American Journal of Life Sciences

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Ameliorative Effect of Probiotic Strains, Lactobacillus Acidophilus and Lactobacillus Bulgaricus against Acetic Acid-Induced Inflammation in the Mouse Colon

Received: 13 January 2020    Accepted: 27 January 2020    Published: 23 November 2020
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Abstract

Probiotic bacteria with anti-inflammatory properties have the potential to be of therapeutic benefit in inflammatory bowel diseases. The present study was designed to evaluate the effect of feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus on acetic acid-induced inflammation in mouse colon. Inflammatory model that mimics various features of IBDs was induced by a single application of 100µl of 4.5% acetic acid in Swiss Albino mice. Mice were pretreated orally by 200µl yogurt containing both L. acidophilus and L. bulgaricus for 3 days before induction of inflammation and 200µl yogurt was given orally for a period of 7 days after acetic-acid induction. The body weight, food and water intakes, serum biomarkers, macroscopic and histopathological studies of colon tissues were performed to evaluate the anti-inflammatory effect. Combined administration of both strains prevented the damages of villous and crypts in colon epithelial cells and thus provides unique mucosal protective effects in experimental colitis. In conclusion, feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus prevented or ameliorated the inflammatory conditions that can be beneficial to prevent or lower risks of IBDs and its complications.

DOI 10.11648/j.ajls.20200806.11
Published in American Journal of Life Sciences (Volume 8, Issue 6, December 2020)
Page(s) 183-188
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Probiotics, Inflammatory Bowel Diseases, Acetic Acid-induced Inflammation

