American Journal of Life Sciences
Volume 5, Issue 6, December 2017, Pages: 150-159
Received: Oct. 16, 2017;
Accepted: Oct. 30, 2017;
Published: Dec. 11, 2017
Views 3190 Downloads 136
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Aileen Carol Lee, Trivedi Global, Inc., Henderson, Nevada, USA
Aksana Hancharuk, Trivedi Global, Inc., Henderson, Nevada, USA
Carola Marina Sand, Trivedi Global, Inc., Henderson, Nevada, USA
Debra Jane Schnitzer, Trivedi Global, Inc., Henderson, Nevada, USA
Rudina Thanasi, Trivedi Global, Inc., Henderson, Nevada, USA
Eileen Mary Meagher, Trivedi Global, Inc., Henderson, Nevada, USA
Faith Ann Pyka, Trivedi Global, Inc., Henderson, Nevada, USA
Gary Richard Gerber, Trivedi Global, Inc., Henderson, Nevada, USA
Johanna Catharina Stromsnas, Trivedi Global, Inc., Henderson, Nevada, USA
Judith Marian Shapiro, Trivedi Global, Inc., Henderson, Nevada, USA
Laura Nelson Streicher, Trivedi Global, Inc., Henderson, Nevada, USA
Lorraine Marie Hachfeld, Trivedi Global, Inc., Henderson, Nevada, USA
Matthew Charles Hornung, Trivedi Global, Inc., Henderson, Nevada, USA
Patricia M. Rowe, Trivedi Global, Inc., Henderson, Nevada, USA
Sally Jean Henderson, Trivedi Global, Inc., Henderson, Nevada, USA
Sheila Maureen Benson, Trivedi Global, Inc., Henderson, Nevada, USA
Shirley Theresa Holmlund, Trivedi Global, Inc., Henderson, Nevada, USA
Stephen Phillip Salters, Trivedi Global, Inc., Henderson, Nevada, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
The aim of the present study was to evaluate the immunomodulatory potential of Biofield Energy Treatment (The Trivedi Effect®) on a new proprietary compound composed of herbs and minerals (herbomineral formulation). The test formulation contains the mixture of the herbal root extract ashwagandha and the minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated formulation. The immunomodulatory parameters viz. humoral immune response (IgG and IgM), cellular immune response (CD4+/CD8+), hematological parameters, lipid profile, hepatic enzymes, and sex hormones (progesterone and estrogen) in female Sprague Dawley rats were analyzed. The results of humoral immune response data showed decreased levels of IgG and IgM by 80.54% and 78.94% respectively, in the Biofield Energy Treated test formulation group (G3) compared with the disease control group (G2). Estimation of cellular immune response data revealed a significant (p≤0.01) increase in the ratio of CD4+/CD8+ by 161.71% in the G3 group, while it was increased by 100.50% in the untreated test formulation group (G4) compared with the G2 group. Thus, the humoral and cellular immune responses were significantly altered in the G3 as compared with the G4 group. The TLC and neutrophils counts were significantly increased by 11.34% and 1.34% respectively, in the G3 compared with the G2 group. Lipid profile data suggested that the Biofield Energy Treated test formulation showed improvement in the tested parameters compared with the untreated test formulation group. However, significant improvement was found in the tested hepatic biomarkers i.e. decreased levels of the SGOT, SGPT, and CK-MB by 14.28%, 8.54%, and 20.79% in the G3 group compared with the G2 group. The levels of total protein, alkaline phosphatase, albumin, and globulin were increased by 11.93%, 1.24%, 6.10%, and 20.74% respectively, in the G3 compared with the G2 group. Consequently, the levels of progesterone and estrogen were significantly increased by 199.86% and 50.19%, respectively in G3 with respect to the G2 group. In conclusion, overall data suggest that The Trivedi Effect®-Biofield Energy Healing Treatment on the herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management, and anti-aging by improving overall health.
