Effects of Salinity on Some Haematological and Biochemical Parameters in Nile Tilapia, Oreochromus niloticus
Agriculture, Forestry and Fisheries
Volume 6, Issue 6, December 2017, Pages: 200-205
Received: Sep. 15, 2017;
Accepted: Sep. 26, 2017;
Published: Nov. 5, 2017
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Naglaa Elarabany, Zoology Department, Faculty of Science, Damietta University, Damietta, Egypt
Mohammed Bahnasawy, Zoology Department, Faculty of Science, Damietta University, Damietta, Egypt
Gamal Edrees, Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt
Rajab Alkazagli, Zoology Department, Faculty of Science, Damietta University, Damietta, Egypt; Zoology Department, Faculty of Science, Al-Asmariya University for Islamic Sciences, Zliten, Libya
Any change in haematological and biochemical parameters could be a predictor of unfavorable environment or effect of different stress factors. The present study was designed to assess different salinity concentration induced changes in some haematological and biochemical parameters in 64 O. niloticus fishes captured from Manzala Lake (Egypt), they were acclimatized and fed with commercial fish diet for one week before starting the experiment for another 2 weeks. Fishes were divided into 4 equal groups assigned as; control, 4g NaCl\L, 8 g NaCl\L and 12 g NaCl\L. RBCs, HCT, Hb concentration, platelets count, superoxide dismutase activity, catalase activity, potassium level and serum total protein were estimated. The HCT, Hb concentration, platelets count and potassium levels were significantly higher in (4gNaCl\L, 8gNaCl\L and12gNaCl\L, respectively). The Superoxide dismutase activity (SOD), total protein, RBCs and catalase activit were significantly lower in (4gNaCl\L, 8g NaCl\L and 12gNaCl\L, respectively) compared to the control group. Other parameters such as WBCs, haematimetric indices (MCV, MCH, MCHC), malonedialdhyde (MDA) level, carbonyl protein (CP), glutathione reductase (GR) activity, as well as serum sodium, glucose, cortisol and IgM, did not show any significant differences in the estimated salinity concentrations.
Effects of Salinity on Some Haematological and Biochemical Parameters in Nile Tilapia, Oreochromus niloticus, Agriculture, Forestry and Fisheries.
Vol. 6, No. 6,
2017, pp. 200-205.
Macfadyen G, Nasr-Alla AM, Al‐Kenawy D, Fathi M, Hebicha H, Diab AM, et al. Value-chain analysis—An assessment methodology to estimate Egyptian aquaculture sector performance. Aquaculture 2012; 362:18-27.
de Azevedo RV, de Oliveira KF, Flores-Lopes F, Teixeira-Lanna EA, Takishita SS, Tavares-Braga LG. Responses of Nile tilapia to different levels of water salinity/Respuestas de la tilapia del Nilo a diferentes niveles de salinidad del agua. Latin American Journal of Aquatic Research 2015; 43:828.
Schulte PM. What is environmental stress? Insights from fish living in a variable environment. Journal of Experimental Biology 2014; 217:23-34.
Hamid SA, Ahmed FM, Mohammed IA, Ali SM. Physical & Chemical Characteristics of Blood of two Fish Species (Oreochromis niloticus and Clarias lazera). World’s Veterinary Journal 2013; 3:17-20.
Carragher JF, Rees CM. Primary and secondary stress responses in golden perch, Macquaria ambigua. Comparative Biochemistry and Physiology Part A: Physiology 1994; 107:49-56.
McDonald G, Milligan L. Ionic, osmotic and acid-base regulation in stress. Fish stress and health in aquaculture 1997; 62:119-145.
Ruscoe IM, Shelley CC, Williams GR. The combined effects of temperature and salinity on growth and survival of juvenile mud crabs (Scylla serrata Forskål). Aquaculture 2004; 238:239-247.
Larsen PF, Nielsen EE, Meier K, Olsvik PA, Hansen MM, Loeschcke V. Differences in salinity tolerance and gene expression between two populations of Atlantic cod (Gadus morhua) in response to salinity stress. Biochemical genetics 2012;50:454-466.
Hasenbein M, Komoroske LM, Connon RE, Geist J, Fangue NA. Turbidity and salinity affect feeding performance and physiological stress in the endangered delta smelt. Integrative and comparative biology 2013;53:620-634.
Kültz D. Physiological mechanisms used by fish to cope with salinity stress. Journal of Experimental Biology 2015; 218:1907-1914.
Sinha AK, AbdElgawad H, Zinta G, Dasan AF, Rasoloniriana R, Asard H, et al. Nutritional status as the key modulator of antioxidant responses induced by high environmental ammonia and salinity stress in European sea bass (Dicentrarchus labrax). PloS one 2015; 10:e0135091.