References
[1] Natividad J, Verdu E: Modulation of intestinal barrier by intestinal microbiota: pathological and therapeutic implications. Pharmacological Res 2013, 69 (1): 42-51.
[2] Silva HD, Millard P, Soper N, Kettlewell M, Mortensen N, Jewell D: Effects of the faecal stream and stasis on the ileal pouch mucosa. Gut 1991, 32 (10): 1166-1169.
[3] Clayburgh DR, Shen L, Turner JR: A porous defense: the leaky epithelial barrier in intestinal disease. Lab Invest 2004, 84: 282–291.
[4] Hollander D: The intestinal permeability barrier. A hypothesis as to its regulation and involvement in Crohn’s disease. Scand J Gastroenterol 1992, 27: 721–726.
[5] Famularo G, Moretti S, Marcellini S, De Simone C: Stimulation of immunity by probiotics. In: Fuller R, ed. Probiotics: therapeutic and other beneficial effects. London: Chapman and Hall, 1997, 13361.
[6] Collado MC, Isolauri E, Salminen S, Sanz Y: The Impact of Probiotic on Gut Health. Current Drug Metabolism 2009, 10: 68-78.
[7] Bermudez-Brito M, Plaza-Díaz J, Muñoz-Quezada S, Gómez-Llorente C, Gil A: Probiotic mechanisms of action. Ann Nutr Metab 2012, 61: 160-174.
[8] Plaza-Díaz J, Fernandez-Caballero JÁ, Chueca N, Garcia F, Gómez-Llorente C, Sáez-Lara MJ, Fontana L, Gil A: Pyrosequencing analysis reveals changes in intestinal microbiota of healthy adults who received a daily dose of immunomodulatory probiotic strains. Nutrients 2015, 7: 3999-4015.
[9] Plaza-Diaz J, Gomez-Llorente C, Abadia-Molina F, Saez-Lara MJ, Campaña-Martin L, Muñoz-Quezada S, Romero F, Gil A, Fontana L: Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on hepatic steatosis in Zucker rats. PLoS ONE 2014, 9.
[10] Fuller R: Probiotics in man and animals. J Appl Bacteriol 1989, 66: 365-378.
[11] Abdelouhab K, Rafa H, Toumi R, Bouaziz S, Medjeber O, Touil-Boukoffa C: Mucosal intestinal alteration in experimental colitis correlates with nitric oxide production by peritoneal macrophages: Effect of probiotics and prebiotics. Immunopharmacol Immunotoxicol 2012, 34: 590–597.
[12] Toumi R, Abdelouhab K, Rafa H, Soufli I, Raissi-Kerboua D, Djeraba Z, Touil-Boukoffa C: Beneficial role of the probiotic mixture Ultra biotique on maintaining the integrity of intestinal mucosal barrier in DSS-induced experimental colitis. Immunopharmacol Immunotoxicol 2013, 35: 403-409.
[13] Kumar A, Kumar D: Isolation and characterization of bacteria from dairy samples of Solan in Himachal Pradesh for identification of Lactobacillus spp. Int J Pharm Sci Rev Res 2014, 25 (2): 110-114.
[14] Neurath MF, Fuss I, Kelsall BL, Stüber E, Strober W: Antibodies to interleukin 12 abrogate established experimental colitis in mice. J Exp Med 1995, 182: 1281-1290.
[15] Rachmilewitz D, Karmeli F, Takabayashi K, Hayashi T, Leider-Trejo L, Lee J, Leoni LM, Raz E: Immunostimulatory DNA ameliorates experimental and spontaneous murine colitis. Gastroenterology 2002, 122: 1428-1441.
[16] Niu X, Fan T, Li W, Huang H, Zhang Y, Xing W: Protective effect of sanguinarine against acetic acid-induced ulcerative colitis in mice. Toxicol Appl Pharmacol 2013, 267: 256-265.
[17] Ramana MV, Mukharjee NSV, Ramesh A, Manohar M, Hindu N, Vanaja T, Pavani B, Santhosh N, Srikanth E, Venkatesh M et al: Effect of Sulfasalazine and Prednisolone against dextran induced ulcerative colitis in female balb/c mice. Journal of Pharmacy Research 2012, 5 (9): 4808-4811.
[18] Triantafillidis JK, Douvi G, Agrogiannis G, Patsouris E, Gikas A, Papalois AE: Effect of Mesalamine and Prednisolone on TNBS Experimental Colitis, following Various Doses of Orally Administered Iron. BioMed Research International 2014, 2014: 8.
[19] Eckburg PB, Bik EM, Bernstein CN: Microbiology: diversity of the human intestinal microbial flora. Science 2005, 308 (5728): 1635-1638.
[20] Sartor RB: Mechanisms of disease: pathogenesis of Crohn's disease and ulcerative colitis. Nature Clinical Practice Gastroenterology and Hepatology 2006, 3 (7): 390–407.
[21] Sohag MSU, Paul M, Al-Bari MAA, Wahed MII, Khan MRI: Potential antidiabetic activities of probiotic strains, L. acidophilus and L. bulgaricus against fructose-fed hyperglycemic rats. Food and Nutrition Sci 2019, 10, 1419-1432.
[22] Keshavarzian A, Sedghi S, Kanofsky J, List T, Robinson C, Ibrahim C, Winship D: Excessive production of reactive oxygen metabolites by inflamed colon: analysis by chemiluminescence probe. Gastroenterology 1992, 103: 177-185.
[23] Closa D, Folch-Puy E: Oxygen free radicals and the systemic inflammatory response. IUBMB Life 2004, 56: 185-191.
[24] Grisham MB, Granger DN: Neutrophil-mediated mucosal injury. Role of reactive oxygen metabolites. Dig Dis Sci 1988, 33 (3): 6S-15.
[25] Lin MY, Yen CL: Antioxidative ability of lactic acid bacteria. J Agric Food Chem 1999, 47: 1460-1466.
[26] Vastano V, Pagano A, Fusco A, Merola G, Sacco M, Donnarumma G: The Lactobacillus plantarum Eno A1 enolase is involved in immunostimulation of Caco-2 cells and in biofilm development. Adv Exp Med Biol 2016, 897: 33-44.
[27] Zyrek AA, Cichon C, Helms S, Enders C, Sonnenborn U, Schmidt MA: Molecular mechanisms underlying the probiotic effects of Escherichia coli Nissle 1917 involve ZO-2 and PKCzeta redistribution resulting in tight junction and epithelial barrier repair. Cell Microbiol 2007, 9: 804-816.
[28] Schlee M, Harder J, Koten B, Stange EF, Wehkamp J, Fellermann K: Probiotic lactobacilli and VSL#3 induce enterocyte β-defensin 2. Clin Exp Immunol 2008, 151: 528-535.
[29] Resta-Lenert S, Barrett KE: Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive Escherichia coli (EIEC). Gut 2003, 52: 988-997.
Author Information
  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh; Beximco Pharmaceuticals Ltd., Gazipur, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh; Department of Pharmacy, Comilla University, Comilla, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

Cite This Article
  • APA Style

    Md Shihab Uddin Sohag, Mst. Mahfuza Rahman, Mollika Paul, Md. Masud Rana, Ranjan Kumar Barman, et al. (2020). Ameliorative Effect of Probiotic Strains, Lactobacillus Acidophilus and Lactobacillus Bulgaricus against Acetic Acid-Induced Inflammation in the Mouse Colon. American Journal of Life Sciences, 8(6), 183-188. https://doi.org/10.11648/j.ajls.20200806.11

