Sugar-Induced Tolerance to the Salt Stress in Maize Seedlings by Balancing Redox Homeostasis
Agriculture, Forestry and Fisheries
Volume 5, Issue 4, August 2016, Pages: 126-134
Received: Aug. 5, 2016; Published: Aug. 8, 2016
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Authors
Zhao Ying, Department of Agronomy, College of Agronomy Heilongjiang Bayi Agricultural University, Daqing, China
Xu Jing-yu, Department of Agronomy, College of Agronomy Heilongjiang Bayi Agricultural University, Daqing, China; Department of Agronomy, Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilongjiang Province Education Department, Daqing, China
He Lin, Department of Agronomy, College of Agronomy Heilongjiang Bayi Agricultural University, Daqing, China; Department of Agronomy, Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilongjiang Province Education Department, Daqing, China
Yang Ke-jun, Department of Agronomy, College of Agronomy Heilongjiang Bayi Agricultural University, Daqing, China; Department of Agronomy, Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilongjiang Province Education Department, Daqing, China
Zhao Chang-jiang, Department of Agronomy, College of Agronomy Heilongjiang Bayi Agricultural University, Daqing, China; Department of Agronomy, Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilongjiang Province Education Department, Daqing, China
Wang Zhi-hui, Department of Agronomy, College of Agronomy Heilongjiang Bayi Agricultural University, Daqing, China; Department of Agronomy, Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilongjiang Province Education Department, Daqing, China
Li Zuo-tong, Department of Agronomy, College of Agronomy Heilongjiang Bayi Agricultural University, Daqing, China; Department of Agronomy, Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilongjiang Province Education Department, Daqing, China
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Abstract
Effects of exogenous glucose (Glc) and sucrose (Suc) on salt resistance, (glucose-6-phosphate dehydrogenase) G6PDH activity, ASA-GSH cycle and reactive oxygen species metabolism in maize leaves were studied. Seedings at two leaf stage were pretreated by 1/2 Hoagland nutrient solution with 0.5 mmol•L-1 Glc, Suc and mannitol for 3 days, some seedlings were left in normal nutrient solution and isotonic maninitol as control and permeability control respectively. Then the plants were cultured by 1/2 Hoagland solution at the concentration of 150 mmol•L-1 NaCl for 4 days or 6 days. The results showed that pretreatment with 0.5 mmol•L-1 glucose and sucrose for 3 days significantly decreased the thiobarbituric acid reactive substances (TBARS), H2O2 contents and increased ASA, GSH contents, ASA/DHA, GSH/GSSG ratio and G6PDH activity in leaves of maize seedings under salt stress. We also found that sucrose pretreatment induced the increase of Ca2+-ATPase and maintained the balance of Ca2+ contents under salt stress. In addition, compared with S treatment, isotonic mannitol pretreatment did not induce any changes of the physiological and biochemical indicators that meant it could not cause improvement of biological indicators of maize seedings. These results indicate that exogenous glucose and sucrose improved ASA-GSH cycle efficiency by participating in the pentose phosphate pathway (OPP), therefore enhanced antioxidant capacity and reduced the damage of active oxygen free radical, and the G6PDH activity induced by glucose and sucrose might serve as signal molecules and be involved in salt resistance of maize seedings.
Keywords
Maize (Zea mays L.), Glucose, Sucrose, Salt Stress, Glucose-6-Phosphate Dehydrogenase, Pentose Phosphate Pathway
To cite this article
Zhao Ying, Xu Jing-yu, He Lin, Yang Ke-jun, Zhao Chang-jiang, Wang Zhi-hui, Li Zuo-tong, Sugar-Induced Tolerance to the Salt Stress in Maize Seedlings by Balancing Redox Homeostasis, Agriculture, Forestry and Fisheries. Vol. 5, No. 4, 2016, pp. 126-134. doi: 10.11648/j.aff.20160504.15
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