Role of Explants and NAA on Callus Induction of Potato (Solanum tuberosum)
American Journal of Life Sciences
Volume 5, Issue 5, October 2017, Pages: 140-144
Received: Apr. 27, 2017; Accepted: Jul. 31, 2017; Published: Oct. 2, 2017
Views 480      Downloads 41
Md. Abu Kawochar, Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
Nizam Uddin Ahmed, Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
Md. Iqbal Hossain, Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
Jannatul Ferdous, Biotechnology Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh
Article Tools
Follow on us
An experiment was conducted to study the role of NAA and different explants on callus induction of potato varieties. There were three factors such as variety (Diamant, Heera and Cardinal), explants (leaves and internodes) and NAA levels (0, 2, 3, 4, 5 mg L-1). The experiment was laid out in complete randomized design with three replications. Minimum 6-8 days was required for callus initiation of internode of Cardinal in 3 mg L-1 NAA while the maximum 22-25 days was required for internode of Cardinal in 5 mg L-1 NAA. Both the leaf and internode of Heera produced 100% callus while Cardinal produced minimum callus (37.7 and 20.8% for leaf and internode, respectively) in 5 mg L-1 NAA. Neither leaf nor internode produced callus without NAA. Internode of Diamant, Heera and Cardinal produced 100% compact calli in 4, 3 and 5 mg L-1 NAA respectively. Leaf of Diamant without NAA produced the highest weight (0.667 g) of callus after one month. After two months, internode of Cardinal showed the highest weight (0.205 g) of calli in 3 mg L-1 NAA. Therefore, the present protocol has the potential for the rapid multiplication of true-to-type clones without changing the genetic fidelity.
Potato, Callus Induction, NAA, Explant
To cite this article
Md. Abu Kawochar, Nizam Uddin Ahmed, Md. Iqbal Hossain, Jannatul Ferdous, Role of Explants and NAA on Callus Induction of Potato (Solanum tuberosum), American Journal of Life Sciences. Vol. 5, No. 5, 2017, pp. 140-144. doi: 10.11648/j.ajls.20170505.14
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
FAO (Food and Agriculture Organization) (2014). Rome, Italy ( faostat/en/#data/QC) (Explore date: 23-07-2017).
BBS (Bangladesh Bureau of Statistics) (2016). The Year Book of Agricultural Statistics of Bangladesh, Dhaka. Statistics Division, Ministry of Planning, Govt. Peoples Republic of Bangladesh.
Rashid, B., Tariq, M., Khalid, A., Shams, F., Ali Q., Ashraf, F., Ghaffar, I., Khan, M. I., Rehman, R. and Husnain, T. (2017). Crop improvement: new approaches and modern techniques, Plant Gene and Trait, 8(3), 18-30.
Akter, N. (2001). Effects of different explants and concentrations of NAA on callus induction and regeneration of brinjal cv. Uttara. MSc Thesis. Bangladesh Agricultural University.
Hamdi, B., Khawla, A. and Messaoud, B. (2017). The effect of growth regulators and explants on callus induction in four cultivars of potato (Solanum tuberosum L.). Journal of Bioressources Valorization, 2(1), 34-41.
Dobranszki, J., Takacs, H. A., Magyar, T. K. and Ferenczy, A. (1999). Effect of medium on the callus forming capacity of different potato genotypes. Acta Agronomica Hungarica, 47(1), 59-61.
Hansen, J., Nielsen, B. and Nielsen, S. Vs. (1999). In vitro shoot regeneration of Solanum tuberosum cultivars: interactions of media composition and leaf, leaflet and explant position. Journal of the National Science Foundation of Sri Lanka, 27(1), 17-28.
Kaviani, B., Hesar, A. A., Tarang, A., Zanjani, S. B., Hashemabadi, D. and Ansari, M. H. (2013). Effect of kinetin (Kn) and naphthalene acetic acid (NAA) on the micropropagation of Matthiola incana using shoot tips, and callus induction and root formation on the leaf explants. African Journal of Agricultural Research, 8(30), 4134-4139.
Kumlay, A. M. and Ercisli, S. (2015). Callus induction, shoot proliferation and root regeneration of potato (Solanum tuberosum L.) stem node and leaf explants under long-day conditions. Biotechnology & Biotechnological Equipment, 29(6), 1075-1084.
Fomenko, T. I., Reshetnikov, V. N., Malyush, M. K., Kondratskaya, I. P. and Chumakova, I. M. (1998). Conditions for development of callus tissues of potato in vitro. Vestsi Akademii Navuk Belarusi. Seriya Biyalagichnykh Navuk, 4, 97-105.
Rabbani, A., Beenish, A., Abbasi, N. A., Mussarat, B. and Azra, Q. (2001). Effect of growth regulators on in vitro multiplication of potato. International Journal of Agriculture & Biology, 3(2), 181-182.
Murashige, T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3): 473-497.
Steel, R. G. D. and Torrie, J. H. (1960). Principles and procedures of statistics. New York, USA: McGraw Hall Book Publication. pp. 377-398.
Tikk, E. I. and Kollist, Yu E. (1992). Optimizing the conditions for induction of callus cultures in different varieties of potato. Sel' skokhozyaistvennaya Biologiya, 5: 37-40.
Roy AK and Rabbani MG. (2004). In vitro callus initiation and regeneration of four potato varieties of Bangladesh. 5th International Plant Tissue Culture and Biotechnology Conference. Dhaka, Bangladesh, 4-6 December 2004.
Fiegert, A. K., Mix-Wagner, G. and Vorlop, K. D. (2000). Regeneration of Solanum tuberosum L. cv. Tomensa: Induction of somatic embryogenesis in liquid culture for the production of “artificial seed”. Landbauforschung Völkenrode, 50(3/4), 199-202.
Kollist, Yu. and Tikk, E. (1994). Relationship of callus regeneration in potato to the conditions of induction. Eesti Teaduste Akadeemia Toimetised Bioloogia, 43(1): 12-17.
Science Publishing Group
NEW YORK, NY 10018
Tel: (001)347-688-8931