American Journal of Life Sciences
Volume 3, Issue 6-1, November 2015, Pages: 69-75
Received: Sep. 30, 2015;
Accepted: Oct. 7, 2015;
Published: Nov. 29, 2015
Views 4734 Downloads 89
Mohamed A. Amer, Faculty of Science, Al-Azhar University, Cairo, Egypt
Awaad A. M. El-Sayed, Faculty of Science, Al-Azhar University, Cairo, Egypt
Khalid A. Al-Damhougy, Faculty of Science, Al-Azhar University, Cairo, Egypt
Samir A. Zaahkouk, Faculty of Science, Al-Azhar University, Cairo, Egypt
Eyestalk ablation was used for inducing molting in the freshwater crayfish, Procambarus clarkii at the laboratory conditions. The results exhibited that, both of unilateral and bilateral eyestalk removal accelerated molting rate than in non-ablated or intact individuals of this species. The use of unilateral eyestalk removal has low effect, induced molting at the 6th week and reached 100% at the end of the 16th week, compared with starting molting at the 8th week for control, which did not exceed than 40 % for molted individuals at the end of experiment, with 20% mortality for each. On contrast, the bilateral ablation has high effects and induced molting rapidly at the first and second weeks for the two experiments. It was associated with high mortality rates, averaged 28.33%for ablated individuals of the two experiments, increased gradually to 51.67%and 80.0% for individuals of the premolt stages, which showed signs of molting but were unable to complete molting cycle and died at the end of the last week of each experiment. Repeating molting was recorded for only one bilateral ablated animal after 11 days, but no molting was noticed for control group, with mortality of 20 %.The percentages of increments in body dimensions included body length, carapace length, chelae length and total body weight were calculated. Most ratios of increments, except only total body weight, were high in ablated males than ablated females, as well as clearly higher in normal molted males and females than ablated ones, but declined sharply to 3.30±2.91% in total body weight for bilateral ablated males.
Mohamed A. Amer,
Awaad A. M. El-Sayed,
Khalid A. Al-Damhougy,
Samir A. Zaahkouk,
Inducing Molting by Eyestalk Ablation in the Red Swamp Crayfish, Procambarus clarkii from the Egyptian Freshwaters, American Journal of Life Sciences. Special Issue: New Horizons in Basic and Applied Zoological Research.
Vol. 3, No. 6-1,
2015, pp. 69-75.
Huner, J.V. and Lindqvist, O. V.: Special problems in freshwater crayfish egg production. In: Crustacean egg production, (eds. A. Wenner and A. Kuris), 1995, pp. 235-264.
Huner, J.V. Moody, M. and Thure, R.: Cultivation of freshwater crayfish aquaculture in North America, Europe and Eustralia. Families Astacidae, Cambridae and Parastacidae. Haworth press, New York, 1993.
Ibrahim, A.M.; Khalil, M.T. and Mubarak, M.F.: On the feeding behavior of the Exotic crayfish, P. clarkia in Egypt and its prospects in the bicontrol of local vector snails. . J, Union Arab Biol., Cairo. Vol.4,No. A, 1995, pp 321-340.
Ibrahim, A.M. and Khalil, M.T.: The red swamp crayfish in Egypt. (A fast spreading freshwater invasive crustacean), Egypt. Centre of Research & Studies of Protectorates, Ain Shams Unv.Vol.1,2009, pp: 153.
Ibrahim, A. M.; Emam W. M.; Beltagy S. M.; Tharwat, A. A. and Mubarak F. M.: On some factor affecting molting of the crayfish Procambarus clarkii from the River Nile Egypt. J. Environ. Sci., vol.1, No.1, 2000, pp23-45.
Mona, M.H.; Geasa, N.M. Sh.; Sharshar, Kh. M. and Morsy, E.M.: Chemical composition of freshwater crayfish (Procambarus clarkii) and its nutritive value. Egypt. J. Aquat. Biol. And Fish.,vol.4, No.1,1999, pp 19 – 34.
Raafat, H. A.: Biological and physiological studies on the freshwater crayfish, Procambarus clarkii, Ph. D. Thesis, Department of Zoology, Girls College for Arts, Science and Education, Ain Shams University, 2006, 267 pp.
Warner, G. F.: The Biology of crabs. Elek Science, London, 1977, pp119-140.
Chang, E. S.: Physiological and biochemical changes during the molt cycle in decapod crustaceans. J. Exp. Mar. Biol. & Ecol., vol. 193, 1995, 1-14.
Brown, F. A. and Cunningham, O.: Influence of sinus gland of crustaceans in normal ability and ecdysis. Biol. Bull. Woods Hole, vol. 77, 1939, pp 104-114.
Carlisle, D. B.: Moulting hormones in Leander (Crustacea: decapoda). J. mar. Biol. Assoc., U. K. vol. 32, 1953 a, pp289-296.
Adiyodi, K. J. and Adiyodi, R. J.: Endocrine control of reproduction in decapod Crustacea, Biol. Rev., vol. 45, 1970, pp 121-165.
Nakatani, I. and Otsu, T.: The relationship between growth and molt interval in the eyestalkless crayfish, P. clarkia. Comp. Biochem. Physiol., vol. 68, No. A, 1979, pp549-553.