El‐Saidy DM, Gaber M. Effect of dietary protein levels and feeding rates on growth performance, production traits and body composition of Nile tilapia, Oreochromis niloticus (L.) cultured in concrete tanks. Aquaculture research 2005; 36:163-171.
El-Zaeem SY, Ahmed MMM, Salama M, El-Maremie H. Production of salinity tolerant Nile tilapia, Oreochromis niloticus through traditional and modern breeding methods: II. Application of genetically modified breeding by introducing foreign DNA into fish gonads. African Journal of Biotechnology 2011; 10:684-695.
Brett J, Groves T. 6 Physiological Energetics. Fish physiology 1979; 8:279-352.
Wootton RJ. Introduction. Ecology of teleost fishes: Springer; 1990. p. 1-14.
Barton BA, Iwama GK. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annual Review of Fish Diseases 1991; 1:3-26.
Yada T, Uchida K, Kajimura S, Azuma T, Hirano T, Grau E. Immunomodulatory effects of prolactin and growth hormone in the tilapia, Oreochromis mossambicus. Journal of endocrinology 2002; 173:483-492.
Ninh NH, Thoa NP, Knibb W, Nguyen NH. Selection for enhanced growth performance of Nile tilapia (Oreochromis niloticus) in brackish water (15–20ppt) in Vietnam. Aquaculture 2014; 428:1-6.
de Oca GAR-M, Román-Reyes JC, Alaniz-Gonzalez A, Omar C, Serna-Delval GM-C, Rodríguez-González H. Effect of salinity on three tilapia (Oreochromis sp.) strains: hatching rate, length and yolk sac size. Int J Aqu Sci 2015; 6:96-106.
Moorman BP, Lerner DT, Grau EG, Seale AP. The effects of acute salinity challenges on osmoregulation in Mozambique tilapia reared in a tidally changing salinity. Journal of Experimental Biology 2015; 218:731-739.
Tran-Ngoc KT, Schrama JW, Le MT, Nguyen TH, Roem AJ, Verreth JA. Salinity and diet composition affect digestibility and intestinal morphology in Nile tilapia (Oreochromis niloticus). Aquaculture 2016.
Boeuf G, Payan P. How should salinity influence fish growth? Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2001; 130:411-423.
Enayat Gholampoor T, Imanpoor M, Shabanpoor B, Hosseini S. The Study of Growth Performance, Body Composition and Some Blood Parameters of Rutilus frisii kutum (Kamenskii, 1901) Fingerlings at Different Salinities. Journal of Agricultural Science and Technology 2011; 13:869-876.
Imsland AK, Gústavsson A, Gunnarsson S, Foss A, Árnason J, Arnarson I, et al. Effects of reduced salinities on growth, feed conversion efficiency and blood physiology of juvenile Atlantic halibut (Hippoglossus hippoglossus L.). Aquaculture 2008; 274:254-259.
Usha R. EFFECT OF SALINITY CHANGES ON HAEMATOLOGICAL PARAMETERS OF THE TIGER SHARK PANGASIUS HYPOPHTHALMUS. Journal of Ecobiology 2011; 29:283.
Fazio F, Marafioti S, Arfuso F, Piccione G, Faggio C. Influence of different salinity on haematological and biochemical parameters of the widely cultured mullet, Mugil cephalus. Marine and Freshwater Behaviour and Physiology 2013; 46:211-218.
Soltanian S, Vazirzadeh A, Fallahi R. Effects of sudden salinity changes on short-term hematological and biochemical responses in Mudskipper Periophthalmus waltoni Koumans 1941 (Gobiidae: Perciformes). Iranian Journal of Ichthyology 2016; 3:31-42.
Alwan S, Hadi A, Shokr A. Alterations in hematological parameters of fresh water fish, Tilapia zillii, exposed to aluminum. J Sci Its Appl 2009; 3:12-19.
Gabriel U, Anyanwu P, Anyanwu A, Akinrotimi A. Effect of freshwater challenge on the blood characteristics of Sarotherodon melanotheron. Agricultural Journal 2007; 2:388-391.
Akinrotimi O, Agokei E, Aranyo A. Changes in blood parameters of Tilapia guineensis exposed to different salinity levels. Journal of Environmental Engineering and Technology 2012; 1:4-12.
Svobodová Z, Flajšhans M, Kolářová J, Modrá H, Svoboda M, Vajcová V. Leukocyte profiles of diploid and triploid tench, Tinca tinca L. Aquaculture 2001;198:159-168.
Sahafi HH, Masaeli S, Alizadeh M, Negarestan H, Naji T. A study on growth parameters, blood factors and proximate composition of rainbow trout (Oncorhynchus mykiss) cultured in underground brackish and freshwater. Iranian Journal of Fisheries Sciences 2013;12:836-842, IV.
