The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice
American Journal of Life Sciences
Volume 1, Issue 2, April 2013, Pages: 55-60
Received: May 12, 2013;
Published: May 30, 2013
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Torricelli P., Department SPES, University of Molise, Campobasso, Italy
Ferorelli P., Department SPES, University of Molise, Campobasso, Italy
De Martino A., University of Rome, Tor Vergata, Dept Biology, Roma, Italy
Antonelli F., University of Rome, Tor Vergata, Dept Biology, Roma, Italy
Beninati S., University of Rome, Tor Vergata, Dept Biology, Roma, Italy
Liver steatosis development was obtained after high cholesterol diet (HCD) in C57BL6/N mice. Mice were preliminarily treated for 10 days with multiple micronutrients contained in a commercial food supplement called Citozym (CIZ), and successively for 40 days with CIZ and HCD, to compare the protection effect of used compounds on liver metabolism and metabolic steatosis. At the end of the experiments, livers were dissected for histological examinations. Plasma total cholesterol (TCH), triacylglycerol (TAG) aspartate aminotransferase (AST), and alanine aminotransferase (ALT) concentrations were significantly higher in all HCD-fed mice. These data support a lipotoxic model of cholesterol-mediated hepatic steatosis. We found that the administration of CIZ to HCD-mouse model of steatosis significantly decreased plasma TCH, TAG, ALT and AST levels along with a reduction of the accumulated fat and inflammation in the liver. These findings suggest that a preventive and continuative treatment of CIZ exerts a protective effect against metabolic hepatosteatosis.
De Martino A.,
The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice, American Journal of Life Sciences.
Vol. 1, No. 2,
2013, pp. 55-60.
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