Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model

Walter Patterson; Devang Upadhyay; Sivanadane Mandjiny; Rebecca Bullard-Dillard; Meredith Storms; Michael Menefee; Leonard D. Holmes

doi:doi:10.11648/j.ajls.20150304.16

Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model

Walter Patterson, Devang Upadhyay, Sivanadane Mandjiny, Rebecca Bullard-Dillard, Meredith Storms, Michael Menefee, Leonard D. Holmes

Published in American Journal of Life Sciences (Volume 3, Issue 4)

Received: 16 June 2015 Accepted: 25 June 2015 Published: 8 July 2015

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Abstract

Though the intricate relationship between the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterial counterpart Photorhabdus luminescensis generally known; the role of bioluminescence produced by the bacterial symbiont is yet to be identified. The objective of this study was to determine if bacterial luminosity plays a crucial role in attraction of larval insect hosts. This study focused on bacterial bioluminescence produced from both in vitro and in vivo culturing of the bacterial symbiont. The obtained results portrays that the average distance between Galleria mellonellalarvae and the bacterial light source (P. luminescens)decreased in a linear fashion as a function of increasing intensities of luminosity; thereby supporting the hypothesis that bioluminescence offers a symbiotic role to attract insect host larvae.

Published in	American Journal of Life Sciences (Volume 3, Issue 4)
DOI	10.11648/j.ajls.20150304.16
Page(s)	290-294
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Photorhabdus Luminescens, Heterorhabditis Bacteriophora, Symbiosis, Bioluminescence, Biological Control Agent

References

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[2]	Ehlers RU (1992)Environmental and biotic factors influencing the control potential of entomopathogenic nematodes of the genus Steinernema and Heterorhabditis. In:Gommers F J, Mass PWT (eds) Nematology from Molecule to Ecosystem. European Society of Nematologists,UK, pp201-212
[3]	Ciche TA, Darby C, Ehlers RU,ForstS, Goodrich-Blair H (2006)Dangerous liaisons: The symbiosis of entomopathogenic nematodes and bacteria. Biol Control 38:22–46
[4]	Fischer-Le SauxM, Viallard V, Brunel B, Normand P, Boemare NE(1999)Polyphasic classification of the genus Photorhabdus and proposal of new taxa: P. luminescens subsp.luminescens subsp. nov.,P. luminescens subsp. akhurstii subsp. nov., P. luminescens subsp.laumondii subsp. nov., P. temperata sp. nov., P. temperata subsp. temperata subsp. nov.and P. asymbiotica sp. nov. Int J SystEvolMicr49:1645–1656
[5]	Hinchliffe S, Hares M, Dowling A, ffrench-Constant RH (2010)Insecticidal Toxins from the Photorhabdus and Xenorhabdusbacteria. Open Toxicol J 3:101-118
[6]	DabornPJ, Waterﬁeld N, Silva CP, Au CPY, Sharma S, ffrench-ConstantR H(2002)A single Photorhabdus gene, makes caterpillarsfloppy (mcf), allows Escherichia coli topersist within and kill insects. P NatlAcadSci USA 99:10742-10747
[7]	Hu K and Webster J (2000) Antibiotic production in relation to bacterial growth and nematode development in Photorhabdus Heterorhabditis infected Galleria mellonella larvae. FEMS Microbiology Letters 189:219-223
[8]	Pande C, Deng H, Rath P, Callender RH, Schwemer J(1989) Resonance raman spectroscopy of an ultraviolet-sensitive insect rhodopsin.Biochemistry-US 26:7426-7430
[9]	Welham P, Stekel D(2009)Mathematical model of the lux luminescence system in the terrestrial bacterium Photorhabdusluminescens. Mol Biosyst5:68-76
[10]	Briscoe A, Bernard G (2009) Eye shine and spectral tuning of long wavelength-sensitive rhodopsins: no evidence for red-sensitive photoreceptors among five Nymphalini butterfly species. J ExpBiol208:687-696
[11]	Inman III FL, Holmes LD (2012)The effects of trehalose on the bioluminescence and pigmentation of the phase I variant of Photorhabdus luminescens. J Life Sci6:119-129
[12]	Inman III FL, Singh S, Holmes LD (2012) Mass production of the beneficial nematode Heterorhabditis bacteriophora and its bacterial symbiont Photorhabdus luminescens. Indian J Microbiol 52:316-324
[13]	Zhou X, Kaya HK, Heungens K, Goodrich-Blair H (2002) Response of ants to a deterrent factor(s) produced by the symbiotic bacteria of entomopathogenic nematodes. Appl Environ Microbiol 68:6202-6209

