American Journal of Life Sciences

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Identification and Application of Drosophila Novel Bioactive Peptides dRYamides

Received: 25 March 2015    Accepted: 02 April 2015    Published: 06 May 2015
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Abstract

The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals.

DOI 10.11648/j.ajls.s.2015030302.12
Published in American Journal of Life Sciences (Volume 3, Issue 3-2, May 2015)

This article belongs to the Special Issue Biology and Medicine of Peptide and Steroid Hormones

Page(s) 3-7
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Novel Bioactive Peptide, Orphan GPCR, Drosophila

References
[1] Vassilatis DK, Hohmann JB, Zeng H, Li F, Ranchalis JE, Mortrud MT, Brown A, Rodriguez SS, Weller JR, Wright AC, Bergmann JE, Gaitanaris G. The G protein-coupled receptor repertories of human and mouse, Proc Natl Acad Sci USA 2003; 100: 4903-4908.
[2] Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF. The genome sequence of Drosophila melanogaster, Science 2000; 287: 2185-2195.
[3] Brody T, Cravchik A. Drosophila melanogaster G protein-coupled receptors, J Cell Biol. 2000; 150: 83-88.
[4] Konopka RJ, Benzer S. Clock mutants of Drosophila melanogaster, Proc Natl Acad Sci U S A, 1971; 68(9): 2112-2116.
[5] Anderson KV, Jürgens G, Nüsslein-Volhard C. Establishment of dorsal-ventral polarity in the Drosophila embryo: genetic studies on the role of the Toll gene product,Cell 1985; 42(3): 779-789.
[6] Lemaitre BI, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA. The dorsoventral regulatory gene cassette spätz le/Toll/cactus controls the potent antifungal response in Drosophila adults, Cell 1996; 86(6): 973-983.
[7] Poltorak AI, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B, Beutler B. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene,Science 1998; 282(5396): 2085-2088.
[8] Beck B. KO's and organisation of peptidergic feeding behavior mechanisms, Neurosci. Biobehav. Rev. 2001; 25: 143–158.
[9] Williams G, Bing C, Cai XJ, Harrold JA, King PJ, Liu XH. The hypothalamus and the control of energy homeostasis: Different circuits, different purposes, Physiol. Behav. 2001; 74: 683–701.
[10] Zimanyi IA, Fathi Z, Poindexter GS. Central control of feeding behavior by neuropeptide Y, Curr. Pharm. Des. 1998; 4: 349–366.
[11] Nässel DR, Wegener C. A comparative review of short and long neuropeptide F signaling in invertebrates: Any similarities to vertebrate neuropeptide Y signaling? Peptides. 2011; 326(6): 1335-55.
[12] Garczynski SF, Brown MR, Shen P, Murray TF, Crim JW. Characterization of a functional neuropeptide F receptor from Drosophila melanogaster, Peptides. 2002; 23: 773–780.
[13] Feng G, Reale V, Chatwin H, Kennedy K, Venard R, Ericsson C, Yu K, Evans PD, Hall LM. Functional characterization of a neuropeptide F-like receptor from Drosophila melanogaster, Eur. J. Neurosci. 2003; 18: 227–238.
[14] Mertens I, Meeusen T, Huybrechts R, Loof AD, Schoofs L. Characterization of the short neuropeptide F receptor from Drosophila melanogaster, Biochem. Biophys. Res. Commun. 2002; 297: 1140–1148.
[15] Li XJ, Wu YN, North RA, Forte M. Cloning, functional expression, and developmental regulation of a neuropeptide Y receptor from Drosophila melanogaster, J. Biol. Chem. 1992; 267: 9–12.
[16] Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Ozaki M, Hiraguchi T, Maeda T, Shiotani H, Terajima S, Sano H, Mori K, Yoshida M, Miyazato M, Kato J, Murakami N, Kangawa K.Kojima M. Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila, Biochem Biophys Res Commun. 2011; 410: 872-77.
[17] Civelli O, Nothacker HP, Saito Y, Wang Z, Lin SH, Reinscheid RK. Novel neurotransmitters as natural ligands of orphan G-protein-coupled receptors, Trends Neurosci. 2001; 24: 230-237.
[18] Brody T, Cravchik A. Drosophila melanogaster G protein-coupled receptors, J Cell Biol. 2000; 150: 83-88.
[19] Ida T, Miyazato M, Lin XZ, Kaiya H, Sato T, Nakahara K,Murakami N, Kangawa K, Kojima M. Purification and characterization of caprine ghrelin and its effect on growth hormone release, J. Mol. Neurosci. 2010; 42: 99–105.
[20] Nisimura T, Seto A, Nakamura K, Miyama M, Nagao T, Tamotsu S, Yamaoka R, Ozaki M. Experiential effects of appetitive and nonappetitive odors on feeding behavior in the blowfly, Phormia regina: A putative role for tyramine in appetite regulation, J. Neurosci. 2005; 25: 7507–7516.
Author Information
  • Department of Bioactive Peptides, Frontier Science Research Center University of Miyazaki, Miyazaki, Japan

  • Department of Bioactive Peptides, Frontier Science Research Center University of Miyazaki, Miyazaki, Japan

  • Institute of Life Sciences, Kurume University, Fukuoka, Japan

  • Institute of Life Sciences, Kurume University, Fukuoka, Japan

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  • APA Style

    Takanori Ida, Eri Iwamoto, Takahiro Sato, Masayasu Kojima. (2015). Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. American Journal of Life Sciences, 3(3-2), 3-7. https://doi.org/10.11648/j.ajls.s.2015030302.12

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    ACS Style

    Takanori Ida; Eri Iwamoto; Takahiro Sato; Masayasu Kojima. Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. Am. J. Life Sci. 2015, 3(3-2), 3-7. doi: 10.11648/j.ajls.s.2015030302.12

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    AMA Style

    Takanori Ida, Eri Iwamoto, Takahiro Sato, Masayasu Kojima. Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. Am J Life Sci. 2015;3(3-2):3-7. doi: 10.11648/j.ajls.s.2015030302.12

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  • @article{10.11648/j.ajls.s.2015030302.12,
      author = {Takanori Ida and Eri Iwamoto and Takahiro Sato and Masayasu Kojima},
      title = {Identification and Application of Drosophila Novel Bioactive Peptides dRYamides},
      journal = {American Journal of Life Sciences},
      volume = {3},
      number = {3-2},
      pages = {3-7},
      doi = {10.11648/j.ajls.s.2015030302.12},
      url = {https://doi.org/10.11648/j.ajls.s.2015030302.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.s.2015030302.12},
      abstract = {The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Identification and Application of Drosophila Novel Bioactive Peptides dRYamides
    AU  - Takanori Ida
    AU  - Eri Iwamoto
    AU  - Takahiro Sato
    AU  - Masayasu Kojima
    Y1  - 2015/05/06
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajls.s.2015030302.12
    DO  - 10.11648/j.ajls.s.2015030302.12
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 3
    EP  - 7
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2015030302.12
    AB  - The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals.
    VL  - 3
    IS  - 3-2
    ER  - 

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