Mahendra Kumar Trivedi,
Aileen Carol Lee,
Carola Marina Sand,
Debra Jane Schnitzer,
Eileen Mary Meagher,
Faith Ann Pyka,
Gary Richard Gerber,
Johanna Catharina Stromsnas,
Judith Marian Shapiro,
Laura Nelson Streicher,
Lorraine Marie Hachfeld,
Matthew Charles Hornung,
Patricia M. Rowe,
Sally Jean Henderson,
Sheila Maureen Benson,
Shirley Theresa Holmlund,
Stephen Phillip Salters,
Impact of Biofield Energy Treated (The Trivedi Effect®) Herbomineral Formulation on the Immune Biomarkers and Blood Related Parameters of Female Sprague Dawley Rats, American Journal of Life Sciences.
Vol. 5, No. 6,
2017, pp. 150-159.
Thomson GE (2007) The Health Benefits of Traditional Chinese Plant Medicines: Weighing the Scientific Evidence: A Report for the Rural Industries Research and Development Corporation, RIRDC, Barton, Australia.
Rishton GM (2008) Natural products as a robust source of new drugs and drug leads: Past successes and present day issues. Am J Cardiol 101: 43D-49 D.
Bhaskaran-Nair Harikumar K, Hardman R, Aranjani JM, Vimala Raveendran V, Thejass P (2014) Immunomodulatory activity of complementary and alternative medicines. Evid Based Complement Alternat Med 2014: 765107.
Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, Van Rompay M, Kessler RC (1998) Trends in alternative medicine use in the United States, 1990-1997: results of a follow-up national survey. JAMA 280: 1569-1575.
Janeway CA Jr (2001) How the immune system protects the host from infection. Microbes Infect 3: 1167-1171.
Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on the immunomodulatory effects of ashwagandha. J Ethnopharmacol 50: 69-76.
Lukác N, Massányi P (2007) Effects of trace elements on the immune system. Epidemiol Mikrobiol Imunol 56: 3-9.
Galland L (1998) Magnesium and immune function: An overview. Magnesium 7: 290‐299.
Wintergerst ES, Maggini S, Hornig DH (2007) Contribution of selected vitamins and trace elements to immune function. Ann Nutr Metab 51: 301‐323.
Girdhari L, Rana A (2007) Withania somnifera (Ashwagandha): A review. Pharmacogn Rev 1: 129-136.
Singh N, Bhalla M, de Jager P, Gilca M (2011) An overview on ashwagandha: A Rasayana (Rejuvenator) of Ayurveda. Afr J Tradit Complement Altern Med 8: 208-213.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
Rubik B, Muehsam D, Hammerschlag R, Jain S (2015) Biofield Science and Healing: History, Terminology, and Concepts. Glob Adv Health Med 4: 8-14.
Debas HT, Laxminarayan R, Straus SE. Complementary and Alternative Medicine. In: Jamison DT, Breman JG, Measham AR., editors. Disease Control Priorities in Developing Countries. 2nd edition. Washington (DC): The International Bank for Reconstruction and Development / The World Bank; 2006. Chapter 69. Available from: https://www.ncbi.nlm.nih.gov/books/NBK11796/ Co-published by Oxford University Press, New York.
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antimicrobial sensitivity, biochemical characteristics and biotyping of Staphylococcus saprophyticus: An impact of biofield energy treatment. J Women’s Health Care 4: 271.
Trivedi MK, Branton A, Trivedi D, Shettigar H, Nayak G, Mondal SC, Jana S (2015) Phenotyping and genotyping characterization of Proteus vulgaris after biofield treatment. International Journal of Genetics and Genomics 3: 66-73.
Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Antibiogram pattern of Shigella flexneri: Effect of biofield treatment. Air Water Borne Diseases 3: 122.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Characterization of phenotype and Genotype of Biofield Treated Enterobacter aerogenes. Transl Med 5: 155.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Bacterial identification using 16S rDNA gene sequencing and antibiogram analysis on biofield treated Pseudomonas fluorescens. Clin Med Biochemistry Open Access 1: 101.
Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Mass spectrometric analysis of isotopic abundance ratio in biofield energy treated thymol. Frontiers in Applied Chemistry 1: 1-8.
Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Determination of isotopic abundance of 13C/12C or 2H/1H and 18O/16O in biofield energy treated 1-chloro-3-nitrobenzene (3-CNB) using gas chromatography-mass spectrometry. Science Journal of Analytical Chemistry 4: 42-51.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth regulator, immunity and DNA fingerprinting of biofield energy treated mustard seeds (Brassica juncea). Agriculture, Forestry and Fisheries 4: 269-274.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3: 245-250.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of biofield energy treatment on physical and thermal characteristics of selenium powder. Journal of Food and Nutrition Sciences 3: 223-228.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3: 129.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Physical, atomic and thermal properties of biofield treated lithium powder. J Adv Chem Eng 5: 136.
Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa k, Jana s (2015) Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium, Advances in Biochemistry 3: 77-85.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, Mishra R (2015) Evaluation of the Impact of Biofield Treatment on Physical and Thermal Properties of Casein Enzyme Hydrolysate and Casein Yeast Peptone. Clin Pharmacol Biopharm 4: 138.
Trivedi MK, Branton A, Trivedi D, Nayak G, Plikerd WD, Surguy PL, Kock RJ, Piedad RB, Callas RP, Ansari SA, Barrett SL, Friedman S, Christie SL, Liu SC, Starling SE, Jones S, Allen SM, Wasmus SK, Benczik TA, Slade TC, Orban T, Vannes VL, Schlosser VM, Albino YSY, Panda P, Sethi KK, Jana S (2017) A Systematic study of the biofield energy healing treatment on physicochemical, thermal, structural, and behavioral properties of magnesium gluconate. International Journal of Bioorganic Chemistry 2: 135-145.
Trivedi MK, Branton A, Trivedi D, Nayak G, Plikerd WD, Surguy PL, Kock RJ, Piedad RB, Callas RP, Ansari SA, Barrett SL, Friedman S, Christie SL, Liu SC, Starling SE, Jones S, Allen SM, Wasmus SK, Benczik TA, Slade TC, Orban T, Vannes VL, Schlosser VM, Albino YSY, Panda P, Sethi KK, Jana S (2017) Chromatographic and spectroscopic characterization of the consciousness energy healing treated Withania Somnifera (ashwagandha) root extract. European Journal of Biophysics 5: 38-47.
Ladics GS (2007) Primary immune response to sheep red blood cells (SRBC) as the conventional T-cell dependent antibody response (TDAR) test. J Immunotoxicol 4: 149-152.
Yamada K, Hung P, Park TK, Park PJ, Lim BO (2011) A comparison of the immunostimulatory effects of the medicinal herbs Echinacea, Ashwagandha and Brahmi. J Ethnopharmacol 137: 231-235.
Malik F, Singh J, Khajuria A, Suri KA, Satti NK, Singh S, Kaul MK, Kumar A, Bhatia A, Qazi GN (2007) A standardized root extract of Withania somnifera and its major constituent withanolide-A elicit humoral and cell-mediated immune responses by up regulation of Th1-dominant polarization in BALB/c mice. Life Sci 80: 1525-1538.
Spallholz JE, Stewart JR (1989) Advances in the role of minerals in immunobiology. Biol Trace Elem Res 19: 129-151.
Uppal SS, Verma S, Dhot PS (2003) Normal values of CD4 and CD8 lymphocyte subsets in healthy Indian adults and the effects of sex, age, ethnicity, and smoking. Cytometry B Clin Cytom 52: 32-36.
Mikolai J, Erlandsen A, Murison A, Brown KA, Gregory WL, Raman-Caplan P, Zwickey HL (2008) In vivo effects of ashwagandha (Withania somnifera) extract on the activation of lymphocytes. J Altern Complement Med 15: 423-430.
Madhuri S, Pandey G, Khanna A, Shrivastav AB (2012) Effect of some herbal drugs on haematological profiles of rats. IRJP 3: 158-160.