Jegla, T. C.; Ruland C.; Kegel G.; and Keller R. (): The role of the Y-organ and cephalic gland in ecdysteroid production and the control of molting in the crayfish, Orconectes limosus, USA, Comp Physiol., vol. 152, 1983, pp91-95.
Naya, Y. and Ikeda, M.: Comprehensive survey of endogenous regulators of crustacean moulting. Pure & Appl. Cham.,Vol. 65, No.6, 1993, pp1265-1270.
Chen S.; Huner, J. V. and Malone, R. F.: Moulting and mortality of red swamp and White River crawfish subjected to eyestalk ablation: Preliminary study for commercial soft-shell crawfish production. Journal of the world Aquaculture Socity, vol. 24, 1993, pp48- 57.
Chen S., Wu, J. and Malone R. F. (): Effects of temperature on mean molt interval, molting and mortality of red swamp crawfish’ (Procambarus clarkii), Aquaculture, vol. 131,1995, pp. 205-217.
Venkitraman, P.R.; Jayalakshmy, K.V.; Balasubramanian, T.; Nair, M. and Nair, K.K.C.: Effect of eyestalk ablation on the growth and moulting of penaeid prawns Metapenaeus dobsoni(de Man), Indian J. Exp. Biol., vol.42, 2004, pp. 403-412.
Venkitraman, P. R.; Jayalakshmy, K. V.; Balasubramanian, T.: Effect of eyestalk ablation on molting and growth in penaeid prawns Metapenaeus monoceros. Indian J. Fish., vol. 57, No. 2, 2010, pp. 25-32.
Hesni, M. A.; Shabanipour, N.; Atabati, A. and Bitaraf, A. (): Influence of eyestalk ablation and temperature on molting and mortality of Narrow-clawed crayfish (Astacus leptodactylus).Turkish Journal of Fisheries and Aquatic Sciences, vol. 8, 2008, pp. 219-223.
Bittnerand, G. D. and Kopanda R.(): Factors influencing molting in the crayfish Procambarus clarkii. J. of Experimental Zoology; Vol. 186, issue"1,1995, pp. 7 – 16.
Ibrahim, A. M.; Emam W. M.; Mubarak F. M.; Beltagy S. M. and Tharwat, A. A.: On the reproductive biology of the crayfish Procambarus clarkii from the River Nile Egypt. J. Egypt, Acad., Environ. develop., vol. 7, No. 1, 2006, pp. 145-171.
Sainz-Hernándeza, J. C.; Racotta, I. S.; Dumas, S. and Hernández - López, J.: Effect of unilateral and bilateral eyestalk ablation in Litopenaeus vannamei male and female on several metabolic and immunologic variables, Elsevier, Aquaculture, vol. 283(1-4),2008, pp. 188-193.
Lowery, R.S.: Growth, molting and reproduction. In: Freshwater Crayfish, Biology, Management and Exploitation (Holdich, D.M. and Lowery, R.S., Eds.). Croom Helm Press, London, 1988, pp. 83–113.
Ranga Rao, K.: Isolation and partial characterization of the moult –inhibiting hormone of the crustacean eyestalk. Experientia, vol. 21, 1965, pp. 593-594.
Passano, L. M.: Low temperature blocking of moulting in Uca pugnax, Biol. Bull., vol. 188, 1960, pp. 129-136.
Yamaoka, L. H. and Scheer, B. T.: Chapter: 11: Chemistry of growth and development in crustaceans. In: Chemical Zoology; Academic Press, London, Vol. (A) 1970, pp. 321-338.
Willing, A. and Keller, R.: Moulting hormone content, cuticle growth and gastrolith growth in the molt cycle of the crayfish Orconectes limosus. J. comp. Physiol., vol. 86,1973, pp. 377-388.
Okumura, T. and Katsumi, A.: Effect of bilateral eyestalk ablation on moulting and ovarian development in the giant freshwater prawn, Macrobarchium rosenbergii, J. Fresh. Sci., vol. 67, 2001, pp. 11- 25.
Aiken, D. E., Waddy, S. S., Castell, J. D. and Covey, J. F.: Effect of sex, temperature, diet and holding conditions on growth of intact and eyestalk ablated lobsters (Homarus americanus). Division Fish Oceanography, 1977, pp. 7: 13.
Aiken, D. E., Waddy, S. S.: Molting and growth in crayfish: a review .Can. Tech. Rep. Fish. Aquat. Sci., 587, 1987, 34pp.Effect of sex, temperature, diet and holding conditions on growth of intact and eyestalk ablated lobsters (Homarus americanus). Division Fish Oceanography, 7: 13.
Gydemon, R. and Westin, L.: Investigation on eyestalk ablation in Astacus astacus. J. Aquat. Product., vol. 2, 1988, pp. 155-171.
Huner, J.V. and Lindqvist, O. V.: Effect of temperature and diet on reproductively active male noble crawfish (Astacus astacus) subjected to bilateral eyestalk ablation. J. of the world aquaculture Soc., vol. 15, 1994, pp. 138-141.
Zaib Un Nisa Ahmed, M.: Natural and inducing spawning of Pakistan Penaeid shrimps in the laboratory, Aquaculture, vol. 33, No. 2, 2001, pp. 125-130.