Amin FB. EFFECTS OF SALINITY ON PHYSIOLOGICAL AND BEHAVIORAL STRESS RESPONSES IN SILVER BARB (Barbonymus gonionotus). PhD Thesis Bangladesh Agricultural University, Mymensingh.; 2014.
Semple JW, Italiano JE, Freedman J. Platelets and the immune continuum. Nature Reviews Immunology 2011;11:264-274.
Stosik M, Deptula W, Travnicek M. Studies on the number and ingesting ability of thrombocytes in sick carps (Cyprinus carpio L.). VETERINARNI MEDICINA-PRAHA- 2001;46:12-16.
Karadag H, Fırat Ö, Fırat Ö. Use of oxidative stress biomarkers in Cyprinus carpio L. for the evaluation of water pollution in Ataturk Dam Lake (Adiyaman, Turkey). Bulletin of environmental contamination and toxicology 2014;92:289-293.
Wang F, Yang H, Gao F, Liu G. Effects of acute temperature or salinity stress on the immune response in sea cucumber, Apostichopus japonicus. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2008;151:491-498.
Boya P, de la Peña A, Beloqui O, Larrea E, Conchillo M, Castelruiz Y, et al. Antioxidant status and glutathione metabolism in peripheral blood mononuclear cells from patients with chronic hepatitis C. Journal of hepatology 1999;31:808-814.
Mazeaud MM, Mazeaud F, Donaldson EM. Primary and secondary effects of stress in fish: some new data with a general review. Transactions of the American Fisheries Society 1977;106:201-212.
Karsi A, Yildiz HY. Secondary stress response of nile tilapia, Oreochromis niloticus, after direct transfer to different salinities. Tarim Bilimleri Dergisi 2005;11:139-141.
Benli A, Yildiz HY. Alteration of renal tissue in Nile tilapia, Oreochromis niloticus, after transfer to saline water. 2004.
Semra K, Karul A, Yildirim Ş, Gamsiz K. Effects of salinity on growth and metabolism in blue tilapia (Oreochromis aureus). African Journal of Biotechnology 2013;12.
Vijayan M, Mommsen T, Glémet H, Moon T. Metabolic effects of cortisol treatment in a marine teleost, the sea raven. Journal of Experimental Biology 1996;199:1509-1514.
Kavya K, Jadesh M, Kulkarni R. Hematology and serum biochemical changes in response to change in saline concentration in fresh water fish Notopterus notopterus. World Scientific News 2016;32:49.
Sangiao-Alvarellos S, Laiz-Carrión R, Guzmán JM, del Río MPM, Miguez JM, Mancera JM, et al. Acclimation of S. aurata to various salinities alters energy metabolism of osmoregulatory and nonosmoregulatory organs. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2003;285:R897-R907.
Sangiao-Alvarellos S, Arjona FJ, del Río MPM, Míguez JM, Mancera JM, Soengas JL. Time course of osmoregulatory and metabolic changes during osmotic acclimation in Sparus auratus. Journal of Experimental Biology 2005;208:4291-4304.
Yılmaz S, Ergün S. Effects of garlic and ginger oils on hematological and biochemical variables of sea bass Dicentrarchus labrax. Journal of aquatic animal health 2012;24:219-224.
Kelly S, Woo N. The response of sea bream following abrupt hyposmotic exposure. Journal of fish biology 1999;55:732-750.
Martinez-Alvarez R, Hidalgo M, Domezain A, Morales A, García-Gallego M, Sanz A. Physiological changes of sturgeon Acipenser naccarii caused by increasing environmental salinity. Journal of experimental biology 2002;205:3699-3706.
Harris J, Bird DJ. Modulation of the fish immune system by hormones. Veterinary immunology and immunopathology 2000;77:163-176.
Cataldi E, Di Marco P, Mandich A, Cataudella S. Serum parameters of Adriatic sturgeon Acipenser naccarii (Pisces: Acipenseriformes): effects of temperature and stress. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 1998;121:351-354.
Fiúza LS, Aragão NM, Junior R, Pinto H, Moraes MG, Rocha ÍRCB, et al. Effects of salinity on the growth, survival, haematological parameters and osmoregulation of tambaqui Colossoma macropomum juveniles. Aquaculture Research 2015;46:1-9.
Khairnar SO, Tian X, Dong S, Fang Z, Solanki BV, Shanthanagouda HA. Effects of the amplitude and frequency of salinity fluctuations on antioxidant responses in juvenile tongue sole, Cynoglossus semilaevis. Spanish Journal of Agricultural Research 2016;14:0503.
Louis-Cormier ES, Osterland C, Anderson PD. Evidence for a cutaneous secretory immune system in rainbow trout (Salmogairdneri). Developmental & Comparative Immunology 1984;8:71-80.