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    ACS Style

    Md Shihab Uddin Sohag; Mst. Mahfuza Rahman; Mollika Paul; Md. Masud Rana; Ranjan Kumar Barman, et al. Ameliorative Effect of Probiotic Strains, Lactobacillus Acidophilus and Lactobacillus Bulgaricus against Acetic Acid-Induced Inflammation in the Mouse Colon. Am. J. Life Sci. 2020, 8(6), 183-188. doi: 10.11648/j.ajls.20200806.11

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    AMA Style

    Md Shihab Uddin Sohag, Mst. Mahfuza Rahman, Mollika Paul, Md. Masud Rana, Ranjan Kumar Barman, et al. Ameliorative Effect of Probiotic Strains, Lactobacillus Acidophilus and Lactobacillus Bulgaricus against Acetic Acid-Induced Inflammation in the Mouse Colon. Am J Life Sci. 2020;8(6):183-188. doi: 10.11648/j.ajls.20200806.11

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  • @article{10.11648/j.ajls.20200806.11,
      author = {Md Shihab Uddin Sohag and Mst. Mahfuza Rahman and Mollika Paul and Md. Masud Rana and Ranjan Kumar Barman and Mir Imam Ibne Wahed and Md. Rafiqul Islam Khan},
      title = {Ameliorative Effect of Probiotic Strains, Lactobacillus Acidophilus and Lactobacillus Bulgaricus against Acetic Acid-Induced Inflammation in the Mouse Colon},
      journal = {American Journal of Life Sciences},
      volume = {8},
      number = {6},
      pages = {183-188},
      doi = {10.11648/j.ajls.20200806.11},
      url = {https://doi.org/10.11648/j.ajls.20200806.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20200806.11},
      abstract = {Probiotic bacteria with anti-inflammatory properties have the potential to be of therapeutic benefit in inflammatory bowel diseases. The present study was designed to evaluate the effect of feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus on acetic acid-induced inflammation in mouse colon. Inflammatory model that mimics various features of IBDs was induced by a single application of 100µl of 4.5% acetic acid in Swiss Albino mice. Mice were pretreated orally by 200µl yogurt containing both L. acidophilus and L. bulgaricus for 3 days before induction of inflammation and 200µl yogurt was given orally for a period of 7 days after acetic-acid induction. The body weight, food and water intakes, serum biomarkers, macroscopic and histopathological studies of colon tissues were performed to evaluate the anti-inflammatory effect. Combined administration of both strains prevented the damages of villous and crypts in colon epithelial cells and thus provides unique mucosal protective effects in experimental colitis. In conclusion, feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus prevented or ameliorated the inflammatory conditions that can be beneficial to prevent or lower risks of IBDs and its complications.},
     year = {2020}
    }
    

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    T1  - Ameliorative Effect of Probiotic Strains, Lactobacillus Acidophilus and Lactobacillus Bulgaricus against Acetic Acid-Induced Inflammation in the Mouse Colon
    AU  - Md Shihab Uddin Sohag
    AU  - Mst. Mahfuza Rahman
    AU  - Mollika Paul
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    AU  - Md. Rafiqul Islam Khan
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    DO  - 10.11648/j.ajls.20200806.11
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
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    PB  - Science Publishing Group
    SN  - 2328-5737
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    AB  - Probiotic bacteria with anti-inflammatory properties have the potential to be of therapeutic benefit in inflammatory bowel diseases. The present study was designed to evaluate the effect of feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus on acetic acid-induced inflammation in mouse colon. Inflammatory model that mimics various features of IBDs was induced by a single application of 100µl of 4.5% acetic acid in Swiss Albino mice. Mice were pretreated orally by 200µl yogurt containing both L. acidophilus and L. bulgaricus for 3 days before induction of inflammation and 200µl yogurt was given orally for a period of 7 days after acetic-acid induction. The body weight, food and water intakes, serum biomarkers, macroscopic and histopathological studies of colon tissues were performed to evaluate the anti-inflammatory effect. Combined administration of both strains prevented the damages of villous and crypts in colon epithelial cells and thus provides unique mucosal protective effects in experimental colitis. In conclusion, feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus prevented or ameliorated the inflammatory conditions that can be beneficial to prevent or lower risks of IBDs and its complications.
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