The Efficacy of Trichoderma spp. and Bacillus Isolates in the Control of Chickpea Wilt Pathogens
Agriculture, Forestry and Fisheries
Volume 3, Issue 5, October 2014, Pages: 346-351
Received: Aug. 30, 2014; Accepted: Sep. 15, 2014; Published: Sep. 30, 2014
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Authors
Hanan Ibrahim Mudawi, The Environmental, Natural Resources and Desertification Research Institute, National Centre for Research, Ministry of Science and Technology, Khartoum, Sudan
Mohamed Osman Idris, Department of Plant Protection, College of Agriculture, Khartoum University, Khartoum, Sudan
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Abstract
Dual experiments were carried out in 2007 at the laboratories of the National Center of Research, to test the antagonistic efficacy of three Trichoderma spp and 23 Bacillus isolates, for the control of chickpea wilt and root- rot pathogens: Fusarium oxysporum f. sp. ciceris and F. solani adopting CRD. Trichoderma harzianum was found highly antagonistic compared to Trichoderma viride isolates as it inhibited the mycelial growth of F. oxysporum f. sp. ciceris and F. solani by 85.29% and 86.21% after 12 days of in-vitro incubation, whereas T. viride (isolate Tv1) gave an inhibition percentage of 81.88% and 76.64%. Antagonistic hyphae of T. harzianum showed parasitic behavior against Fusarium spp. The parasite reached and recognized F. oxysporum f. sp. ciceris by coiling around the hyphae of the pathogen and disintegrating the hyphae and spores. Only 17 out of 23 Bacillus isolates from 130 colonies of bacteria screened showed significantly antagonistic properties against wilt pathogens. Only B3, B16, B2, B15and B20 proved to be the most effective among the rest of isolates and were considered strongly antagonistic against F. oxysporum f. sp. ciceris and F. solani in-vitro, with an inhibition percentage range of 57.57% - 64.65%. The management of Chickpea root/rot wilt complex disease incited by F. oxysporum f. sp. ciceris and F. solani could be achieved successively by the use of bioagents derived from various fungal and bacterial isolates.
Keywords
Antagonisms, Bacillus spp, Biocontrol, Cicer arietinum L, Trichoderma harzianum, T. viride
To cite this article
Hanan Ibrahim Mudawi, Mohamed Osman Idris, The Efficacy of Trichoderma spp. and Bacillus Isolates in the Control of Chickpea Wilt Pathogens, Agriculture, Forestry and Fisheries. Vol. 3, No. 5, 2014, pp. 346-351. doi: 10.11648/j.aff.20140305.13
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