An Impact of the Trivedi Effect® - Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Mouse Splenocytes
American Journal of Life Sciences
Volume 4, Issue 6, December 2016, Pages: 164-174
Received: Nov. 18, 2016; Accepted: Nov. 28, 2016; Published: Dec. 8, 2016
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Ariadne Esmene Afaganis, Trivedi Global, Inc., Henderson, Nevada, USA
Barbara Marie Bader, Trivedi Global, Inc., Henderson, Nevada, USA
Brian A. Weekes, Trivedi Global, Inc., Henderson, Nevada, USA
Daphne Luisa Dumas, Trivedi Global, Inc., Henderson, Nevada, USA
Denise Marie Fiedler, Trivedi Global, Inc., Henderson, Nevada, USA
Dennille Mellesia Smith, Trivedi Global, Inc., Henderson, Nevada, USA
Desi Pano, Trivedi Global, Inc., Henderson, Nevada, USA
Donna Felice Galla, Trivedi Global, Inc., Henderson, Nevada, USA
Donna Maria Alija, Trivedi Global, Inc., Henderson, Nevada, USA
Elaine Barbara Mullins, Trivedi Global, Inc., Henderson, Nevada, USA
Elaine M. Scorza, Trivedi Global, Inc., Henderson, Nevada, USA
Ellia O'Donnell, Trivedi Global, Inc., Henderson, Nevada, USA
Fabio Massimo Paciucci, Trivedi Global, Inc., Henderson, Nevada, USA
Frances Goodman Warlick, Trivedi Global, Inc., Henderson, Nevada, USA
Haddon Norman Salt, Trivedi Global, Inc., Henderson, Nevada, USA
Inthirani Arul, Trivedi Global, Inc., Henderson, Nevada, USA
Jacqueline Y. Andrews, Trivedi Global, Inc., Henderson, Nevada, USA
James Jay McLeran, Trivedi Global, Inc., Henderson, Nevada, USA
James Stephen Burnett, Trivedi Global, Inc., Henderson, Nevada, USA
Jean Caroline White, Trivedi Global, Inc., Henderson, Nevada, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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Abstract
Due to the increased popularity of herbomineral preparations in the healthcare sector, a new proprietary herbomineral formulation was formulated consisting of ashwagandha root extract and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The objective of the study was to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine splenocyte cells. The herbomineral formulation was divided into two parts; one defined as the control, while the other part was treated with the Biofield Energy Healing Treatment performed from a remote distance by twenty renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The splenocyte cells were exposed to test formulations at concentration from 0.00001053 to 10.53 µg/mL and were analyzed after 48 hours for cell viability using MTT assay. The expression of the cytokines (TNF-α, IFN-γ, IL-1β, and MIP-1α) was determined using ELISA assay. The cell viability data showed that all the tested concentration ranges were found to be safe with percentage cell viability at more than 80%. Further, TNF-α expression was significantly inhibited in the Biofield Treated test formulation group with respect to the vehicle control, while at 0.001053 and 0.1053 µg/mL, the expression was suppressed by 1.70% and 8.16%, respectively in the Biofield Treated test formulation compared to the untreated formulation. However, a significant immunosuppression was reported in IFN-γ expression at 0.00001053, 0.0001053, 0.01053, 0.1053, and 1.053 µg/mL by 12.63%, 2.31%, 8.31%, 9.15%, and 7.86%, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The MIP-1α expression was inhibited by 8.31%, 21.53%, and 8.70% at 0.0001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated formulation compared with the untreated test formulation. However, IL-1β expression was significantly suppressed by 19.72% at concentration 0.00001053 µg/mL in the Biofield Treated test formulation compared with the untreated test formulation. Thus, the down-regulation of tested cytokines and chemokines in the Biofield Energy Healing test formulation might be applicable for controlling acute and chronic inflammation in many clinical diseases. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory activity of the test formulation, which can be useful against autoimmune disorders. Biofield Treated Test formulation may also be useful in anti-aging, anti-inflammatory, stress management and in preventing immune-mediated tissue damage in organ transplants by improving overall health and quality of life.
Keywords
Biofield Energy Healing Treatment, The Trivedi Effect®, Herbomineral Formulation, Immune-Modulation, Pro-inflammatory Cytokines, Splenocytes
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Mayank Gangwar, Snehasis Jana, An Impact of the Trivedi Effect® - Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Mouse Splenocytes, American Journal of Life Sciences. Vol. 4, No. 6, 2016, pp. 164-174. doi: 10.11648/j.ajls.20160406.14
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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