In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia
American Journal of Life Sciences
Volume 3, Issue 5, October 2015, Pages: 365-368
Received: Jul. 30, 2015; Accepted: Aug. 6, 2015; Published: Sep. 2, 2015
Views 3372      Downloads 91
Teklit Gebregiorgis Amabye, Department of Chemistry, College of Natural and Computational Science, Mekelle University, Mekelle, Ethiopia
Article Tools
Follow on us
To examine the in vitro anti bacterial activities of the ethanol extract of fresh leaves of Alliumcepa (onion) and to determine and quantify the phenol compounds of the investigated plantparts. This study was drifting out at the Mekelle university department chemistry and Adigrat pharmaceutical industry, fromMarch2015toApril 2015. Clinical strains of Streptococcus pneumoniae and ethanol extracts of the plant species was used for the antimicrobial study. Thirty grams of the sample was ground, filtrated, and each filtrate mixed with 100 ml ethanol and placed in a shaker for 48 hours. Theethanol was evaporated from the sample, weighed, and subjected to an antibacterial activity test using the agar diffusion method. The high- performance liquid chromatography was used to identify and quantify the phenols extracts of investigated samples. Ethanol extract of the investigated plant parts showed antibacterial activities against different pathogenicbacteria. Leaf extracts of Alliumcepashowed the highest antibacterial activity and contains more phenols. Theethanol extract of the tested plants could be considered as analternative source of new antibacterialdrugs.
HPLC, Antimicrobial Empire, Streptococcus Pneumoniae, Medicinal Plants
To cite this article
Teklit Gebregiorgis Amabye, In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia, American Journal of Life Sciences. Vol. 3, No. 5, 2015, pp. 365-368. doi: 10.11648/j.ajls.20150305.15
Ying QH, Zhang JJ, Yang L, Ding JL, Song XH, Du W, etal. Antimicrobial resistance, serotypes and genotypes of Streptococcus pneumoniae isolates associated with ocular infection in China. Afr J Microbiol Res 2012; 6(1):160-164.
PatrickRM,KentR.MedicalMicrobiology.4thed.StLouis,USA:MosbyInc;2002, p.232-234.
Tomoka N, Takashi A, Hiromu T, Yuka I, Hiroko M, MunekazuI, et al. Antibacterial activity of extracts preparated from tropical and subtropical plants onmethicillin-resistant Staphylococcus aureus. J Health Sci 2002; 48:273-276.
Pillar CM, Draghi DC, Sheehan DJ, Sahm DF. Prevalence of multidrug-resistant, methicillin-resistant Staphylococcus aureus in the United States: findings of the stratified analysisofthe2004to2005LEADERSurveillancePrograms.DiagnMicrobiolInfect Dis 2008; 60:221-224.
GottliebOR,BorinMR,deBritoNR.Integrationofethnobotanyandphytochemistry. Dream or reality? Phytochemistry 2002; 60: 145-152.
Narod FB, Gurib-Fakim A, Subratty AH. Biological investigations into Antidesmamadagascariense Lam. (Euphorbiaceae), Faujasiopsisflexuosa (Lam.) C. Jeffery (Asteraceae), Toddaliaasiatica (L.) Lam. and Veprislanceolata (Lam.) G. Don (Rutaceae). Journal of Cell and Molecular Biology 2004; 3:15-21.
Benkeblia N, “Antimicrobial activity of essential oil extracts of various onions (Alliumcepa) and garlic (Allium sativum)” Lebensm.-Wiss. u.-Technol, 2004; 37:263–268.
Shandavi C.B hangale, Vikas V. Patil, Vijay R. Pati. “Anti-bacterial Activity of Ficus
Bengalensis Linn. Bark on Actinomycesviscosus”, International Journal of Pharmaceutical Sciences, Vol.2 (1), 2010, 39-43.
Tasioula-Margari M, Okogeri O. Simultaneous determination of phenolics compounds and tocopherols in virgin olive oil using HPLC and UV detection. Food Chemistry 2001;74: 369-375.
Singh UP, Suman A, Sharma M, Singh JN, Singh A, Maurya S.HPL Canalysisofthe phenolicprofilesin different parts of Chilli (Capsicumannum) and Okra (Abelmoschusesculentus L.) Moench. The Internet Journal of Alternative Medicine 2008; 5:2.
Saad A. Alamri, MSc, PhD, Mahmoud F. Moustafa, MSc, PhD Antimicrobialproperties of 3 medicinal plants from Saudi Arabia against some clinical isolates of bacteria. Saudi Med J 2012; Vol. 33 (3):272-27.
Madhuri S, Pandey G. Some anticancer medicinal plants of foreign origin. Current Science 2009; 96:779-783.
Lampe JW. Spicing up a vegetarian diet: chemo preventive effects of phytochemicals. Am J Clin Nutr 2003; 78: 579S-583S.
Thankamma L. Hevea latex as wound healer and pain killer. Current Science 2003;84: 971-972.
NarendraN,GauravP,LokeshD.NaveenKJ.Woundhealingactivityoflatexof Calotropis gigantean. International Journal of Pharmaceutical Science 2009; 1:176-181.
Sequeira BJ, Vital MJ, Pohlit AM, Pararols IC, Caúper GS. Antibacterialand antifungal activity of extracts and exudates of the Amazonian medicinal treeHimatanthusarticulatus(Vahl)Woodson(commonname:sucuba).MemInstOswaldoCruz.2009; 104: 659-661.
Tasnia Ahmed, Nusrat Jahan Urmi, Md. Sakil Munna, Kamal Kanta Das, Mrityunjoy Acharjee, M Majibur Rahman, Rashed Noor. Assessment of Microbiological Proliferation and in Vitro Demonstration of the Antimicrobial Activity of the Commonly Available Salad.
Vegetables within Dhaka Metropolis, Bangladesh. American Journal of Agriculture and Forestry. Vol. 2, No. 3, 2014, pp.55-60. doi: 10.11648/j.ajaf.20140203.11
Nguyen Thi Dong Phuong, Dang Minh Nhat. Antimicrobial Activities of the Extracts from Bark of Ironwood (Mesua ferrea) and their Role in Production of Traditional Sugar Palm Wine. Journal of Food and Nutrition Sciences. Special Issue: Food Processing and Food Quality. Vol. 3, No. 1-2, 2015, pp. 65-69. doi: 10.11648/j.jfns.s.2015030102.22
Abdelkarim Guaâdaoui, Fatma Bouhtit, Abdellah Hamal, the Preventive Approach of Biocompounactives (1): A Review in Recent Advances in Common Vegetables and Legumes, International Journal of Nutrition and Food Sciences. Vol. 4, No. 1, 2015, pp. 89-102. doi: 10.11648/j.ijnfs.20150401.23.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186