Gap Filling for a Human MHC Haplotype Sequence
American Journal of Life Sciences
Volume 4, Issue 6, December 2016, Pages: 146-151
Received: Nov. 29, 2016; Published: Dec. 1, 2016
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Authors
Yuanwei Zhang, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China; BGI-Shenzhen, Shenzhen, China
Tao Zhang, BGI-Shenzhen, Shenzhen, China
Zuhong Lu, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
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Abstract
The major histocompatibility complex (MHC) is recognized as the most variable region in the human genome and has susceptibility to > 100 diseases. We constructed a complete MHC haplotype sequence of MCF cell line by gap filling based on whole genome sequencing (WGS) data. Gaps spanning ~ 1 Mb were filled and 31 genes were annotated in these gaps. This sequence could be used as reference to identify disease associations within this haplotype or similar haplotypes. The method for gap filling can be applied to other MHC haplotypes or other genomic region.
Keywords
Gap Filling, MHC, Haplotype, WGS
To cite this article
Yuanwei Zhang, Tao Zhang, Zuhong Lu, Gap Filling for a Human MHC Haplotype Sequence, American Journal of Life Sciences. Vol. 4, No. 6, 2016, pp. 146-151. doi: 10.11648/j.ajls.20160406.12
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