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Walter Patterson, Devang Upadhyay, Sivanadane Mandjiny, Rebecca Bullard-Dillard, Meredith Storms, et al. (2015). Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model. American Journal of Life Sciences, 3(4), 290-294. https://doi.org/10.11648/j.ajls.20150304.16

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Walter Patterson; Devang Upadhyay; Sivanadane Mandjiny; Rebecca Bullard-Dillard; Meredith Storms, et al. Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model. Am. J. Life Sci. 2015, 3(4), 290-294. doi: 10.11648/j.ajls.20150304.16

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AMA Style

Walter Patterson, Devang Upadhyay, Sivanadane Mandjiny, Rebecca Bullard-Dillard, Meredith Storms, et al. Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model. Am J Life Sci. 2015;3(4):290-294. doi: 10.11648/j.ajls.20150304.16

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@article{10.11648/j.ajls.20150304.16,
  author = {Walter Patterson and Devang Upadhyay and Sivanadane Mandjiny and Rebecca Bullard-Dillard and Meredith Storms and Michael Menefee and Leonard D. Holmes},
  title = {Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {4},
  pages = {290-294},
  doi = {10.11648/j.ajls.20150304.16},
  url = {https://doi.org/10.11648/j.ajls.20150304.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150304.16},
  abstract = {Though the intricate relationship between the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterial counterpart Photorhabdus luminescensis generally known; the role of bioluminescence produced by the bacterial symbiont is yet to be identified. The objective of this study was to determine if bacterial luminosity plays a crucial role in attraction of larval insect hosts. This study focused on bacterial bioluminescence produced from both in vitro and in vivo culturing of the bacterial symbiont. The obtained results portrays that the average distance between Galleria mellonellalarvae and the bacterial light source (P. luminescens)decreased in a linear fashion as a function of increasing intensities of luminosity; thereby supporting the hypothesis that bioluminescence offers a symbiotic role to attract insect host larvae.},
 year = {2015}
}

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TY  - JOUR
T1  - Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model
AU  - Walter Patterson
AU  - Devang Upadhyay
AU  - Sivanadane Mandjiny
AU  - Rebecca Bullard-Dillard
AU  - Meredith Storms
AU  - Michael Menefee
AU  - Leonard D. Holmes
Y1  - 2015/07/08
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150304.16
DO  - 10.11648/j.ajls.20150304.16
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 290
EP  - 294
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150304.16
AB  - Though the intricate relationship between the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterial counterpart Photorhabdus luminescensis generally known; the role of bioluminescence produced by the bacterial symbiont is yet to be identified. The objective of this study was to determine if bacterial luminosity plays a crucial role in attraction of larval insect hosts. This study focused on bacterial bioluminescence produced from both in vitro and in vivo culturing of the bacterial symbiont. The obtained results portrays that the average distance between Galleria mellonellalarvae and the bacterial light source (P. luminescens)decreased in a linear fashion as a function of increasing intensities of luminosity; thereby supporting the hypothesis that bioluminescence offers a symbiotic role to attract insect host larvae.
VL  - 3
IS  - 4
ER  -

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Author Information

Walter Patterson

Sartorius Stedim Biotechnology Laboratory, Biotechnology Research and Training Center, The University of North Carolina at Pembroke, Pembroke, USA
Devang Upadhyay

