohamed Maha Helmy, Gado Emad, El-Deeb Samir, Mostafa Helmy Mostafa, Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants, American Journal of Life Sciences. Vol. 3, No. 1, 2015, pp. 47-55. doi: 10.11648/j.ajls.20150301.19
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