Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase
American Journal of Life Sciences
Volume 4, Issue 2, April 2016, Pages: 47-50
Received: Feb. 5, 2016; Accepted: Feb. 27, 2016; Published: May 6, 2016
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David Morakinyo Sanni, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
Oluwasegun Victor Omotoyinbo, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
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This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.
Datura metel, Tyrosinase, Inhibition
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David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo, Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase, American Journal of Life Sciences. Vol. 4, No. 2, 2016, pp. 47-50. doi: 10.11648/j.ajls.20160402.15
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