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Effect of Combination among Bioagents, Compost and Soil Solarization on Management of Strawberry Fusarium Wilt

Received: 17 December 2014    Accepted: 25 December 2014    Published: 1 February 2015
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Abstract

Isolation trials from strawberry plants showing mainly wilt symptoms grown at Behera, Ismailia, Kalubia and Giza governorates yielded Fusarium oxysporum , F.solani , Macrophomina phaseolina , Pythium ultimum , Phytophthora cactorum , Rhizoctonia solani and Sclerotium rolfsii . Pathogenicity test of the four isolates of F.oxysporum revealed that they caused wilt symptoms and Kalubia isolate was the most virulent one. In addition, inoculating different seven plants, i.e. bean, cucumber, eggplant, sweet pepper, strawberry, tomato and water melon with F. oxysporum isolate of Kalubia governorate indicated that it caused wilt symptoms to strawberry plants only. Therefore, it named Fusarium oxysporum f.sp. fragariae Winks & Y.N. Williams. Four isolates of Bacillus spp., i.e. Bacillus coagulans , B.humilus , B.subtils and B. thringiensis and one isolate of Pseudomonas flurescens were isolated from the rhizospheric soil of strawberry plants grown in a field have severe infection by Fusarium wilt were screened for their efficacy against F. o. f.sp. fragariae, in vitro and in vivo. In general, P.flurescens followed by Bacillus subtilis were the most efficient in reducing the linear growth of the pathogenic fungus. Sterilized aqueous filtrate of the tested compost resulted in significant reduction to the linear growth of the tested fungus compared with control treatment. This reduction was gradually increased by increasing it׳s concentration. The combination among the bioagents B.subtilis and P.flurescens, compost and soil solarization resulted in significant reduction to strawberry Fusarium wilt with significant increase to the produced fruits and their total soluble solids (T.S.S.) , either each of them was used alone or in their different combinations, compared with control treatment (infested with the causal fungus).On the other hand, compost was the most efficient in this regard compared with the other three items of disease management, i.e. soil solarization and the bioagents B.subtilis and P.flurescens when each of them was used alone. Moreover, no apparent infection was detected when the bioagents B.subtilis and P.flurescens ,compost and soil solarization were used together and produced fruit yield of T.S.S., to somewhat, similar to control treatment ( uninfested soil with the causal fungus).

Published in American Journal of Life Sciences (Volume 2, Issue 6-2)

This article belongs to the Special Issue Role of Combination Between Bioagents and Solarization on Management of Crown-and Stem-Rot of Egyptian Clover

DOI 10.11648/j.ajls.s.2014020602.16
Page(s) 39-46
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bacterial Bioagents, Compost, Fruit Yield, Management, Strawberry and Total Soluble Solids

References
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    Abada K. A., Faten M. Abd-El-Latif, Hala A.M. El-Dakar. (2015). Effect of Combination among Bioagents, Compost and Soil Solarization on Management of Strawberry Fusarium Wilt. American Journal of Life Sciences, 2(6-2), 39-46. https://doi.org/10.11648/j.ajls.s.2014020602.16

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    Abada K. A.; Faten M. Abd-El-Latif; Hala A.M. El-Dakar. Effect of Combination among Bioagents, Compost and Soil Solarization on Management of Strawberry Fusarium Wilt. Am. J. Life Sci. 2015, 2(6-2), 39-46. doi: 10.11648/j.ajls.s.2014020602.16

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    Abada K. A., Faten M. Abd-El-Latif, Hala A.M. El-Dakar. Effect of Combination among Bioagents, Compost and Soil Solarization on Management of Strawberry Fusarium Wilt. Am J Life Sci. 2015;2(6-2):39-46. doi: 10.11648/j.ajls.s.2014020602.16

