For sustainable space exploration there is need for torrential food supply. Apart from food in storage, constant production is vital as this could also serve as a source of Oxygen when activated during space expedition. However, the impact of gravity in crop development is significant. This study therefore, investigated the morphological and physiological responses of Maize (Zea mays) seedlings subjected to clinorotation. Agar Agar was used as source of nutrient for the developing seedlings. The Agar was prepared by measuring 1 – 1.5 g and made up to 100 ml with tap water. Seeds were collected in the seed bank of the Space-Earth Environment Research Laboratory, Benin City. Three sets of petri dishes were prepared and marked; one for normal surface, one rotated at 90°C and another set for clinorotation. Those for clinorotation were rotated at three different times; with 1, 2, and 3 rpm for 6 hrs respectively. The plumule, radicle and Enzyme activities were measured and analysed after four days. Results showed significant difference in germination parameters as occasioned by microgravity. Where as clinorotation enhanced radicule length (1.8-2.1cm), effect on plumule was minimal (p>0.05). Significant increase in CAT activity in the plumule was reported (7.59mol/sec) in the clinorotated (1rpm) seed compared to the control (2.56mol/sec). MDA activity in both radicle and plumule were higher than that of the control (p<0.05). Microscopic study of the cells was carried out using a binocular microscope (Labo) with a camera and result showed that the normal surface sample cells were arranged concentrically with spaces, while that of clinorotated seeds were arranged concentrically but highly packed with little spaces for the plumule. For the radicle, the normal surface sample cells were scattered within the plant with more spaces, while that of clinorotated were mostly clustered throughout.
| Published in | American Journal of Life Sciences (Volume 9, Issue 1) |
| DOI | 10.11648/j.ajls.20210901.13 |
| Page(s) | 11-18 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
EA: Enzyme Activities, MDA: Malondealdehyde, Clinorotation
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APA Style
Alexander Oseghale Orukpe, Geofrey Obinna Anoliefo, Beckley Ikhajiagbe. (2021). Effects of Clinorotation on the Enzyme Activities and Morphology of Zea mays Seedlings. American Journal of Life Sciences, 9(1), 11-18. https://doi.org/10.11648/j.ajls.20210901.13
ACS Style
Alexander Oseghale Orukpe; Geofrey Obinna Anoliefo; Beckley Ikhajiagbe. Effects of Clinorotation on the Enzyme Activities and Morphology of Zea mays Seedlings. Am. J. Life Sci. 2021, 9(1), 11-18. doi: 10.11648/j.ajls.20210901.13
AMA Style
Alexander Oseghale Orukpe, Geofrey Obinna Anoliefo, Beckley Ikhajiagbe. Effects of Clinorotation on the Enzyme Activities and Morphology of Zea mays Seedlings. Am J Life Sci. 2021;9(1):11-18. doi: 10.11648/j.ajls.20210901.13
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Download@article{10.11648/j.ajls.20210901.13,
author = {Alexander Oseghale Orukpe and Geofrey Obinna Anoliefo and Beckley Ikhajiagbe},
title = {Effects of Clinorotation on the Enzyme Activities and Morphology of Zea mays Seedlings},
journal = {American Journal of Life Sciences},
volume = {9},
number = {1},
pages = {11-18},
doi = {10.11648/j.ajls.20210901.13},
url = {https://doi.org/10.11648/j.ajls.20210901.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20210901.13},
abstract = {For sustainable space exploration there is need for torrential food supply. Apart from food in storage, constant production is vital as this could also serve as a source of Oxygen when activated during space expedition. However, the impact of gravity in crop development is significant. This study therefore, investigated the morphological and physiological responses of Maize (Zea mays) seedlings subjected to clinorotation. Agar Agar was used as source of nutrient for the developing seedlings. The Agar was prepared by measuring 1 – 1.5 g and made up to 100 ml with tap water. Seeds were collected in the seed bank of the Space-Earth Environment Research Laboratory, Benin City. Three sets of petri dishes were prepared and marked; one for normal surface, one rotated at 90°C and another set for clinorotation. Those for clinorotation were rotated at three different times; with 1, 2, and 3 rpm for 6 hrs respectively. The plumule, radicle and Enzyme activities were measured and analysed after four days. Results showed significant difference in germination parameters as occasioned by microgravity. Where as clinorotation enhanced radicule length (1.8-2.1cm), effect on plumule was minimal (p>0.05). Significant increase in CAT activity in the plumule was reported (7.59mol/sec) in the clinorotated (1rpm) seed compared to the control (2.56mol/sec). MDA activity in both radicle and plumule were higher than that of the control (p<0.05). Microscopic study of the cells was carried out using a binocular microscope (Labo) with a camera and result showed that the normal surface sample cells were arranged concentrically with spaces, while that of clinorotated seeds were arranged concentrically but highly packed with little spaces for the plumule. For the radicle, the normal surface sample cells were scattered within the plant with more spaces, while that of clinorotated were mostly clustered throughout.},
year = {2021}
}
TY - JOUR T1 - Effects of Clinorotation on the Enzyme Activities and Morphology of Zea mays Seedlings AU - Alexander Oseghale Orukpe AU - Geofrey Obinna Anoliefo AU - Beckley Ikhajiagbe Y1 - 2021/03/22 PY - 2021 N1 - https://doi.org/10.11648/j.ajls.20210901.13 DO - 10.11648/j.ajls.20210901.13 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 11 EP - 18 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20210901.13 AB - For sustainable space exploration there is need for torrential food supply. Apart from food in storage, constant production is vital as this could also serve as a source of Oxygen when activated during space expedition. However, the impact of gravity in crop development is significant. This study therefore, investigated the morphological and physiological responses of Maize (Zea mays) seedlings subjected to clinorotation. Agar Agar was used as source of nutrient for the developing seedlings. The Agar was prepared by measuring 1 – 1.5 g and made up to 100 ml with tap water. Seeds were collected in the seed bank of the Space-Earth Environment Research Laboratory, Benin City. Three sets of petri dishes were prepared and marked; one for normal surface, one rotated at 90°C and another set for clinorotation. Those for clinorotation were rotated at three different times; with 1, 2, and 3 rpm for 6 hrs respectively. The plumule, radicle and Enzyme activities were measured and analysed after four days. Results showed significant difference in germination parameters as occasioned by microgravity. Where as clinorotation enhanced radicule length (1.8-2.1cm), effect on plumule was minimal (p>0.05). Significant increase in CAT activity in the plumule was reported (7.59mol/sec) in the clinorotated (1rpm) seed compared to the control (2.56mol/sec). MDA activity in both radicle and plumule were higher than that of the control (p<0.05). Microscopic study of the cells was carried out using a binocular microscope (Labo) with a camera and result showed that the normal surface sample cells were arranged concentrically with spaces, while that of clinorotated seeds were arranged concentrically but highly packed with little spaces for the plumule. For the radicle, the normal surface sample cells were scattered within the plant with more spaces, while that of clinorotated were mostly clustered throughout. VL - 9 IS - 1 ER -
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