Overall Skin Health Potential of the Biofield Energy Healing Based Herbomineral Formulation Using Various Skin Parameters
American Journal of Life Sciences
Volume 5, Issue 2, April 2017, Pages: 65-74
Received: Mar. 30, 2017; Accepted: Apr. 19, 2017; Published: May 8, 2017
Views 1592      Downloads 39
Authors
Janice Patricia Kinney, Trivedi Global, Inc., Henderson, USA
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, USA
Alice Branton, Trivedi Global, Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, USA
Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Article Tools
Follow on us
Abstract
The aim of the present study was to evaluate the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against various skin health parameters using three cell lines i.e., HFF-1, HaCaT, and B16-F10. The various study parameters viz. collagen, elastin, hyaluronic acid, melanin, cell viability against UV-B induced stress, and wound healing were evaluated. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Janice Patricia Kinney and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 70% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The collagen synthesis was significantly (p≤0.001) increased by 22.42% and 17.48% in the UT-DMEM + BT-Test formulation group at 0.63 and 2.5 µg/mL, respectively in relation to the UT-DMEM + UT-Test formulation group. The Elastin level was significantly (p≤0.001) increased by 28.41% in the BT-DMEM + BT-Test formulation group at 5 µg/mL compared to the untreated group. Hyaluronic acid at 0.63 µg/mL was increased significantly by 15.90% in the BT-DMEM + BT-Test formulation group compared to the untreated group. The level of melanin was reduced significantly by 9.25% and 7.26% in the BT-DMEM + BT-Test formulation group at 0.013 and 0.063 µg/mL, respectively in relation to the untreated group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased significantly by 17.88%, 20.10%, and 25.77% in the BT-DMEM + BT-Test formulation group at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 and HaCaT cells compared to the UT-DMEM + UT-Test formulation. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better responses compared to the untreated medium and test formulation with respect to the tested skin health parameters. Therefore, the Biofield Energy Healing and the Treated test formulation could be developed as an effective cosmetic product to protect and treat the various skin problems including infection, photosensitivity, erythema, contact dermatitis, seborrheic dermatitis, athlete's foot, psoriasis, erythema, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color, etc.
Keywords
Consciousness Energy Healing, The Trivedi Effect®, Skin Protection, HFF-1; B16-F10, HaCaT, Scratch Assay, Extracellular Matrix
To cite this article
Janice Patricia Kinney, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana, Overall Skin Health Potential of the Biofield Energy Healing Based Herbomineral Formulation Using Various Skin Parameters, American Journal of Life Sciences. Vol. 5, No. 2, 2017, pp. 65-74. doi: 10.11648/j.ajls.20170502.15
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Tabassum N, Hamdani M (2014) Plants used to treat skin diseases. Pharmacogn Rev 8: 52-60.
[2]
Goyal RK (2005) Investigation of cellular and molecular mechanisms for anti-diabetic drugs with special reference to Unani and Ayurvedic herbal medicines. In: Traditional system of medicine, Abdin, M. Z. and Y. P. Abrol (Eds.). Narosa Publishing House, New Delhi.
[3]
WHO (1993) Research Guideline for Evaluating the Safety and Efficacy of Herbal Medicines. World Health Organization, Manila, Philippines.
[4]
Park K (2015) Role of micronutrients in skin health and function. Biomol Ther (Seoul) 23: 207-217.
[5]
McDaniel S, Goldman GD (2002) Consequences of using escharotic agents as primary treatment for nonmelanoma skin cancer. Arch Dermatol 138: 1593-1596.
[6]
Dziaman T, Huzarski T, Gackowski D, Rozalski R, Siomek A, Szpila A, Guz J, Lubinski J, Wasowicz W, Roszkowski K, Olinski R (2009) Selenium supplementation reduced oxidative DNA damage in adnexectomized BRCA1 mutations carriers. Cancer Epidemiol Biomarkers Prev 18: 2923-2928.
[7]
Spallholz JE (2001) Selenium and the prevention of cancer Part II: Mechanisms for the carcinostatic activitiy of Se compounds. The Bulletin of Selenium-Tellurium Development Association. 2001: 12.
[8]
Combs GF Jr., Clark LC, Turnbull BW (1997) Reduction of cancer mortality and incidence by selenium supplementation. Med Klin (Munich) 92: 42-45.
[9]
Combs GF Jr., Clark LC, Turnbull BW (1997) Reduction of cancer risk with an oral supplement of selenium. Biomed Environ Sci 10: 227-234.
[10]
Institute of Medicine (IOM), (2001). Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc. Food and Nutrition Board. Washington, DC, USA (Ed.), National Academy Press, Washington, DC, pp. 420–441.
[11]
Kisker C, Schindelin H, Rees DC (1997) Molybdenum-cofactor-containing enzymes: Structure and mechanism. Annu Rev Biochem 66: 233-267.
[12]
Boyera N, Galey I, Bernard BA (1998) Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts. Int J Cosmet Sci 20: 151-158.
[13]
Sugiyama Y, Kawakishi S, Osawa T (1996) Involvement of the β-diketone moiety in the antioxidant mechanism of tetrahydrocurcuminoids. Biochem Pharmacol 52: 519-525.