Oyedemi S, Adewusi E, Aiyegoro O, Akinpelu D (2011) Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia Africana (Smith) on streptozotocin-induced diabetic Wistar rats. Asian Pac J Trop Biomed 1: 353-358.
Durrani FR, Abidullah NC, Durrani Z, Akhtar S (2008) Hematological, biochemical, immunomodulatory and growth promoting effect of feed added wild mint (Mentha longifolia) in broiler chicks. Sarhad J Agric 24: 661-664.
El Hendy HA, Yousef MI, Abo El-Naga NI (2001) Effect of dietary zinc deficiency on hematological and biochemical parameters and concentrations of zinc, copper, and iron in growing rats. Toxicology 167: 163-170.
Alimohamady R, Aliarabi H, Bahari A, Dezfoulian AH (2013) Influence of different amounts and sources of selenium supplementation on performance, some blood parameters, and nutrient digestibility in lambs. Biol Trace Elem Res 154: 45-54.
Cinar V, Nizamlioglu M, Mogulkoc R, Baltaci AK (2007) Effects of magnesium supplementation on blood parameters of athletes at rest and after exercise. Biol Trace Elem Res 115: 205-212.
Andallu B, Radhika B (2000) Hypoglycemic, diuretic and hypocholesterolemic effect of winter cherry (Withania somnifera, Dunal) root. Indian J Exp Biol 38: 607-609.
Bunglavan SJ, Garg AK, Dass RS, Shrivastava S (2014) Effect of supplementation of different levels of selenium as nanoparticles/sodium selenite on blood biochemical profile and humoral immunity in male Wistar rats. Vet World 7: 1075-1081.
Fox C, Ramsoomair D, Carter C (2001) Magnesium: Its proven and potential clinical significance. South Med J 94: 1195-1201.
Payahoo L, Ostadrahimi A, Mobasseri M, Bishak YK, Farrin N, Jafarabadi MA, Mahluji S (2013) Effects of zinc supplementation on the anthropometric measurements, lipid profiles and fasting blood glucose in the healthy obese adults. Adv Pharm Bull 3: 161-165.
Torres-Duran PV, Ferreira-Hermosillo A, Juarez-Oropeza MA, Elias-Viñas D, Verdugo-Diaz L (2007) Effects of whole body exposure to extremely low frequency electromagnetic fields (ELF-EMF) on serum and liver lipid levels, in the rat. Lipids Health Dis 6: 31.
Giannini EG, Testa R, Savarino V (2005) Liver enzyme alteration: A guide for clinicians. CMAJ 172: 367-379.
Sidhu P, Garg ML, Dhawan DK (2005) Protective effects of zinc on oxidative stress enzymes in liver of protein-deficient rats. Drug Chem Toxicol 28: 211-230.
El-Boshy ME, Risha EF, Abdelhamid FM, Mubarak MS, Hadda TB (2015) Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats. J Trace Elem Med Biol 29: 104-110.
Karandish M, Tamimi M, Shayesteh AA, Haghighizadeh MH, Jalali MT (2013) The effect of magnesium supplementation and weight loss on liver enzymes in patients with nonalcoholic fatty liver disease. J Res Med Sci 18: 573-579.
Sabiba EP, Rasool M, Vedi M, Navaneethan D, Ravichander M, Parthasarathy P, Thella SR (2013) Hepatoprotective and antioxidant potential of Withania somnifera against paracetamol-induced liver damage in rats. Int J Pharm Pharm Sci 5: 648-651.
Sunar F, Baltaci AK, Ergene N, Mogulkoc R (2009) Zinc deficiency and supplementation in ovariectomized rats: Their effect on serum estrogen and progesterone levels and their relation to calcium and phosphorus. Pak J Pharm Sci 22: 150-154.
Zagrodzki P, Ratajczak R (2008) Selenium status, sex hormones, and thyroid function in young women. J Trace Elem Med Biol 22: 296-304.
Belal NM, El-Metwally EM, Salem IS (2012) Effect of dietary intake ashwagandha roots powder on the levels of sex hormones in the diabetic and non-diabetic male rats. World J Dairy & Food Sci 7: 160-166.