Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot
American Journal of Life Sciences
Volume 2, Issue 6-2, December 2014, Pages: 11-18
Received: Aug. 20, 2014;
Accepted: Aug. 25, 2014;
Published: Sep. 5, 2014
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Barakat F. M., Plant Pathology Department, Faculty of Agriculture, Cairo University, Egypt
Abada K. A., Plant Pathology Department, Faculty of Agriculture, Cairo University, Egypt
Abou-Zeid N. M., Plant Pathology Research Institute, ARC. Giza, Egypt
El-Gammal Y. H. E., Plant Pathology Research Institute, ARC. Giza, Egypt
Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).
Barakat F. M.,
Abada K. A.,
Abou-Zeid N. M.,
El-Gammal Y. H. E.,
Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot, American Journal of Life Sciences. Special Issue: Role of Combination Between Bioagents and Solarization on Management of Crown-and Stem-Rot of Egyptian Clover.
Vol. 2, No. 6-2,
2014, pp. 11-18.
Abd EI-Moity TH, Abou-Zeid NM, Tawfik AE (1990). Correlation between microbial populations in phylloplane of faba bean varieties and their reaction to Botrytis fabae. Agric. Res. Rev. 68 (3): 433- 441.
Abd-El-Khair HR, Khalifa KM, Karima HE, Haggag (2010). Effect of Trichoderma spp. on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. J. American Sci., 6 (9):486-497.
Abou-Zeid NM, Hassanien AM (2000). Biological control of chocolate spot disease (Botrytis fabae Sard.) in faba bean in Egypt. Phytophathology, 90:1182.
Abou-Zied NM, Hassanein AM, EL-Behery MH (2003). Biological control of damping -off and root- rot diseases of faba bean caused by Rhizoctonia in faba bean, lentil and chickpea and chocolate spot disease in faba bean / ICARDA / NVR SRP. Cairo. Egypt. viil-66p. ISBN : 977-302-073-8pp-49-52.
Ajith PS, Lakshmidevi N (2010). Effect of volatile and non-volatile compounds from Trichoderma spp. against Colletotrichum capsici incitant of Anthracnose on Bell. Nature and Science., 8 (9):265-269.
Akitar CM (1982). Investigation of the biological control of Rhizoctonia solani and Fusarium oxyspomm f.sp. lentil root-rot and wilt disease in cotton and linseed crops, respectively. Final report of project No.PK-ARS 83, AAIMRIV A, Fasulabed.
Barnett HL, Hunter BB (1987). Illustrated Genera of Imperfect Fungi. (4th ed.). MacMillan Pub. Co., New York, U.S.A., 218 PP.
Bissett J. (1991). A revision of the genus Trichoderma.W: Infragenic classification. Can. J. Bot., 69:2357-2317.
Boubekeur SB, Mahiout D, Benzohra IE, Benkada MY (2012). Antagonism of three Trichoderma species against Botrytis fabae and B. cinerea, the causal agents of choco-late spot of faba bean (Vicia faba L.) in Algeria. World Appl. Sci. J., 17 (3): 278¬283.
Bouhassan A, Sadiki M, Tivoli B (2004). Evaluation of a collection of faba bean (Vicia fabae L.) genotypes originating from the Maghreb for resistance to chocolate spot (Botrytis fabae) by assessment in the field and laboratory. Euphytica 135:55-62.
Brewer MT, Larkin RP (2005). Efficacy of several potential biocontrol organisms against Rhizoctonia solani on potato. Crop Protection, 24: 939-950.
Brown W (1924). Two mycological methods: 11.A method of isolating single strains of fungi by cutting a hyphal tip. Ann. Bot., 38: 404.
Chet I, Inbar J, Hadar I (1997). Fungal antagonists and mycoparasites. In: The Mycota IV: Environmental and Microbial Relationships. Wicklow DT and Soderstorm B, eds. pp 165-184.
Chet I (1987). Trichoderma-application, mode of action and potential as a biocontrol agent of soil borne plant pathogenic fungi: Chet I., (Ed). Innovative Approaches to Plant Disease Control. John Wiley as sans. New York. pp137-160.
Dubos B, Bulit J (1981). Filamentous fungi as biocontrol agents on aerial plant surfaces Ill:. BlackmanJ.P. (ed). Microbial Ecology of the phyl/oplalle. Academic Press, London, pp 353-356.