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  • @article{10.11648/j.ajls.s.2014020602.16,
      author = {Abada K. A. and Faten M. Abd-El-Latif and Hala A.M. El-Dakar},
      title = {Effect of Combination among Bioagents, Compost and Soil Solarization on Management of Strawberry Fusarium Wilt},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {6-2},
      pages = {39-46},
      doi = {10.11648/j.ajls.s.2014020602.16},
      url = {https://doi.org/10.11648/j.ajls.s.2014020602.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020602.16},
      abstract = {Isolation trials from strawberry plants showing mainly wilt symptoms grown at Behera, Ismailia, Kalubia and Giza governorates yielded Fusarium oxysporum , F.solani , Macrophomina phaseolina , Pythium ultimum , Phytophthora cactorum , Rhizoctonia solani and Sclerotium rolfsii . Pathogenicity test of the four isolates of F.oxysporum revealed that they caused wilt symptoms and Kalubia isolate was the most virulent one. In addition, inoculating different seven plants, i.e. bean, cucumber, eggplant, sweet pepper, strawberry, tomato and water melon with F. oxysporum isolate of Kalubia governorate indicated that it caused wilt symptoms to strawberry plants only. Therefore, it named Fusarium oxysporum f.sp. fragariae Winks & Y.N. Williams. Four isolates of Bacillus spp., i.e. Bacillus coagulans , B.humilus , B.subtils and B. thringiensis and one isolate of Pseudomonas flurescens were isolated from the rhizospheric soil of strawberry plants grown in a field have severe infection by Fusarium wilt were screened for their efficacy against F. o. f.sp. fragariae, in vitro and in vivo. In general, P.flurescens followed by Bacillus subtilis were the most efficient in reducing the linear growth of the pathogenic fungus. Sterilized aqueous filtrate of the tested compost resulted in significant reduction to the linear growth of the tested fungus compared with control treatment. This reduction was gradually increased by increasing it׳s concentration. The combination among the bioagents B.subtilis and P.flurescens, compost and soil solarization resulted in significant reduction to strawberry Fusarium wilt with significant increase to the produced fruits and their total soluble solids (T.S.S.) , either each of them was used alone or in their different combinations, compared with control treatment (infested with the causal fungus).On the other hand, compost was the most efficient in this regard compared with the other three items of disease management, i.e. soil solarization and the bioagents B.subtilis and P.flurescens when each of them was used alone. Moreover, no apparent infection was detected when the bioagents B.subtilis and P.flurescens ,compost and soil solarization were used together and produced fruit yield of T.S.S., to somewhat, similar to control treatment ( uninfested soil with the causal fungus).},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Effect of Combination among Bioagents, Compost and Soil Solarization on Management of Strawberry Fusarium Wilt
    AU  - Abada K. A.
    AU  - Faten M. Abd-El-Latif
    AU  - Hala A.M. El-Dakar
    Y1  - 2015/02/01
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajls.s.2014020602.16
    DO  - 10.11648/j.ajls.s.2014020602.16
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 39
    EP  - 46
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2014020602.16
    AB  - Isolation trials from strawberry plants showing mainly wilt symptoms grown at Behera, Ismailia, Kalubia and Giza governorates yielded Fusarium oxysporum , F.solani , Macrophomina phaseolina , Pythium ultimum , Phytophthora cactorum , Rhizoctonia solani and Sclerotium rolfsii . Pathogenicity test of the four isolates of F.oxysporum revealed that they caused wilt symptoms and Kalubia isolate was the most virulent one. In addition, inoculating different seven plants, i.e. bean, cucumber, eggplant, sweet pepper, strawberry, tomato and water melon with F. oxysporum isolate of Kalubia governorate indicated that it caused wilt symptoms to strawberry plants only. Therefore, it named Fusarium oxysporum f.sp. fragariae Winks & Y.N. Williams. Four isolates of Bacillus spp., i.e. Bacillus coagulans , B.humilus , B.subtils and B. thringiensis and one isolate of Pseudomonas flurescens were isolated from the rhizospheric soil of strawberry plants grown in a field have severe infection by Fusarium wilt were screened for their efficacy against F. o. f.sp. fragariae, in vitro and in vivo. In general, P.flurescens followed by Bacillus subtilis were the most efficient in reducing the linear growth of the pathogenic fungus. Sterilized aqueous filtrate of the tested compost resulted in significant reduction to the linear growth of the tested fungus compared with control treatment. This reduction was gradually increased by increasing it׳s concentration. The combination among the bioagents B.subtilis and P.flurescens, compost and soil solarization resulted in significant reduction to strawberry Fusarium wilt with significant increase to the produced fruits and their total soluble solids (T.S.S.) , either each of them was used alone or in their different combinations, compared with control treatment (infested with the causal fungus).On the other hand, compost was the most efficient in this regard compared with the other three items of disease management, i.e. soil solarization and the bioagents B.subtilis and P.flurescens when each of them was used alone. Moreover, no apparent infection was detected when the bioagents B.subtilis and P.flurescens ,compost and soil solarization were used together and produced fruit yield of T.S.S., to somewhat, similar to control treatment ( uninfested soil with the causal fungus).
    VL  - 2
    IS  - 6-2
    ER  - 

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Author Information
  • Plant Pathol. Dept., Fac. Agric., Cairo Univ., Giza, Egypt

  • Agric. Botany Dept., Fac. Agric., Benha Univ.,Moshtohor, Egypt

  • Integ. Cont. Res. Dept., Plant Pathol.Res. Instit., Agric. Res. Centre, Giza, Egypt

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