Steroid Hormones as Transporters to Carry Exogenous Macromolecules into the Target Cell Nuclei in Vivo
American Journal of Life Sciences
Volume 3, Issue 3-2, May 2015, Pages: 53-57
Received: Jan. 29, 2015; Accepted: Apr. 1, 2015; Published: May 6, 2015
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Toshikazu Nishimura, Aichi Medical University School of Medicine, Yazako, Nagakute, Aichi 480-1195, Japan
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Upon injection into the vascular system of rats, testosterone-bovine serum albumin conjugate (testosterone-BSA) is taken up by cells via the process of endocytosis. When it is taken up by the target cells of testosterone such as spermatogenic cells, it enters the nuclei of the cells. However, testosterone-BSA does not enter the nuclei of the non-target cells such as hepatocytes and thymocytes. Similarly, hydrocortisone-BSA conjugate enters the nuclei of its target cells such as hepatocytes and thymocytes. In the vesicular trafficking of testosterone-BSAs into the nucleoplasm, the vesicle membrane is likely to fuse with a nuclear hemifusion diaphragm. IgG coupled with hydrocortisone also enters the hormone-target cell nuclei, with its antigenicity kept intact. These results suggest that steroid hormones could act as transporters for conveying exogenous macromolecules into the target cell nuclei in vivo. Our studies provide a novel insight to the functions of steroid hormones.
Steroid-Protein Conjugates, Vesicular Trafficking, Nuclear Diaphragm, Target Cell Nuclei, Intact Antigenicity, In Vivo
To cite this article
Toshikazu Nishimura, Steroid Hormones as Transporters to Carry Exogenous Macromolecules into the Target Cell Nuclei in Vivo, American Journal of Life Sciences. Special Issue:Biology and Medicine of Peptide and Steroid Hormones. Vol. 3, No. 3-2, 2015, pp. 53-57. doi: 10.11648/j.ajls.s.2015030302.20
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