Prevalence of Beta-Thalassemia Trait Among Students of the University College of Science and Technology-Palestine
American Journal of Life Sciences
Volume 4, Issue 2, April 2016, Pages: 40-46
Received: Mar. 22, 2016;
Accepted: Mar. 31, 2016;
Published: Apr. 15, 2016
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Lamia'a Sobhi Saqer, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
Mona Ziad Almasri, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
Shahed Awad Almasri, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
Zahraa Akram Almasri, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
Thalassemias are a group of recessively inherited genetic disorders mostly common in the Mediterranean, the equatorial and near equatorial regions of Africa and Asia. Large number of mutations cause abnormal globin gene expression and result in complete absence or reduction of globin chain synthesis which lead to thalassemia. β-thalassemia is the result of deficient or absent synthesis of β-globin chains, leading to excess α chains. This study was conducted in order to determine the prevalence of β-thalassemia trait among students of University College of Science and Technology (UCST) in Khan Younis, Gaza Strip-Palestine. Allele Specific PCR (ASPCR) was used to determine the intervening sequence IVSI-6 (T →C) and IVSI-110 (G →A) mutations. The study population consisted of 348 subjects recruited from the UCST (144 males: 41% and 204 females: 59%). Blood samples were collected in EDTA tube for CBC. Mentzer index was calculated for all samples. Blood film was done and stained using Giemza stain. DNA was isolated from 12 samples that had normal RBCs and low MCV and whose Mentzer index was >13. These samples were subjected to Allele Specific PCR in order to detect IVSI-6 (T →C) and IVSI-110 (G →A) mutations. The hemoglobin level in females was found to be about 20% less than the level recorded in males (11.40±1.01 vs 14.30±0.79 g/dl). The results also revealed that there were significant differences in all measured CBC parameters and indices between males and females except that for WBC, RBC, MCHC and PLT. IVSI-6 (T →C) mutation was detected only in two samples and both were heterozygous. IVSI-110 (G →A) mutation was not detected in this study. The present results showed that the case of β-thalassemia carrier have normal RBC, MCH, Hb and normal Mentzer index which could be missed in routine screening test.
Lamia'a Sobhi Saqer,
Mona Ziad Almasri,
Shahed Awad Almasri,
Zahraa Akram Almasri,
Prevalence of Beta-Thalassemia Trait Among Students of the University College of Science and Technology-Palestine, American Journal of Life Sciences.
Vol. 4, No. 2,
2016, pp. 40-46.
D. Weatherall and J. Clegg, “Inherited hemoglobin disorders: an increasing global health problem”, Bull World Health Organ, 79(8), pp 704-712, 2001.
M. Honarbakhshi and H. Rahmanpour, “The prevalence of thalassemia minor and recognize carriers for prevention of thalassemia in Tarem Oolia region”, Scientific Journal of Zanjan Medical University, 1377(23), pp 21-27, 2012.
S. Mok, M Imwong. and M. J. Mackinnon, “Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription”, BMC Genomics, 12(3), pp 391-405, 2011.
F. Collins and S. Weissman, “The molecular genetics of human hemoglobin”, Progress in nucleic acid research and molecular biology, 31(8), pp 315-421, 1984.
A. Cao and R. Galanello, “Beta-thalassemia”, Genetics In Medicine, 12(2), pp 61-76, 2010.
P. Lahiry, S. Al-Attar and R. Hegele, “Understanding beta-thalassemia with focus on the Indian Subcontinent and the Middle East”, Hematology Journal, 2(1), pp 5-13, 2008.
S. L. Thein, “Genetic modifiers of β-thalassemia”, Hematologica, 124(3), pp 649-60, 2005.
M. Valko, D. Leibfritz and J. Moncol, “Free radicals and antioxidants in normal physiological functions and human disease”, The International Journal of Biochemistry & Cell Biology, 39(1), pp 44-84, 2007.
A. Ceriello, “Possible role of oxidative stress in the pathogenesis of hypertension”, Review, Diabetes Care, 31(2), pp 181-184, 2008.
R. Galanello, A. Mosca, R. Paleari, G. Ivaldi, P. Giordano, “The role of haemoglobin A2 testing in the diagnosis of thalassemias and related haemoglobinopathies”, Journal of Clinical Pathology, 62(1), pp 13-20, 2009.
D. Filon, V. Oron, R. Shawa, E. Elborno, K. Najjar, T. Tulchinsky, E. Rachmilewitz, D. Rund, A. Oppenheim, “Spectrum of β-thalassemia mutations in the Gaza Area”, Human Mutation 5, pp 351-353, 1995.
C. R. Newton, A. Graham and L. E. Heptinstall, “Analysis of any point mutation in DNA, the amplification refractory mutation system (ARMS)”, Nucleic Acids Research,17(7), pp 2503-2519, 1989.
M. Sirdah, Y. Bilto, S. El-Jabour and K. Najjar, “Screening secondary school students in the Gaza Strip for β-thalassemia trait, Clinical and laboratory”, Haematology, 20(5), pp 279-283, 1998.
Z. Harara, “Occurrence of hereditary hemochromatosis among β-thalassemia intermediate and β-thalassemia minor subjects in Gaza Strip - Palestine”, MSc thesis, Islamic University of Gaza, 2006.
S. Elhams, “Immunological assessment of B-thalassemic major children aged 5-12 years old attending Abd El-Aziz El-Rantisy Hospital in Gaza Strip”, MSc Thesis, Islamic University of Gaza, Palestine, 2010.
Z. Habib, “Hematological criteria in hemoglobinopathy-free adult Egyptians” Hereditas, 95(23), pp 331-332, 2009.
S. Al-Sweedan, M. Alhaj, “The effect of low altitude on blood count parameters from the department of Pediatrics, Jordan University of Science and Technology, Irbid, Jordan”, Hematology/Oncology and Stem Cell Therapy, 5(3), pp 158-161, 2012.
L. Dennis, M. Tein, T. Lau, C. Haines, T. Leung, P. Poon, J. Wainscoat, P. Johnson, A. Chang, N. Hjelm, “Quantitative analysis of fetal DNA in maternal plasma and serum: Implications for noninvasive prenatal diagnosis”, The American Journal of Human Genetics, 62(4), pp 768–775, 1998.
L. Zahed, “The spectrum of α-thalassemia mutations in the Arab populations”, Biomedicine and Biotechnology Journal, 1(3), pp 129-132, 2001.
S. El-Gawhary, S. El-Shafie, M. Niazi, M. Aziz, A. El-Beshlawy, “Study of β-thalassemia mutations using the polymerase chain reaction-amplification refractory mutation system and direct DNA sequencing techniques in a group of Egyptian thalassemia patients”, Hemoglobin, 31(1), pp 63-69, 2007.
H. Darwish, F. El-Khatib, S. Ayesh, “Spectrum of β-globin gene mutations among thalassemia patients in the West Bank region of Palestine”, Hemoglobin, 29(2), pp 119-132, 2005.