Effect of Micro-Remediation on Enzymes Activity and Available Nutrients in Chlorpyrifos-Polluted Soils
Agriculture, Forestry and Fisheries
Volume 6, Issue 5, October 2017, Pages: 166-172
Received: Sep. 21, 2017;
Published: Sep. 22, 2017
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Junhua Wu, College of Life Sciences, Sichuan Normal University, Chengdu, China
Xin Chen, College of Life Sciences, Sichuan Normal University, Chengdu, China
Chuanzhou Ou, College of Life Sciences, Sichuan Normal University, Chengdu, China
Chunping Huang, College of Life Sciences, Sichuan Normal University, Chengdu, China; Sichuan Engineering Center for Farmland Ecosystem Service Capacity Construction, Institute of Agro-products Processing, Chengdu, China
In order to find out the effect of micro-remediation on enzyme activity and available nutrient in chlorpyrifos-polluted soils and the correlation of enzyme activity, available nutrients and chlorpyri fos residue during soil remediation process, the soils planting Brassica juncea throughout the year, sprayed with chlorpyrifos and treated with bacteria were selected as restoration land, while the soils only sprayed with chlorpyrifos were regarded as reference land. The results showed that the activities of urease, catalase, amylase and phosphatase in the restoration land were higher than those in the reference land, especially in the subsequent phase of sample time (P < 0.05). Correlation analysis demonstrated that there was significantly negative correlation between amylase activity and chlorpyrifos residue in the soils only sprayed chlorpyrifos, while amylase and catalase activity had a significantly negative correlation with chlorpyrifos residue respectively in the restoration soils (P < 0.01). Meanwhile, just as the soil enzyme activities, the available nutrients content in the restoration soils were higher than those in the soils only sprayed chlorpyrifos, and significant difference for available phosphorus between the two soils (P < 0.05) can be observed. Correlation analysis demonstrated that there was no significant correlation between available nutrients and chlorpyrifos residue in the reference soils, while available phosphorus content was significantly negatively correlated with chlorpyrifos residue in the restoration soils (P < 0.01). In general, the microbial remediation can contribute to effectively enhancing the contents of available nitrogen, available potassium and available phosphorus in soils polluted by chlorpyrifos and improve soil fertility well.
Effect of Micro-Remediation on Enzymes Activity and Available Nutrients in Chlorpyrifos-Polluted Soils, Agriculture, Forestry and Fisheries.
Vol. 6, No. 5,
2017, pp. 166-172.
Xu G., Zheng W., Li Y., et al. Biodegradation of chlorpyrifos and 3,5,6-tricholoro-2-pyridinol by a newly isolated Paracoccus sp. strain TRP. Int Biodeter Biodegr, 2008, (62): 51-56.
Randhanwa M. A., Anjum F. M., Ahmed A., et al. Field incurred chlorpyrifos and 3,5,6-trichloro-2-pyridinol residues in fresh and processed vegetables. Food Chem, 2007, (103): 1016-1023.
Cai E. X. Residual dynamics and safety assessment of chlorpyrifos in banana and orchard soil. Journal of Safety and Environment, 2015, 15(4): 349-352.
Zhang J. Y., Liu L. L., Li G. C., et al. Oxidative stress effects of chlorpyrifos on zebrafish embryos. China Environmental Science, 2016, 36(3): 927-934.
Duan H. M. Factors influencing the biodegradation of chlorpyrifos by a Bacillus cereus strain. Journal of Safety and Environment, 2015, 15(2): 174-177.
Yang Z. H., Wu R. P., Wang B., et al. Bioremediation of Cr (Ⅵ) contaminated soil and pilot study. Environmental Chemistry, 2013, 32(9): 1758-1765.
Yao X. H., Min H., Lu Z. H., et al. Influence of acetamiprid on soil enzymatic activities and respiration. European Journal of Soil Biology, 2006, 42(2): 120-126.
Yang W. B., Geng Y. Q., Wang D. M. The activities of soil enzyme under different vegetation types in Li river riparian ecotones. Acta Ecologica Sinica, 2015, 35(14): 1-12.
Huang C. Y. Soil sciences. Beijing: China Agricultural Press, 2000.
Luo F. Z. Xiang L., Li H., et al. Effects of arbuscular mycorrhizal fungi (AMF) on growth and Cd accumulation of upland rice and soil enzyme activities in cadmium contaminated soil. Journal of Agro-Environment Science, 2015, 34(6): 1090-1095.
