A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure
American Journal of Life Sciences
Volume 6, Issue 1, February 2018, Pages: 7-12
Received: Mar. 7, 2018;
Published: Mar. 9, 2018
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Jiayi Lu, Department of Biological Sciences, Denison University, Granville, USA
Jorge De La Torre, Department of Biological Sciences, Denison University, Granville, USA
Carson McCann, Department of Biological Sciences, Denison University, Granville, USA
Maureen Madar, Department of Biological Sciences, Denison University, Granville, USA
Qiongqiong Zhou, Department of Biological Sciences, Denison University, Granville, USA
HeLa cells were derived from human cervical cancer, which has been widely used as research model to study mammalian cell functions. In this work, nocodazole, a microtubule destabilizer, was used to treat HeLa cells with different concentrations. The results showed that nocodazole was able to inhibit HeLa cell’s growth rate significantly at the concentration of 100 nM. It suggested that nocodazole may inhibit cell growth through an alternative impacting effect other than destabilizing microtubules, since the effect of nocodazole destabilizing microtubule is usually not seen at micromolar range. However, at nanomolar concentration, nocodazole was not able to induce any changes in F-actin structure. Whereas, at concentration of 1 mM, nocodazole induced significant alterations of F-actin structure in HeLa cells. It indicated the strong relationship between microtubule and actin dynamics. Our work suggested that a molecule may exhibit different impacting mechanisms at different concentrations. Since nocodazole has been used as a chemotherapy reagent in cancer treatment, it will be beneficial to re-evaluate the effective concentration in terms of cancer treatment.
Jorge De La Torre,
A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure, American Journal of Life Sciences.
Vol. 6, No. 1,
2018, pp. 7-12.
Masters JR. Nat Rev Cancer. 2002 Apr;2(4):315-9. Review.
Beskow LM. Annu Rev Genomics Hum Genet. 2016 Aug 31;17:395-417. doi: 10.1146/annurev-genom-083115-022536. Epub 2016 Mar 3. Review.
AccessScience Editors. (2014). Importance of HeLa cells. In AccessScience. McGraw-Hill Education.
Blajeski, A. L., Phan, V. A., Kottke, T. J., & Kaufmann, S. H. (2002). G1 and G2cell-cycle arrest following microtubule depolymerization in human breast cancer cells. The Journal of Clinical Investigation, 110(1), 91–99.
Jordan, M. A., Thrower, D., & Wilson, L. (1992). Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis. J cell Sci, 102(3), 401-416.
Vasquez, R. J., Howell, B., Yvon, A. M., Wadsworth, P., & Cassimeris, L. (1997). Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Molecular biology of the cell, 8(6), 973-985.
Ngo T, Miao X, Robinson DN, Zhou QQ. Acta Pharmacol Sin. 2016 Nov;37(11):1449-1457. doi: 10.1038/aps.2016.57. Epub 2016 Aug 29.
Zhou Q, Kee YS, Poirier CC, Jelinek C, Osborne J, Divi S, Surcel A, Will ME, Eggert US, Müller-Taubenberger A, Iglesias PA, Cotter RJ, Robinson DN. Curr Biol. 2010 Nov 9;20(21):1881-9. doi: 10.1016/j.cub.2010.09.048. Epub 2010 Oct 14.
April L. Blajeski, Vy A. Phan, Timothy J. Kottke, Scott H. Kaufmann, J Clin Invest. 2002 Jul 1; 110(1): 91–99. doi: 10.1172/JCI13275.
Vasquez RJ, Howell B, Yvon AM, Wadsworth P, Cassimeris L., Mol Biol Cell. 1997 Jun;8(6):973-85.
Poulter NS, Thomas SG. Int J Biochem Cell Biol. 2015 Sep;66:69-74. doi: 10.1016/j.biocel.2015.07.008. Epub 2015 Jul 23. Review.
Akhshi TK, Wernike D, Piekny A. Cytoskeleton (Hoboken). 2014 Jan;71(1):1-23. doi: 10.1002/cm.21150. Epub 2013 Oct 29. Review.
Cabrales Fontela Y, Kadavath H, Biernat J, Riedel D, Mandelkow E, Zweckstetter M. Nat Commun. 2017 Dec 7;8(1):1981. doi: 10.1038/s41467-017-02230-8.
HIRAMOTO Y. J Cell Biol. 1965 Apr;25:SUPPL:161-7.
Sider, J. R., Mandato, C. A., Weber, K. L., Zandy, A. J., Beach, D., Finst, R. J.,. Bement, W. M. (1999). Journal of Cell Science, 112(12), 1947.
Lutz, D. A., Hamaguchi, Y. & Inoue, S. (1988). Cell Motil. Cytoskeleton 11, 83–96.
Forscher, P., Bement, W. M., Waterman-Storer, C. M., Mandato, C. A., Schaefer, A. W., & Rodriguez, O. C. (2003). Nature Cell Biology, 5(7), 599-609.
Palazzo, A. F., Cook, T. A., Alberts, A. S. & Gundersen, G. G. (2001). Nature Cell Biol. 3, 723–729.
Peitzsch, C., Tyutyunnykova, A., Pantel, K., & Dubrovska, A. (2017, February). In Seminars in Cancer Biology. Academic Press.
Meck RA, Carsten AL, Kelsch JJ. Cancer Res. 1976 Jul; 36(7 PT 1):2317-20.