Phytochemical Extraction and Screening of Bio Active Compounds from Black Cumin (Nigella Sativa) Seeds Extract
American Journal of Life Sciences
Volume 3, Issue 5, October 2015, Pages: 358-364
Received: Jul. 29, 2015;
Accepted: Aug. 12, 2015;
Published: Aug. 24, 2015
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Abdurohaman Mengesha Yessuf, Department of Chemistry, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia
This study is interested to now a day's attracting attention of the researchers; which is natural products and their derivatives because of they are becoming sources to important drugs and the pharmaceutical industries have come to consider traditional medicine as a source of bioactive agents that can be used in the preparation of synthetic medicines and helpful also as nutritional values which are very interesting in their eco-friendness and free of the toxicity. Base on this, the current investigation is directed to the detection of the bioactive Black cumin which is one of the miraculous plant having multifarious roles in its phytochemical constituents and nutritional values, treating digestive tract conditions including gas, colic, diarrhea, dysentery, constipation, and hemorrhoids. Sopowdered Nigella sativa seed was used for crude oil extracts by using different solvents. In this manner, the results of investigation of qualitative phytochemical analysis conducted on the crude cumin seeds extract revealed the presence of bioactive compounds in the petroleum ether, ethyl acetate and methanol extracts which are known to exhibit medicinal as well as physiological activities. Identification and separations were taken by TLC and CC. Finally four potentially active phytochemicals have been obtained from methanol extracts; are alkaloids, phenol, flavonoids and steroids. Thus we can scientifically conclude that the cumin seeds extract could be seen as an increasingly valuable reservoir of bioactive compounds of substantial medicinal merit
Abdurohaman Mengesha Yessuf,
Phytochemical Extraction and Screening of Bio Active Compounds from Black Cumin (Nigella Sativa) Seeds Extract, American Journal of Life Sciences.
Vol. 3, No. 5,
2015, pp. 358-364.
Majidul Haque, Mst. Marium Begum, Moynul Hasan, A. F. M. Towheedur Rahman, Md. Iftekhar Hussain, Mohammad Mizanur Rahman, Md. Hazrat Ali, Md. Ashraful Islam, Md. Zakir Sultan, Md. Reyad ul Ferdous, Choudhury Mahmood Hasan. Investigation of the Medicinal Potentials of Syzygium jambos (L.) Extract and Characterization of the Isolated Compounds. American Journal of Bio Science. Special Issue: Pharmacological and Phytochemicals Investigation. Vol.3, No. 2-1, 2015, pp. 12-18. doi: 10.11648/j.ajbio.s.2015030201.13.
Fahad M. A. and S. J. Mohammed, 2012. Chemical Composition and Fatty Acid Content of Some Spices and Herbs under Saudi Arabia Conditions. The Scientific World Journal Vol. (1) p. 1.
Farooq T. J. and M. A. Abdulrahman, 2011. The effects of Nigella sativa oil administration on some physiological and histological values of reproductive aspects of rats. Iraqi J. Vet. Med. 35 (2) p. 53.
Aftab A. A., M.M. Husain, A.S. Nasir, A.D. Zoheir and B.H. Anil Bhandari, 2014. Physicochemical and phytochemical standardization with HPTLC fingerprinting of Nigella sativa L. seeds. Pak. J. Pharm. Sci. Vol.27, p. 1175.
Kamal M.E., S.E. Nareman, S.H. Randa, 2013. Physicochemical Characteristics, Nutrient Content and Fatty Acid Composition of Nigella sativa Oil and Sesame Oil. Food and Public Health.3 (6).p.309.
HalaG.M., E.N. Nahed, S.S. Regine, 2006. The Medicinal Potential of Black Seed (Nigella Sativa) and Its Components. Khan And A. Ather (Eds.) Lead Molecules From Natural Products. VO (1) p. 134.
Ali G.H. andZ. E. Jaafar, 2014. Optimization of Reflux Conditions for Total Flavonoid and Total Phenolic Extraction and Enhanced Antioxidant Capacity in Pandan Using Response Surface Methodology. Scientific World Journal vol(1).
Aftab A.,H. Asif, M. S. Mohd S. A. Khan, K.N. Abul,A.S. Nasir and A. Firoz, 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Journal List.Asian Pac. J. Trop. Biomed vol. 3(5).
Hamburger, M. and K. Hostettmaun, 1991. Bioactivity in plants. The link between phytochemistry and medicine. Phytochem., 30 (12): p. 3864-3874.
Azra Kamal, 2014. Phytochemical Screening of Syzygium Cumini Seeds. Indian Journal Of Plant Sciences: 2319–3824. Vol. 3 (4). p.1.
Madhukar. C, 2013. Phytochemical screening of cumin seeds extract. Rep. Opinion, Karnataka, India. V. 5(1):57-58. (ISSN: 1553-9873).
Abdurohman Mengesha Yessuf, Dr. Masood Akhtar Khan, Phytochemical Investigation and Characterization on the Fruit Peel of Solanummarginatum (methanol extract), AARJMD Vol. 1, No 33, 2015, pp. 184-195
Sibi G., Apsara Venkategowda, Lepakshi Gowda. Isolation and Characterization of Antimicrobial Alkaloids from Plumeriaalba Flowers against Food Borne Pathogens. American Journal of Life Sciences. Special Issue: Recent Developments in Health Care through Plants and Microbes Vol. 2, No. 6-1, 2014, pp. 1-6. doi: 10.11648/j.ajls.s.2014020601.11.