American Journal of Life Sciences

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Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth

Received: 30 April 2016    Accepted: 01 June 2016    Published: 17 June 2016
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Abstract

Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.

DOI 10.11648/j.ajls.20160403.13
Published in American Journal of Life Sciences (Volume 4, Issue 3, June 2016)
Page(s) 82-86
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Nile Tilapia, Myostatin Gene, Polymorphism, SNPs, RFLP

References
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[3] Xu C, Wu G, Zohar Y, Du SJ. 2003. Analysis of myostatin gene structure, expression and function in zebrafish. J. Exp. Biol. 206: 4067–4079.
[4] Ye HQ, Chen SL, Sha, ZX, Liu Y. 2007. Molecular cloning and expression analysis of the myostatin gene in sea perch (Lateolabrax japonicus). Marine Biotechnol. 9: 262–272.
[5] De Santis C, Evans BS, Smith-Keune C, Jerry DR. 2008. Molecular characterization, tissue expression and sequence variability of the barramundi (Lates calcarifer) myostatin gene. BMC Genomics 9: 1–15.
[6] Zhong QW, Zhang QQ, Chen YJ, Sun YY, Qi J, Wang ZG, Li S., Li C. Lan, X. 2008. The isolation and characterization of myostatin gene in Japanese flounder (Paralichthys olivaceus): ubiquitous tissue expression and developmental specific regulation. Aquaculture. 280: 247–255.
[7] Gabillard JC, Biga PR, Rescan PY, Seiliez, I. 2013. Revisiting the paradigm of myostatin in vertebrates: insights from fishes. General comp Endocrinol. 194: 45–54.
[8] Rodgers BD, Weber GM. 2001. Sequence conservation among fish myostatin orthologues and the characterization of two additional cDNA clones from Morone saxatilis and Morone americana. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 129: 597–603.
[9] Wang XL, Meng XY, Song B, Qiu XM, Liu HY. SNPs in the myostatin gene of the mollusk Chlamys farreri: association with growth traits. Comp Biochem Physiol B 2010; 155: 327¨C 330.
[10] Tang YK, Li JL, Yu JH, Chen XF and Li HX. 2010. Genetic structure of MSTN and association between its polymorphisms and growth traits in genetically improved farmed tilapia (GIFT), J Fish Sci. China 17: 44¨C 51.
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[14] Li H, Fan J, Liu H, Yang Q, Mu G and He C. 2012. Characterization of a myostatin gene (MSTN 1) from spotted halibut (Verasper variegatus) and association between its promoter polymorphism and individual growth performance. Comp. Biochem. Physiol. Part. B 161: 315–322.
[15] Rodgers BD and Garikipati DK. 2008. Clinical, agricultural, and evolutionary biology of myostatin: a comparative review. Endocrine Rev. 29: 513–534.
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Author Information
  • Biotechnology Department, Animal Health Research Institute-Kafer El-Shik, Egypt

  • Biotechnology Department, Animal Health Research Institute-Kafer El-Shik, Egypt

  • Animal Wealth and Animal Husbandry Department, Faculty of Veterinary Medicine, Alexandria University, Egypt

  • Department of Physiology, Faculty of Veterinary Medicine, Kafer El Sheikh University, Egypt

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    Nasema A. Elkatatny, Zizy I. Elbialy, Abeer F. El-Nahas, Shawky Mahmoud. (2016). Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. American Journal of Life Sciences, 4(3), 82-86. https://doi.org/10.11648/j.ajls.20160403.13

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    ACS Style

    Nasema A. Elkatatny; Zizy I. Elbialy; Abeer F. El-Nahas; Shawky Mahmoud. Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. Am. J. Life Sci. 2016, 4(3), 82-86. doi: 10.11648/j.ajls.20160403.13

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    AMA Style

    Nasema A. Elkatatny, Zizy I. Elbialy, Abeer F. El-Nahas, Shawky Mahmoud. Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. Am J Life Sci. 2016;4(3):82-86. doi: 10.11648/j.ajls.20160403.13

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  • @article{10.11648/j.ajls.20160403.13,
      author = {Nasema A. Elkatatny and Zizy I. Elbialy and Abeer F. El-Nahas and Shawky Mahmoud},
      title = {Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth},
      journal = {American Journal of Life Sciences},
      volume = {4},
      number = {3},
      pages = {82-86},
      doi = {10.11648/j.ajls.20160403.13},
      url = {https://doi.org/10.11648/j.ajls.20160403.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20160403.13},
      abstract = {Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth
    AU  - Nasema A. Elkatatny
    AU  - Zizy I. Elbialy
    AU  - Abeer F. El-Nahas
    AU  - Shawky Mahmoud
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    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
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    EP  - 86
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20160403.13
    AB  - Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.
    VL  - 4
    IS  - 3
    ER  - 

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