Variations in Biochemical Attributes of Cassia tora L. and C. auriculata L. under Temperature Stress
American Journal of Life Sciences
Volume 2, Issue 6-1, November 2014, Pages: 16-21
Received: Aug. 16, 2014;
Accepted: Sep. 3, 2014;
Published: Sep. 17, 2014
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Geetika Pant, Department of Biotechnology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Sibi G., Department of Biotechnology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Sangeetha Annie George, Department of Zoology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Shubha Bhadran, Department of Genetics, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Ugam Chauhan, Department of Biotechnology, A. P. S. University, Madhya Pradesh, INDIA
Plants continuously struggle for survival under various environmental abiotic stress conditions, specifically high temperature. Fourteen-day-old seedlings of Cassia tora and Cassia auriculata were subjected to differential temperature stress treatments at 30ºC, 37ºC, 42°C and 44°C for 16 h each. Various biochemical parameters viz reducing sugars, total protein, chlorophyll content and antioxidant enzyme system were assessed and found to be increased under high temperature stress. The amount of reducing sugars, total protein and chlorophyll were remarkably increased in both the Cassia species at 42ºC. The POX activity was more profound in C. tora (0.41 U/mg) than C. auriculata (0.24 U/mg) at 42°C. However, the activity of Catalase in both the species recorded a similar effect with a maximum value of 0.39 and 0.43 U/mg in C. tora and C. auriculata respectively. Similarly, SOD percentage inhibition activity increased significantly at 42°C for C. tora and C. auriculata showing a noticeable trend of inhibition of 85.23% and 86.89% respectively. Thus it can be concluded that various osmolytes and an efficient antioxidative system play a key role in generating tolerance against temperature stress and maintaining homeostasis to withstand the maximum range for survival at 42ºC in Cassia seedlings.
Sangeetha Annie George,
Variations in Biochemical Attributes of Cassia tora L. and C. auriculata L. under Temperature Stress, American Journal of Life Sciences. Special Issue:Recent Developments in Health Care through Plants and Microbes.
Vol. 2, No. 6-1,
2014, pp. 16-21.
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