Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters
American Journal of Life Sciences
Volume 5, Issue 3, June 2017, Pages: 75-85
Received: Mar. 27, 2017; Accepted: Apr. 7, 2017; Published: May 8, 2017
Views 1025      Downloads 65
Aksana Hancharuk, Trivedi Global, Inc., Henderson, NV, USA
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, NV, USA
Alice Branton, Trivedi Global, Inc., Henderson, NV, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, NV, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, NV, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Article Tools
Follow on us
The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.
Consciousness Energy Healing Treatment, B16-F10, Extracellular Matrix, HaCaT, HFF-1, Hyaluronic Acid, Scratch Assay, Tetrahydrocurcumin
To cite this article
Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana, Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters, American Journal of Life Sciences. Vol. 5, No. 3, 2017, pp. 75-85. doi: 10.11648/j.ajls.20170503.11
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Helmstädter A, Staiger C (2014) Traditional use of medicinal agents: a valid source of evidence. Drug Discov Today 19: 4-7.
Fabricant DS, Farnsworth NR (2001) The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 109: 69-75.
Goyal RK (2005) Investigation of Cellular and Molecular Mechanisms for Anti-diabetic Drugs with Special Reference to Unani and Ayurvedic Herbal Medicines. In: Traditional System of Medicine, Abdin, M. Z. and Y. P. Abrol (Eds.). Narosa Publishing House, New Delhi.
WHO (1993) Research Guideline for Evaluating the Safety and Efficacy of Herbal Medicines. World Health Organization, Manila, Philippines.
Gao XH, Zhang L, Wei H, Chen HD (2008) Efficacy and safety of innovative cosmeceuticals. Clin Dermatol 26: 367-74.
Chanchal D, Swarnlata S (2008) Novel approaches in herbal cosmetics. J Cosmet Dermatol 7: 89-95.
Park K (2015) Role of micronutrients in skin health and function. Biomol Ther (Seoul) 23: 207-217.
Brinkhaus B, Lindner M, Schuppan D, Hahn EG (2000) Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica. Phytomedicine 75: 427-448.
Bhaskar Rao A, Prasad E, Deepthi SS, Haritha V, Ramakrishna S, Madhusudan K, Surekha MV, Venkata Rao YSR (2015) Wound healing: A new perspective on glucosylated tetrahydrocurcumin. Drug Des Devel Ther 0: 3579-3588.
Naito M, Wu X, Normura H, Kodama M, Kato Y, Osaswa T (2002) The protective effect of tetrahydrocurcumin on oxidative stress in cholesterol-fed rabbits. J Atheroscler Thromb 9: 243-250.
Aggarwal BB, Deb L, Prasad S (2015) Curcumin differs from tetrahydrocurcumin for molecular targets, signaling pathways and cellular responses. Molecules 20: 185-205.
Boyera N, Galey I, Bernard BA (1998) Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts. Int J Cosmet Sci 20: 151-158.
Movaffaghi Z, Farsi M (2009) Biofield therapies: Biophysical basis and biological regulations. Complement Ther Clin Pract 15: 35-37, 31.
Shenefelt PD (2014) Energy medicine in dermatology. In: Norman RA, Shenefelt PD, Rupani RN, editors. Integrative Dermatology. Oxford University Press, New York, NY, USA.
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 203-206.
Mahendra KT, Shrikant P, Harish S, Mayank G, Jana S (2015) An effect of biofield treatment on Multidrug-resistant Burkholderia cepacia: A multihost pathogen. J Trop Dis 3: 167.
Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Antimicrobial susceptibility pattern, biochemical characteristics and biotyping of Salmonella paratyphi A: An impact of biofield treatment. Clin Microbiol 4: 215.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after biofield treatment. Ind Eng Manage 4: 161.
Trivedi MK, Patil S, Tallapragada RM (2013) Effect of biofield treatment on the physical and thermal characteristics of silicon, tin and lead powders. J Material Sci Eng 2: 125.
Trivedi MK, Patil S, Tallapragada RM (2013) Effect of biofield treatment on the physical and thermal characteristics of vanadium pentoxide powder. J Material Sci Eng S11: 001.
Trivedi MK, Patil S, Tallapragada RM (2015) Effect of biofield treatment on the physical and thermal characteristics of aluminium powders. Ind Eng Manage 4: 151.
Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.
Altekar N, Nayak G (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
Shinde V, Sances F, Patil S, Spence A (2012) Impact of biofield treatment on growth and yield of lettuce and tomato. Aust J Basic Appl Sci 6: 100-105.
Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.
Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 8: 138-143.
Plumb JA (2004) Cell sensitivity assays: the MTT assay. Methods Mol Med 88: 165-69.
Hahn MS, Kobler JB, Starcher BC, Zeitels SM, Langer R (2006) Quantitative and comparative studies of the vocal fold extracellular matrix. I: Elastic fibers and hyaluronic acid. Ann Otol Rhinol Laryngol 115: 156-64.
Zhang L, Yoshida T, Kuroiwa Y (1992) Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin. Life Sci 51: 17-24.
Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I (2009) Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol 126: 463-467.
Kadler KE, Holmes DF, Trotter JA, Chapman JA (1996) Collagen fibril formation. Biochemical Journal 316: 1-11.
Shoulders MD, Raines RT (2009) Collagen structure and stability. Annual review of biochemistry 78: 929-958.
Frantz C, Stewart KM, Weaver VM (2010) The extracellular matrix at a glance. J Cell Sci 123: 4195-4200.
Weindl G, Schaller M, Schäfer-Korting M, Korting HC (2004) Hyaluronic acid in the treatment and prevention of skin diseases: molecular biological, pharmaceutical and clinical aspects. Skin Pharmacol Physiol 17: 207-213.
Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Brzezińska M (2013) Centella asiatica in cosmetology. Postepy Dermatol Alergol 30: 46-49.
Prasad S, Tyagi AK, Aggarwal BB (2014) Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. Cancer Res Treat 46: 2-18.
Busca R, Ballotti R (2000) Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res 13: 60-69.
Ho JN, Lee YH, Lee YD, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY (2005) Inhibitory effect of Aucubin isolated from Eucommia ulmoides against UVB induced matrix metalloproteinase-1 production in human skin fibroblasts. Biosci Biotechnol Biochem 69: 2227-2231.
Sumiyoshi M, Kimura Y (2009) Effects of a turmeric extract (Curcuma longa) on chronic ultraviolet B irradiation-induced skin damage in melanin-possessing hairless mice. Phytomedicine 16: 1137-1143.
Liang CC, Park AY, Guan JL (2007) In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2: 329-333.
Science Publishing Group
NEW YORK, NY 10018
Tel: (001)347-688-8931