Deshmukh AJ, Mehta BP, Patil VA (2010). In vitro evaluation of some known bioagents to control C. gloeosporioides Penz, and Sacc, causing Anthracnose of Indian bean. Int. J. Pharma and Bio sci. 1 (2) pp 1-6
Elad Y, Zimand G (1992). Integration of biological and chemical control of grey mould. In: N. E. Malathrakis, and B. Williarnson (eds) Recent Advances ill Botrytis Research. Pudoc Scientific Publishers, Wageningen, Netherlands. . pp 272-276.
Elad Y., Zimand G., Zoqs Y., Zuriel S., and Chet I., (1993). Use of Trichoderma harzianum in combination or elternation with fungicides to control cucumber gray mold (Botrytis cinerea) under commercial green house conditions. Plant Pathology. 42:324-332
Faheem A, Razdan VK, Mohiddin FA, Bhat KA, Sheikh PA (2010). Effect of volatile metabolites of Trichoderma Spp. against seven fungal plant pathogens in vitro. J. Phytol. 2(10): 34-37.
Fahim MM, Attia MF, Okasha AK, Abada KA ( 1989). Trichoderma as a biocontrol agent against root and crown-rots of strawberry. Egypt. J. Phytopathol., 21 (2): 139-148 .
Hanounik SB, Hassanein AM (1986). Inhibition of Botrytis fabae in the phyllosphere of Vicia fabae leave. Fabis Newsletter 16:41-44.
Hansen HN (1926). A simple method of obtaining single spore cultures. Science (N.S.), 64: 384.
Hmouni A, Hajlaoui MR, Mlaiki A (1996). Resistance de Botrytis cinerea aux benzimidazoles et aux dicarboximides dans les cultures de tomate en Tunisie. OEPP /EPPO Bulletin, 26: 697¬705.
Jarvis WR (1977). Botryotinia and Botrytis species: Taxonomy, Physiology and Pathogenicity, pp. 33-34. Monograph 15, Research Branch, Canada Department of Agricultural Science, Ottawa.
Kumar RN, Mukerji KG (1996). Integrated disease management future perspectives, pp. 335-347. In: K.G. Mukerji, B. Mathur, B.P. Chamala and C. Chitralekha (Eds.), Advances in Botany.APH Publishing Corporation, New Delhi.
Nagendra B, Prasad, Kumar MR (2011). Effect of non-volatile compounds produced by Trichoderma spp. on growth and sclerotial viability of Rhizoctonia solani, incitant of sheath blight of rice. Indian J. Fundamental Appl. Life Sci.1 (2) 37-42.
Nassib AM, Khalil SA, El-Botai MA, Radi MM (1991). In: Nile Valley Regional program on Cool Season Food Legumes and Cereals.1990/91.Annual Report, Egypt. Cairo: ARC /ICARDA.
Mahmoud NA, Ashrei AAM, Mekhemar GAA (2012). Evaluation of some products in controlling chocolate spot of faba bean and their effect on the growth and yield parameters. Egypt .J. Agric. Res., 90 (3): 991-1000.
Patel JJ, Brown ME (1969). Interactions of Azobacter with rhisosphere and root-surface microflora. Plant and Soil, 31: 273¬281
Saber WIA, Abd El-Hai KM, Ghoneem KM (2009). Synergistic effect of Trichoderma and Rhizobium on Both biocontrol of chocolate spot disease and induction of nodulation physiological activities and productivity of Vicia faba. Res. J. Microbiol 4:286-300.
Sahile S, Sakhuja PK, Fininsa C, Ahmed S (2011). Potential antagonistic fungal species from Ethiopia for biological control of chocolate spot disease of faba bean. Syria African Crop Science J. Afr. Crop Sci. Society, 19 (3): 213 - 225.
Schirmbock M, Lorito M, Wang YL, Hayes CK, Arisan-Atlac I, Scala F, Harman GE, Kubicek CP (1994). Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi. Applied Environmental Microbioliogy, 60: 4364-4370.
Svetlana Z, Stojanovic S, Ivanovic Z, Gavrilovic V, Tatjana P, Jelica Balaz (2010). Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides, Archives of Biological Science., Belgrade, 62(3) :611-623.
Silva FA. Azevedo CA (2009). Principal Components Analysis in the Software Assistat-Statistical Attendance. In: Worled Congress on Computers in Agriculture, 7 Reno-NV-USA: American Society of Agricultural and Biological Engineers.
Vey A, Hoagland RE, Butt TM, (2001). Toxic metabolites of fungal biocontrol agents. Fungi as Biocontrol Agents: Progress, Problems and Potential. Butt T.M, Jackson C and N, eds, pp 311¬346. CAB International, Bristol.
Zimand G, Elad Y, Chet I (1996). Effect of Trichoderma harzianum on Botrytis cinerea pathogenicity. Phytopathology, 86: 11, 1255¬1260.