Sartorius Stedim Biotechnology Laboratory, Biotechnology Research and Training Center, The University of North Carolina at Pembroke, Pembroke, USA
Sivanadane Mandjiny

Department of Chemistry and Physics, The University of North Carolina at Pembroke, Pembroke, USA
Rebecca Bullard-Dillard

School of Graduate Studies and Research, The University of North Carolina at Pembroke, Pembroke, USA
Meredith Storms

College of Arts & Sciences, The University of North Carolina at Pembroke, Pembroke, USA
Michael Menefee

Thomas Family Center for Entrepreneurship, The University of North Carolina at Pembroke, Pembroke, USA
Leonard D. Holmes

Sartorius Stedim Biotechnology Laboratory, Biotechnology Research and Training Center, The University of North Carolina at Pembroke, Pembroke, USA

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APA Style

Walter Patterson, Devang Upadhyay, Sivanadane Mandjiny, Rebecca Bullard-Dillard, Meredith Storms, et al. (2015). Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model. American Journal of Life Sciences, 3(4), 290-294. https://doi.org/10.11648/j.ajls.20150304.16

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ACS Style

Walter Patterson; Devang Upadhyay; Sivanadane Mandjiny; Rebecca Bullard-Dillard; Meredith Storms, et al. Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model. Am. J. Life Sci. 2015, 3(4), 290-294. doi: 10.11648/j.ajls.20150304.16

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AMA Style

Walter Patterson, Devang Upadhyay, Sivanadane Mandjiny, Rebecca Bullard-Dillard, Meredith Storms, et al. Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model. Am J Life Sci. 2015;3(4):290-294. doi: 10.11648/j.ajls.20150304.16

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@article{10.11648/j.ajls.20150304.16,
  author = {Walter Patterson and Devang Upadhyay and Sivanadane Mandjiny and Rebecca Bullard-Dillard and Meredith Storms and Michael Menefee and Leonard D. Holmes},
  title = {Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {4},
  pages = {290-294},
  doi = {10.11648/j.ajls.20150304.16},
  url = {https://doi.org/10.11648/j.ajls.20150304.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150304.16},
  abstract = {Though the intricate relationship between the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterial counterpart Photorhabdus luminescensis generally known; the role of bioluminescence produced by the bacterial symbiont is yet to be identified. The objective of this study was to determine if bacterial luminosity plays a crucial role in attraction of larval insect hosts. This study focused on bacterial bioluminescence produced from both in vitro and in vivo culturing of the bacterial symbiont. The obtained results portrays that the average distance between Galleria mellonellalarvae and the bacterial light source (P. luminescens)decreased in a linear fashion as a function of increasing intensities of luminosity; thereby supporting the hypothesis that bioluminescence offers a symbiotic role to attract insect host larvae.},
 year = {2015}
}

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TY  - JOUR
T1  - Attractant Role of Bacterial Bioluminescence of Photorhabdusluminescenson a Galleria mellonella Model
AU  - Walter Patterson
AU  - Devang Upadhyay
AU  - Sivanadane Mandjiny
AU  - Rebecca Bullard-Dillard
AU  - Meredith Storms
AU  - Michael Menefee
AU  - Leonard D. Holmes
Y1  - 2015/07/08
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150304.16
DO  - 10.11648/j.ajls.20150304.16
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 290
EP  - 294
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150304.16
AB  - Though the intricate relationship between the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterial counterpart Photorhabdus luminescensis generally known; the role of bioluminescence produced by the bacterial symbiont is yet to be identified. The objective of this study was to determine if bacterial luminosity plays a crucial role in attraction of larval insect hosts. This study focused on bacterial bioluminescence produced from both in vitro and in vivo culturing of the bacterial symbiont. The obtained results portrays that the average distance between Galleria mellonellalarvae and the bacterial light source (P. luminescens)decreased in a linear fashion as a function of increasing intensities of luminosity; thereby supporting the hypothesis that bioluminescence offers a symbiotic role to attract insect host larvae.
VL  - 3
IS  - 4
ER  -

Copy | Download