Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat
American Journal of Life Sciences
Volume 5, Issue 4, August 2017, Pages: 108-115
Received: Jul. 8, 2017; Accepted: Jul. 17, 2017; Published: Aug. 11, 2017
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Abeer Saeed Alahmari, Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Biology Department, College of Science, King Khalid University, Abha, Saudi Arabia
Khadiga Gamal Eldeen Adham, Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Zoology Department, College of Science, Alexandria University, Alexandria, Egypt
Ahmad Rashed Alhimaidi, Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia
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Arsenic is a significant environmental public health concern. The aim of the present study is to investigate the possible immunotoxic and genotoxic roles of arsenic and the ameliorative effects of quercetin and probiotics as natural antioxidants. This study was performed on male adult Wistar rats divided into six groups: control, NaAsO2-treated, quercetin-treated, probiotic–treated, NaAsO2 and quercetin-treated, and NaAsO2 and probiotics-treated. Blood samples collected from all animals were prepared for some oxidative, immunological and genetic aspects. Administration of arsenic decreased body and spleen weight, reduced serum antioxidant defense parameters and DNA content, increased liver, kidney, and brain weights, plasma malondialdehyde (MDA), myeloperoxidase (MPO), and serum cytokines (IL-1β, IL-6, TNF-α and IFN-γ). Adding quercetin to arsenic was effective in restoring the altered values of cytokines (IL-1β and IL-6), MPO, MDA, catalase (CAT) and reduced glutathione (GSH) induced by arsenic, whereas the presence of probiotics was effective in reducing genotoxicity and improving the changes of cytokines (TNF-α and IFN-γ) and superoxide dismutase (SOD). Quercetin and probiotics are excellent antioxidant therapies, through their ability to suppress reactive oxygen species ROS production, which may contribute to arsenic toxicity.
Arsenic, Quercetin, Probiotics, Oxidative Stress, Lipid Peroxidation, Inflammation
To cite this article
Abeer Saeed Alahmari, Khadiga Gamal Eldeen Adham, Ahmad Rashed Alhimaidi, Immunotoxic and Genotoxic Effects of Arsenic and Ameliorative Potential of Quercetin and Probiotics in Wistar Rat, American Journal of Life Sciences. Vol. 5, No. 4, 2017, pp. 108-115. doi: 10.11648/j.ajls.20170504.13
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