Cai Z. Q., Li S. S., Zhang W. S. Effects of the novel pyrimidynyl oxybenzoic herb-icide ZJ0273 on enzyme activities, microorganisms and its degradation in Chinese soils. Environmental Science and Pollution Research, 2015, 22(6): 4425-4433.
Thomas P., Markus D. M., Hans R. B., et al. Environmental behavior of the chiral herbicide haloxyfop. 1 rapid and preferential interconversion of the enantiomers in soil. J. Agric. Food Chem., 2015, 63(10): 2583-2590.
Huang C. P., LI J., LI Y. L., et al. Effect of applying Bacillus subtilis MZS1 strain on degradation of chlorpyrifos residue in Brassica juncea and soil. Guizhou Agricultural Sciences, 2016, 44(4): 66-70.
Luo M., Chen X., Shu P., et al. Simultaneous determination of indoxacarb and chlorpyrifos residues in Brassica oleracea by high performance liquid chromatography. Journal of Mountain Agriculture and Biology, 2014, 33(1): 5-7.
Zheng H. Y., Zhang D. S. Dynamic biochemical study of soil. Beijing: China Science Press, 1982.
Bao S. D. Soil Agricultural and Chemicals Analysis Method. Beijing: China Agriculture Press, 2000.
Zhao Z. Q., Hou X. W., Li Q. F., et al. Effects of chlorpyrifos and carbosulfan on soil enzymes activity in banana rhizosphere. Journal of Agro-Environment Science, 2010, 29, 100-101.
Wang H. J., Zhu N. W., Yang C., et al. Effect of soil enzyme activities during bioremediation of crude oil-contaminated soil. Journal of Agro-Environment Science, 2013, 32(6): 1178-1184.
Dai W., Bai H. Y. Correlations of soil catalase activity and it’s kinetic characteristic with some soil properties. Journal of Beijing Forestry University, 1995, 17(9): 37-41.
Zheng X. B., Fan J. B., Zhou J., et al. Effects of combined application of biogas slurry and chemical fertilizer on soil nutrients and peanut yield in upland red soil. Acta Pedologica Sinica, 2016, 53(3): 675-684.
Zheng M. H., Huang J., Chen H., et al. Effects of nitrogen and phosphorus addition on soil phosphatase activity in different forest types. Acta Ecologica Sinica, 2015, 35(20): 6703-6710 (in Chinese).
Nannipieri P., Giagnoni L., Landi L., et al. Role of phosphatase enzymes in soil // Phosphorus in Action. Berlin Heidelberg: Springer, 2011.
Wu X. H., Xu J., Dong F. S., et al. Effects of five herbicides on activities of soil invertase and urease. Chinese Journal of Pesticide Science, 2015, 17(2): 179-184.
Shan M., Fang H., Wang X., et al. Effect of chlorpyrifos on soil microbial populations and enzyme activities. Journal of Environmental Sciences, 2006, 18(1): 4-5.
Wang L. M., Xu D. M., Chen B., et al. Effects of external contaminants on soil phosphatase. Techniques and Equipment for Environmental Pollution Control, 2004, 5(5): 11-17.
Zeng Q. B., Li T., Wang C. Q., et al. Microbial Agents: Effects on Activities of Urease and Catalase in Flue-cured Tobacco Rhizosphere Soil. Chinese Agricultural Science Bulletin, 2016, 32(22): 46-50.
Li H., Chen Y. X., Liang X. Q., et al. Influence of soil urease activities on nitrogen conversion in floodwater in paddy field. Journal of Soil and Water Conservation, 2015, 20(1): 55-58.
Chen Q. C., Xu F, L., Wang W. L., et al. Seasonal dynamics of available K in soil for different ages of Larix principis-rupprechtii in the northern foot of the Qinling. Journal of Plant Nutrition and Fertilizer, 2014, 20(5): 1243-1249.
John E. M., Shaike J. M. Chlorpyrifos: Pollution and remediation. Environmental Chemistry Letters, 2015, 13(3): 269-291.
Zhang L. H., Li K., Hu X. X., et al. Effect of microbial agents on the available nutrient and enzyme activity of grape replant soil. Northern Horticulture, 2015, (4): 162-164.