[14]
Lai CS, Wu JC, Yu SF, Badmaev V, Nagabhushanam K, Ho CT, Pan MH (2011) Tetrahydrocurcumin is more effective than curcumin in preventing azoxymethane-induced colon carcinogenesis. Mol Nutr Food Res 55: 1819-1828.
[15]
Hashim P (2011) Centella asiatica in food and beverage applications and its potential antioxidant and neuroprotective effect. Int Food Res J 18: 1215-1222.
[16]
Hong SS, Kim JH, Shim CK (2005) Advance formulation and pharmacological activity of hydrogel of titrated extract of Centella asiatica. Arch Pharma Res 28: 502-508.
[17]
Shetty BS, Udupa AL, Somayaji SN (2006). Effect of Centella asiatica L. on normal and dexamethasone suppressed wound healing in Wistar Albino rats. Int J Low Extrem Wounds 5: 137-143.
[18]
Hammerschlag R, Jain S, Baldwin AL, Gronowicz G, Lutgendorf SK, Oschman JL, Yount GL. (2012) Biofield research: A roundtable discussion of scientific and methodological issues. J Altern Complement Med 18: 1081-1086.
[19]
Movaffaghi Z, Farsi M (2009) Biofield therapies: Biophysical basis and biological regulations? Complement Ther Clin Pract 15: 35-37.
[20]
Sun Y, Wang C, Dai J (2010) Biophotons as neural communication signals demonstrated by in situ biophoton autography. Photochem Photobiol Sci 9: 315-322.
[21]
Yount G, Patil S, Dave U, Alves-dos-Santos L, Gon K, Arauz R, and Rachlin K (2013) Evaluation of biofield treatment dose and distance in a model of cancer cell death. J Altern Complement Med 19: 124-127.
[22]
Garland SN, Valentine D, Desai K, Li S, Langer C, Evans T, Mao JJ (2013) Complementary and alternative medicine use and benefit finding among cancer patients. J Altern Complement Med 19: 876-881.
[23]
Trivedi MK, Tallapragada RM (2008) A transcendental to changing metal powder characteristics. Met Powder Rep 63: 22-28, 31.
[24]
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage 4: 161.
[25]
Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bull Mater Sci 32: 471-479.
[26]
Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.
[27]
Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 83: 138-143.
[28]
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.
[29]
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 203-206.
[30]
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An effect of biofield treatment on multidrug-resistant Burkholderia cepacia: A multihost pathogen. J Trop Dis 3: 167.
[31]
Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.
[32]
Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
[33]
Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity (ISO 10993-5:2009), I.S.EN ISO, 10993-5: 2009.
[34]
Junquiera LC, Junqueira LC, Brentani RR (1979) A simple and sensitive method for the quantitative estimation of collagen. Anal Biochem 94: 96-99.
[35]
Hahn MS, Kobler JB, Starcher BC, Zeitels SM, Langer R (2006) Quantitative and comparative studies of the vocal fold extracellular matrix. I: Elastic fibers and hyaluronic acid. Ann Otol Rhinol Laryngol 115: 156-164.
[36]
Zhang L, Yoshida T, Kuroiwa Y (1992) Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin. Life Sci 51: 17-24.
[37]
Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I (2009) Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol 126: 463-467.
[38]
Wen KC, Shih IC, Hu JC, Liao ST, Su TW, Chiang HM (2011) Inhibitory effects of Terminalia catappa on UV-B-induced photodamage in fibroblast cell line. Evid Based Complement Alternat Med 2011: 904532.
[39]
Rozario T, DeSimone DW (2010) The extracellular matrix in development and morphogenesis: A dynamic view. Dev Biol 341: 126-140.
[40]
Cardinale GJ, Udenfriend S (1974) Prolyl hydroxylase. Adv Enzymol Relat Areas Mol Biol 41: 245-300.
[41]
Yin L, Morita A, Tsuji T (2001) Skin aging induced by ultraviolet exposure and tobacco smoking: Evidence from epidemiological and molecular studies. Photodermatol Photoimmunol Photomed 17: 178-183.
[42]
Castelo-Branco C, Figueras F, Martínez de Osaba MJ, Vanrell JA (1998) Facial wrinkling in postmenopausal women. Effects of smoking status and hormone replacement therapy. Maturitas 29: 75-86.
[43]
Youn CS, Kwon OS, Won CH, Hwang EJ, Park BJ, Eun HC, Chung JH (2003) Effect of pregnancy and menopause on facial wrinkling in women. Acta Derm Venereol 83: 419-424.
[44]
Contet-Audonneau JL, Jeanmaire C, Pauly G (1999) A histological study of human wrinkle structures: comparison between sun-exposed areas of the face, with or without wrinkles, and sun-protected areas. Br J Dermatol 140: 1038-1047.
[45]
Brincat M, Moniz CJ, Studd JW, Darby A, Magos A, Emburey G, Versi E (1985) Long-term effects of the menopause and sex hormones on skin thickness. Br J Obstet Gynaecol 92: 256-259.
[46]
Frantz C, Stewart KM, Weaver VM (2010) The extracellular matrix at a glance. J Cell Sci 123: 4195-4200.
ADDRESS
Science Publishing Group
548 FASHION AVENUE
NEW YORK, NY 10018
U.S.A.
Tel: (001)347-688-8931